Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March-2023 Volume 25 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2023 Volume 25 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Heterogeneity of adherent and non‑adherent JAWS II dendritic cells

  • Authors:
    • Marta Maruszewska‑Cheruiyot
    • Maja Machcińska
    • Magdalena Kierasińska
    • Katarzyna Donskow‑Lysoniewska
  • View Affiliations / Copyright

    Affiliations: Department of Experimental Immunotherapy, Faculty of Medicine, Lazarski University, 02‑662 Warsaw, Poland, Laboratory of Parasitology, General Karol Kaczkowski Military Institute of Hygiene and Epidemiology, 01‑163 Warsaw, Poland
  • Article Number: 93
    |
    Published online on: January 25, 2023
       https://doi.org/10.3892/ol.2023.13680
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Dendritic cells (DCs) are crucial in the development of immune responses. DC JAWS II is a murine cell line frequently used in DC studies. These cells are grown in two cell fractions: Adherent and non‑adherent. The present study aimed to compare these two fractions in both immature and lipopolysaccharide (LPS)‑activated JAWS II cells. The present study analysed the condition, phenotype, antigen uptake capability, signalling properties and the influence on the activity of T cells using flow cytometry, mixed cell reaction and ELISA methods. Adherent immature JAWS II cells exhibited increased endocytosis and decreased activation of the Pi3K signalling pathway. After LPS activation, adherent JAWS II cells exhibited increased expression levels of CD80 and CD86 costimulatory molecules, increased endocytosis and an elevated ability to induce T cell proliferation, compared with non‑adherent cells. These results demonstrated that the two fractions of JAWS II adherent and non‑adherent cells exhibited different properties and this should be taken into account in the planning of research.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Vang KB, Safina I, Darrigues E, Nedosekin D, Nima ZA, Majeed W, Watanabe F, Kannarpady G, Kore RA, Casciano D, et al: Modifying dendritic cell activation with plasmonic nano vectors. Sci Rep. 7:55132017. View Article : Google Scholar : PubMed/NCBI

2 

Zapala L, Drela N, Bil J, Nowis D, Basak GW and Lasek W: Optimization of activation requirements of immature mouse dendritic JAWSII cells for in vivo application. Oncol Rep. 25:831–840. 2011.PubMed/NCBI

3 

Jittimanee S, Wongratanacheewin S, Kaewraemruaen C and Jittimanee J: Opisthorchis viverrini antigens up-regulates the expression of CD80 and MHC class II in JAWSII mouse dendritic cells and promotes IL-10 and TGF-β secretions. Parasitol Int. 84:1024012021. View Article : Google Scholar : PubMed/NCBI

4 

Maruszewska-Cheruiyot M, Donskow-Łysoniewska K, Piechna K, Krawczak K and Doligalska M: L4 stage Heligmosomoides polygyrus prevents the maturation of dendritic JAWS II cells. Exp Parasitol. 196:12–21. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Bieńkowska A, Kiernozek E, Kozlowska E, Zarzycki M and Drela N: Thymus-deriving natural regulatory T cell generation in vitro: Role of the source of activation signals. Immunol Lett. 162:199–209. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Eko FO, Mania-Pramanik J, Pais R, Pan Q, Okenu DM, Johnson A, Ibegbu C, He C, He Q, Russell R, et al: Vibrio cholerae ghosts (VCG) exert immunomodulatory effect on dendritic cells for enhanced antigen presentation and induction of protective immunity. BMC Immunol. 15:5842014. View Article : Google Scholar : PubMed/NCBI

7 

Egger M, Jürets A, Wallner M, Briza P, Ruzek S, Hainzl S, Pichler U, Kitzmüller C, Bohle B, Huber CG and Ferreira F: Assessing protein immunogenicity with a dendritic cell line-derived endolysosomal degradome. PLoS One. 6:e172782011. View Article : Google Scholar : PubMed/NCBI

8 

Hu Y, Li M, Lu B, Wang X, Chen C and Zhang M: Corticotropin-releasing factor augments LPS-induced immune/inflammatory responses in JAWSII cells. Immunol Res. 64:540–547. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Mutlu EC, Kaya Ö, Wood M, Mager I, Topkara KÇ, Çamsarı Ç, Birinci Yildirim A, Çetinkaya A, Acarel D and Odabaşı Bağcı J: Efficient doxorubicin loading to isolated dexosomes of immature JAWSII cells: Formulated and characterized as the bionanomaterial. Materials (Basel). 13:33442020. View Article : Google Scholar : PubMed/NCBI

10 

Safina I, Alghazali KM, Childress L, Griffin C, Hashoosh A, Kannarpady G, Watanabe F, Bourdo SE, Dings RPM, Biris AS and Vang KB: Dendritic cell biocompatibility of ether-based urethane films. J Appl Toxicol. 41:1456–1466. 2021. View Article : Google Scholar : PubMed/NCBI

11 

Ssemakalu CC, Ubomba-Jaswa E, Motaung KSCM and Pillay M: Solar inactivated Vibrio cholerae induces maturation of JAWS II dendritic cell line in vitro. J Water Health. 18:494–504. 2020. View Article : Google Scholar : PubMed/NCBI

12 

Baldin AV, Savvateeva LV, Bazhin AV and Zamyatnin AA Jr: Dendritic Cells in anticancer vaccination: Rationale for ex vivo loading or in vivo targeting. Cancers (Basel). 12:5902020. View Article : Google Scholar : PubMed/NCBI

13 

Wculek SK, Cueto FJ, Mujal AM, Melero I, Krummel MF and Sancho D: Dendritic cells in cancer immunology and immunotherapy. Nat Rev Immunol. 20:7–24. 2020. View Article : Google Scholar : PubMed/NCBI

14 

Salah A, Wang H, Li Y, Ji M, Ou WB, Qi N and Wu Y: Insights into dendritic cells in cancer immunotherapy: From bench to clinical applications. Front Cell Dev Biol. 9:6865442021. View Article : Google Scholar : PubMed/NCBI

15 

Lutz MB and Schuler G: Immature, semi-mature and fully mature dendritic cells: Which signals induce tolerance or immunity? Trends Immunol. 23:445–449. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Dalod M, Chelbi R, Malissen B and Lawrence T: Dendritic cell maturation: Functional specialization through signaling specificity and transcriptional programming. EMBO J. 33:1104–1116. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Yi HJ and Lu GX: Adherent and non-adherent dendritic cells are equivalently qualified in GM-CSF, IL-4 and TNF-α culture system. Cell Immunol. 277:44–48. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Wang J, Dai X, Hsu C, Ming C, He Y, Zhang J, Wei L, Zhou P, Wang CY, Yang J and Gong N: Discrimination of the heterogeneity of bone marrow-derived dendritic cells. Mol Med Rep. 16:6787–6793. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Machcińska M, Kotur M, Jankowska A, Maruszewska-Cheruiyot M, Łaski A, Kotkowska Z, Bocian K and Korczak-Kowalska G: Cyclosporine A, in contrast to rapamycin, affects the ability of dendritic cells to induce immune tolerance mechanisms. Arch Immunol Ther Exp (Warsz). 69:272021. View Article : Google Scholar : PubMed/NCBI

20 

Aksoy E, Saveanu L and Manoury B: The isoform selective roles of PI3Ks in dendritic cell biology and function. Front Immunol. 9:25742018. View Article : Google Scholar : PubMed/NCBI

21 

Datler H, Vogel A, Kerndl M, Baumgartinger C, Musiejovsky L, Makivic N, Frech S, Niederreiter B, Haider T, Pühringer M, et al: PI3K activity in dendritic cells exerts paradoxical effects during autoimmune inflammation. Mol Immunol. 111:32–42. 2019. View Article : Google Scholar : PubMed/NCBI

22 

Bhattacharyya S, Sen P, Wallet M, Long B, Baldwin AS Jr and Tisch R: Immunoregulation of dendritic cells by IL-10 is mediated through suppression of the PI3K/Akt pathway and of IkappaB kinase activity. Blood. 104:1100–1109. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Cahill CM, Rogers JT and Walker WA: The role of phosphoinositide 3-kinase signaling in intestinal inflammation. J Signal Transduct. 2012:3584762012. View Article : Google Scholar : PubMed/NCBI

24 

Hu J and Wan Y: Tolerogenic dendritic cells and their potential applications. Immunology. 132:307–314. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Svajger U and Rozman P: Tolerogenic dendritic cells: Molecular and cellular mechanisms in transplantation. J Leukoc Biol. 95:53–69. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Langenkamp A, Messi M, Lanzavecchia A and Sallusto F: Kinetics of dendritic cell activation: Impact on priming of TH1, TH2 and nonpolarized T cells. Nat Immunol. 1:311–316. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Abdi K, Singh NJ and Matzinger P: Lipopolysaccharide-activated dendritic cells: ‘Exhausted’ or alert and waiting? J Immunol. 188:5981–5989. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Pittenger MF, Discher DE, Péault BM, Phinney DG, Hare JM and Caplan AI: Mesenchymal stem cell perspective: Cell biology to clinical progress. NPJ Regen Med. 4:222019. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Maruszewska‑Cheruiyot M, Machcińska M, Kierasińska M and Donskow‑Lysoniewska K: Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncol Lett 25: 93, 2023.
APA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., & Donskow‑Lysoniewska, K. (2023). Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncology Letters, 25, 93. https://doi.org/10.3892/ol.2023.13680
MLA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25.3 (2023): 93.
Chicago
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25, no. 3 (2023): 93. https://doi.org/10.3892/ol.2023.13680
Copy and paste a formatted citation
x
Spandidos Publications style
Maruszewska‑Cheruiyot M, Machcińska M, Kierasińska M and Donskow‑Lysoniewska K: Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncol Lett 25: 93, 2023.
APA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., & Donskow‑Lysoniewska, K. (2023). Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncology Letters, 25, 93. https://doi.org/10.3892/ol.2023.13680
MLA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25.3 (2023): 93.
Chicago
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25, no. 3 (2023): 93. https://doi.org/10.3892/ol.2023.13680
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team