Heterogeneity of adherent and non‑adherent JAWS II dendritic cells

  • Authors:
    • Marta Maruszewska‑Cheruiyot
    • Maja Machcińska
    • Magdalena Kierasińska
    • Katarzyna Donskow‑Lysoniewska
  • View Affiliations

  • Published online on: January 25, 2023     https://doi.org/10.3892/ol.2023.13680
  • Article Number: 93
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Abstract

Dendritic cells (DCs) are crucial in the development of immune responses. DC JAWS II is a murine cell line frequently used in DC studies. These cells are grown in two cell fractions: Adherent and non‑adherent. The present study aimed to compare these two fractions in both immature and lipopolysaccharide (LPS)‑activated JAWS II cells. The present study analysed the condition, phenotype, antigen uptake capability, signalling properties and the influence on the activity of T cells using flow cytometry, mixed cell reaction and ELISA methods. Adherent immature JAWS II cells exhibited increased endocytosis and decreased activation of the Pi3K signalling pathway. After LPS activation, adherent JAWS II cells exhibited increased expression levels of CD80 and CD86 costimulatory molecules, increased endocytosis and an elevated ability to induce T cell proliferation, compared with non‑adherent cells. These results demonstrated that the two fractions of JAWS II adherent and non‑adherent cells exhibited different properties and this should be taken into account in the planning of research.
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March-2023
Volume 25 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Maruszewska‑Cheruiyot M, Machcińska M, Kierasińska M and Donskow‑Lysoniewska K: Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncol Lett 25: 93, 2023
APA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., & Donskow‑Lysoniewska, K. (2023). Heterogeneity of adherent and non‑adherent JAWS II dendritic cells. Oncology Letters, 25, 93. https://doi.org/10.3892/ol.2023.13680
MLA
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25.3 (2023): 93.
Chicago
Maruszewska‑Cheruiyot, M., Machcińska, M., Kierasińska, M., Donskow‑Lysoniewska, K."Heterogeneity of adherent and non‑adherent JAWS II dendritic cells". Oncology Letters 25, no. 3 (2023): 93. https://doi.org/10.3892/ol.2023.13680