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Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling

  • Authors:
    • Tieli Wu
    • Dandan Zhu
    • Xingyi Wu
    • Ningning Zhang
    • Qingyuan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang 150000, P.R. China, Department of Medical Oncology, Daqing Oilfield General Hospital, Daqing, Heilongjiang 163000, P.R. China, Department of Internal Medicine, Qiqihar First Factory Hospital, Qiqihar, Heilongjiang 161000, P.R. China, School of Basic Medicine, Qiqihar Medical University, Qiqihar, Heilongjiang 161000, P.R. China
    Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 119
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    Published online on: February 7, 2023
       https://doi.org/10.3892/ol.2023.13705
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Abstract

Acquired resistance to Taxol (TAX) contributes to clinical treatment failure and significantly reduces the survival rate of patients. The present study aimed to explore the effects of exosomal microRNA (miR)‑187‑5p on TAX resistance in breast cancer cells and its underlying mechanisms. Exosomes were isolated from MCF‑7 and TAX‑resistant MCF‑7/TAX cells, and the miR‑187‑5p and miR‑106a‑3p levels of the cells and exosomes were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Next, MCF‑7 cells were treated with TAX for 48 h and either treated with exosomes or transfected with miR‑187‑5p mimics. Cell viability, apoptosis, migration, invasion and colony formation were determined using Cell Counting Kit‑8, flow cytometry, Transwell and colony formation assays, and the expression levels of associated genes and proteins were detected by RT‑qPCR and western blotting, respectively. Finally, a dual‑luciferase reporter gene assay was performed to confirm the target of miR‑187‑5p. The results showed that miR‑187‑5p expression levels increased significantly in TAX‑resistant MCF‑7 cells and exosomes compared with normal MCF‑7 cells and exosomes (P<0.05). However, miR‑106a‑3p was not detected in the cells or exosomes. Therefore, miR‑187‑5p was selected for subsequent experiments. A series of cell assays showed that TAX inhibited the viability, migration, invasion and colony formation of MCF‑7 cells and promoted their apoptosis; however, these changes were reversed by resistant cell exosomes and miR‑187‑5p mimics. Additionally, TAX significantly upregulated ABCD2 and downregulated β‑catenin, c‑Myc and cyclin D1, whereas resistant exosomes and miR‑187‑5p mimics reversed the TAX‑induced changes in expression. Finally, ABCD2 was confirmed to directly bind with miR‑187‑5p. It may be concluded that TAX‑resistant cell‑derived exosomes delivering miR‑187‑5p may affect the growth of TAX‑induced breast cancer cells by targeting ABCD2 and c‑Myc/Wnt/β‑catenin signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Wu T, Zhu D, Wu X, Zhang N and Zhang Q: Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling. Oncol Lett 25: 119, 2023.
APA
Wu, T., Zhu, D., Wu, X., Zhang, N., & Zhang, Q. (2023). Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling. Oncology Letters, 25, 119. https://doi.org/10.3892/ol.2023.13705
MLA
Wu, T., Zhu, D., Wu, X., Zhang, N., Zhang, Q."Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling". Oncology Letters 25.3 (2023): 119.
Chicago
Wu, T., Zhu, D., Wu, X., Zhang, N., Zhang, Q."Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling". Oncology Letters 25, no. 3 (2023): 119. https://doi.org/10.3892/ol.2023.13705
Copy and paste a formatted citation
x
Spandidos Publications style
Wu T, Zhu D, Wu X, Zhang N and Zhang Q: Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling. Oncol Lett 25: 119, 2023.
APA
Wu, T., Zhu, D., Wu, X., Zhang, N., & Zhang, Q. (2023). Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling. Oncology Letters, 25, 119. https://doi.org/10.3892/ol.2023.13705
MLA
Wu, T., Zhu, D., Wu, X., Zhang, N., Zhang, Q."Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling". Oncology Letters 25.3 (2023): 119.
Chicago
Wu, T., Zhu, D., Wu, X., Zhang, N., Zhang, Q."Taxol‑resistant breast cancer cell‑derived exosome‑delivered miR‑187‑5p regulates the growth of breast cancer cells via ABCD2 and Wnt/β‑catenin signaling". Oncology Letters 25, no. 3 (2023): 119. https://doi.org/10.3892/ol.2023.13705
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