Open Access

Effects of Helicobacter pylori on the expression of the FTO gene and its biological role in gastric cancer

  • Authors:
    • Shuqin Gui
    • Qinrong Wang
    • Liya Bao
    • Xiaofeng He
    • Zhengrong Wang
    • Linlin Liu
    • Lixia Wu
    • Yan Zhao
    • Jianjiang Zhou
    • Yuan Xie
  • View Affiliations

  • Published online on: February 28, 2023     https://doi.org/10.3892/ol.2023.13729
  • Article Number: 143
  • Copyright: © Gui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Helicobacter pylori (Hp) is a primary risk factor for gastric cancer. The fat mass and obesity‑associated (FTO) gene is associated with the development and progression of various cancer types such as glioma, leukemia, breast cancer and colorectal cancer. The aim of the present study was to investigate the effect of Hp infection on the expression of FTO and its roles in gastric cancer. It was found that the expression levels of both FTO mRNA and protein were significantly increased in Hp‑infected human gastric mucosal epithelial cells and Mongolian gerbil gastric tissues. The expression of FTO in gastric cancer tissues was higher than that in para‑cancer tissues. Data from The Cancer Genome Atlas demonstrated that FTO expression in gastric cancer tissues was significantly higher than that in normal tissues. Patient survival rate was significantly decreased in patients with high expression levels of FTO. It was also demonstrated that FTO expression was associated with several pathological parameters, such as tumor stage, metastasis stage and the American Joint Committee on Cancer stage. The FTO gene was positively correlated with 16,601 genes in gastric cancer and negatively correlated with 3,623 genes. Gene Ontology enrichment analysis demonstrated that FTO was significantly enriched in the regulation of gene expression and oxidative RNA demethylase activity, and it was associated with components such as the RNA N6‑methyladenosine methyltransferase complex and nuclear speckle. In addition, knockdown of the FTO gene inhibited the migration and invasion of Hp‑infected cells. In conclusion, the data suggests that Hp infection leads to upregulation of the FTO gene, which may be related to patient survival rate, tumor staging and other pathological parameters of patients with gastric cancer. It also suggests that FTO promotes proliferation and migration of gastric cancer cells, which may be involved in the pathogenesis of Hp‑induced gastric cancer.
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April-2023
Volume 25 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Gui S, Wang Q, Bao L, He X, Wang Z, Liu L, Wu L, Zhao Y, Zhou J, Xie Y, Xie Y, et al: Effects of Helicobacter pylori on the expression of the FTO gene and its biological role in gastric cancer. Oncol Lett 25: 143, 2023
APA
Gui, S., Wang, Q., Bao, L., He, X., Wang, Z., Liu, L. ... Xie, Y. (2023). Effects of Helicobacter pylori on the expression of the FTO gene and its biological role in gastric cancer. Oncology Letters, 25, 143. https://doi.org/10.3892/ol.2023.13729
MLA
Gui, S., Wang, Q., Bao, L., He, X., Wang, Z., Liu, L., Wu, L., Zhao, Y., Zhou, J., Xie, Y."Effects of Helicobacter pylori on the expression of the FTO gene and its biological role in gastric cancer". Oncology Letters 25.4 (2023): 143.
Chicago
Gui, S., Wang, Q., Bao, L., He, X., Wang, Z., Liu, L., Wu, L., Zhao, Y., Zhou, J., Xie, Y."Effects of Helicobacter pylori on the expression of the FTO gene and its biological role in gastric cancer". Oncology Letters 25, no. 4 (2023): 143. https://doi.org/10.3892/ol.2023.13729