Open Access

Urinary endogenous peptides as biomarkers for prostate cancer

  • Authors:
    • Cristine De Souza Dutra
    • Deborah Da Cruz Schafhauser
    • Mariana Hentz
    • Nicole Raupp Mayer
    • Raiane Medeiros Pinheiro
    • Gabriele Baierle
    • Djulia Rafaella Kist
    • Danielly Joani Bullé
    • Rodrigo Cattelan Donaduzzi
    • Marcus Falcão Bohmgahren
    • Arnaldo Zaha
    • Henrique Bunselmeyer Ferreira
    • Lia Gonçalves Possuelo
    • Karina Mariante Monteiro
  • View Affiliations

  • Published online on: March 14, 2023     https://doi.org/10.3892/ol.2023.13759
  • Article Number: 173
  • Copyright: © De Souza Dutra et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Prostate cancer (PCa) is one of the most prevalent types of cancer in men worldwide; however, the main diagnostic tests available for PCa have limitations and a biopsy is required for histopathological confirmation of the disease. Prostate‑specific antigen (PSA) is the main biomarker used for the early detection of PCa, but an elevated serum concentration is not cancer‑specific. Therefore, there is a need for the discovery of new non‑invasive biomarkers that can accurately diagnose PCa. The present study used trichloroacetic acid‑induced protein precipitation and liquid chromatography‑mass spectrometry to profile endogenous peptides in urine samples from patients with PCa (n=33), benign prostatic hyperplasia (n=25) and healthy individuals (n=28). Receiver operating characteristic curve analysis was performed to evaluate the diagnostic performance of urinary peptides. In addition, Proteasix tool was used for in silico prediction of protease cleavage sites. Five urinary peptides derived from uromodulin were revealed to be significantly altered between the study groups, all of which were less abundant in the PCa group. This peptide panel showed a high potential to discriminate between the study groups, resulting in area under the curve (AUC) values between 0.788 and 0.951. In addition, urinary peptides outperformed PSA in discriminating between malignant and benign prostate conditions (AUC=0.847), showing high sensitivity (81.82%) and specificity (88%). From in silico analyses, the proteases HTRA2, KLK3, KLK4, KLK14 and MMP25 were identified as potentially involved in the degradation of uromodulin peptides in the urine of patients with PCa. In conclusion, the present study allowed the identification of urinary peptides with potential for use as non‑invasive biomarkers in PCa diagnosis.
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April-2023
Volume 25 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
De Souza Dutra C, Da Cruz Schafhauser D, Hentz M, Mayer NR, Pinheiro RM, Baierle G, Kist DR, Bullé DJ, Donaduzzi RC, Bohmgahren MF, Bohmgahren MF, et al: Urinary endogenous peptides as biomarkers for prostate cancer. Oncol Lett 25: 173, 2023
APA
De Souza Dutra, C., Da Cruz Schafhauser, D., Hentz, M., Mayer, N.R., Pinheiro, R.M., Baierle, G. ... Monteiro, K.M. (2023). Urinary endogenous peptides as biomarkers for prostate cancer. Oncology Letters, 25, 173. https://doi.org/10.3892/ol.2023.13759
MLA
De Souza Dutra, C., Da Cruz Schafhauser, D., Hentz, M., Mayer, N. R., Pinheiro, R. M., Baierle, G., Kist, D. R., Bullé, D. J., Donaduzzi, R. C., Bohmgahren, M. F., Zaha, A., Ferreira, H. B., Possuelo, L. G., Monteiro, K. M."Urinary endogenous peptides as biomarkers for prostate cancer". Oncology Letters 25.4 (2023): 173.
Chicago
De Souza Dutra, C., Da Cruz Schafhauser, D., Hentz, M., Mayer, N. R., Pinheiro, R. M., Baierle, G., Kist, D. R., Bullé, D. J., Donaduzzi, R. C., Bohmgahren, M. F., Zaha, A., Ferreira, H. B., Possuelo, L. G., Monteiro, K. M."Urinary endogenous peptides as biomarkers for prostate cancer". Oncology Letters 25, no. 4 (2023): 173. https://doi.org/10.3892/ol.2023.13759