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HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration

  • Authors:
    • Xia Gao
    • Lina Yang
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology, Heping Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Gynecology, The 521 Hospital of Norinco Group, Xi'an, Shaanxi 710065, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 186
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    Published online on: March 24, 2023
       https://doi.org/10.3892/ol.2023.13772
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Abstract

Hepatitis B X‑interacting protein (HBXIP) and four and a half LIM domain 2 (FHL2) have been reported to serve as independent biomarkers for cervical cancer. The present study evaluated the effects of HBXIP on cervical cancer in terms of its cellular malignant characteristics. Reverse transcription‑quantitative PCR and western blotting were used to assess the mRNA and protein expression levels of HBXIP and FHL2 in the human endocervical epithelial End1/E6E7 cell line and the cervical cancer HeLa, CaSki, C33A and SiHa cell lines. After knocking down HBXIP expression by transfection of small interfering RNAs targeting HBXIP, cell cycle progression was assessed using flow cytometry with PI staining. Cell Counting Kit‑8, 5‑ethynyl‑2'‑deoxyuridine staining, wound healing and Transwell assays were used to assess cell proliferation, migration and invasion, respectively. Furthermore, co‑immunoprecipitation assay was used to evaluate the potential binding relationship between HBXIP and FHL2. Western blotting was used for the analysis of HBXIP and FHL2, cell cycle‑associated proteins, including cyclin D1 and cyclin D2, metastasis‑associated proteins, including MMP2 and MMP9, and Wnt/β‑catenin signaling‑associated proteins, including β‑catenin and c‑Myc. Both HBXIP and FHL2 were found to be highly expressed in cervical cancer cells compared with that in the human endocervical epithelial cell line. HBXIP knockdown suppressed the proliferation, invasion and migration of HeLa cells, but promoted cell cycle arrest at the G0/G1 phase. HBXIP was demonstrated to interact with FHL2, and HBXIP knockdown also inhibited FHL2 mRNA and protein expression. By contrast, FHL2 overexpression reversed the inhibitory effects of HBXIP knockdown on the malignant characteristics of cervical cancer cells. Furthermore, HBXIP knockdown blocked the Wnt/β‑catenin signaling pathway in HeLa cells, which was also partially reversed by FHL2 overexpression; the decreased β‑catenin and c‑Myc expression caused by HBXIP knockdown was increased again after FHL2 was overexpressed. In conclusion, these results suggest that HBXIP knockdown suppressed the malignant characteristics of cervical cancer cells through the downregulation of FHL2 expression, indicating a promising insight into the therapeutic target of cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Gao X and Yang L: HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration. Oncol Lett 25: 186, 2023.
APA
Gao, X., & Yang, L. (2023). HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration. Oncology Letters, 25, 186. https://doi.org/10.3892/ol.2023.13772
MLA
Gao, X., Yang, L."HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration". Oncology Letters 25.5 (2023): 186.
Chicago
Gao, X., Yang, L."HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration". Oncology Letters 25, no. 5 (2023): 186. https://doi.org/10.3892/ol.2023.13772
Copy and paste a formatted citation
x
Spandidos Publications style
Gao X and Yang L: HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration. Oncol Lett 25: 186, 2023.
APA
Gao, X., & Yang, L. (2023). HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration. Oncology Letters, 25, 186. https://doi.org/10.3892/ol.2023.13772
MLA
Gao, X., Yang, L."HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration". Oncology Letters 25.5 (2023): 186.
Chicago
Gao, X., Yang, L."HBXIP knockdown inhibits FHL2 to promote cycle arrest and suppress cervical cancer cell proliferation, invasion and migration". Oncology Letters 25, no. 5 (2023): 186. https://doi.org/10.3892/ol.2023.13772
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