Competing endogenous RNA analysis identified lncRNA DSCR9 as a novel prognostic biomarker associated with metastasis and tumor microenvironment in renal cell carcinoma

Liu,Ying; Luo,Jian; Zeng,Jing; Liu,Weipeng; Fu,Bin; Xiong,Jing

doi:10.3892/ol.2023.13876

Competing endogenous RNA analysis identified lncRNA DSCR9 as a novel prognostic biomarker associated with metastasis and tumor microenvironment in renal cell carcinoma

Authors:
- Ying Liu
- Jian Luo
- Jing Zeng
- Weipeng Liu
- Bin Fu
- Jing Xiong
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Affiliations: Department of Preventive Medicine, School of Public Health, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Laboratory Medicine, The First People's Hospital of Yichang, The People's Hospital of China Three Gorges University, Yichang, Hubei 443000, P.R. China, Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330000, P.R. China
Published online on: May 22, 2023 https://doi.org/10.3892/ol.2023.13876
Article Number: 290
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Distant metastasis is the main cause of death in patients with clear cell renal carcinoma (ccRCC). The dysregulation of the tumor microenvironment is responsible for tumorigenesis and metastasis in ccRCC. The role of long non‑coding RNAs in the tumor immune of ccRCC remains unclear. The present study screened differentially expressed protein‑coding genes and non‑coding genes between ccRCC and normal tissues based on three datasets. The commonly deregulated genes were used to identify distant metastasis‑related long non‑coding RNAs (lncRNAs) and prognostic lncRNAs. Pearson correlation analysis was used to identify immune‑related lncRNAs. A competing endogenous RNA network was constructed and hub lncRNAs were identified. A total of 1650 coding genes, 821 lncRNAs and 62 miRNAs were commonly deregulated in the three datasets. A total of 408 lncRNAs associated with the overall survival of patients with ccRCC were identified. Among them, 82 lncRNAs were distant metastasis‑related. Further analysis identified 52 lncRNAs associated with the immune pathway. Functional analyses concordantly demonstrated the role of the 52 lncRNAs in metastasis and tumor immunology. The ceRNA network analysis indicated lncRNA DSCR9 as the key lncRNA regulator. Univariate and multivariate analysis in two independent cohorts validated that DSCR9 could be an independent risk factor for the progression‑free survival of patients with ccRCC. Further analyses indicated that DSCR9 might be associated with the immunotherapeutic response. reverse transcription‑quantitative PCR demonstrated that the RNA expression level of DSCR9 was upregulated in ccRCC compared with normal kidney samples. The present study demonstrated the potential of LncRNA DSCR9 in assessing the prognosis and developing future immunotherapy for patients with metastatic ccRCC.

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