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CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process

  • Authors:
    • Yang Zhang
    • Lianghua Luo
    • Chengchao Fu
    • Wang Hu
    • Yong Li
    • Jianbo Xiong
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 291
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    Published online on: May 22, 2023
       https://doi.org/10.3892/ol.2023.13877
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Abstract

Liver cancer (LC) is a malignant tumour that is associated with high mortality rates worldwide. Cell division cycle 23 (CDC23) acts as an oncogene in papillary thyroid cancer. In addition, epithelial‑mesenchymal transition (EMT) is frequently involved in the malignant metastasis of various cancer types. Therefore, we hypothesized that CDC23 may regulate the malignant biological behaviours of LC cells through EMT. Proliferation, colony formation and Transwell assays, western blotting and xenograft experiments were performed. The results of the present study showed that CDC23 was highly expressed in LC cell lines. In addition, it was found via multiple in vitro assays that CDC23 knockdown reduced the proliferation, migration and invasion of LC cell lines. Finally, an in vivo study confirmed that CDC23 knockdown inhibited the growth of xenograft LC in nude mice. More importantly, the changes in the levels of EMT‑related marker proteins were analysed in the sh‑CDC23 group compared with the sh‑NC group of cells and xenografts. E‑cadherin was upregulated, and N‑cadherin and vimentin were significantly downregulated after CDC23 silencing. Taken together, these results revealed that the knockdown of CDC23 inhibits the progression of LC by regulating EMT and that CDC23 may be a novel therapeutic target for LC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Luo L, Fu C, Hu W, Li Y and Xiong J: CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. Oncol Lett 26: 291, 2023.
APA
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., & Xiong, J. (2023). CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. Oncology Letters, 26, 291. https://doi.org/10.3892/ol.2023.13877
MLA
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., Xiong, J."CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process". Oncology Letters 26.1 (2023): 291.
Chicago
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., Xiong, J."CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process". Oncology Letters 26, no. 1 (2023): 291. https://doi.org/10.3892/ol.2023.13877
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Luo L, Fu C, Hu W, Li Y and Xiong J: CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. Oncol Lett 26: 291, 2023.
APA
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., & Xiong, J. (2023). CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process. Oncology Letters, 26, 291. https://doi.org/10.3892/ol.2023.13877
MLA
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., Xiong, J."CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process". Oncology Letters 26.1 (2023): 291.
Chicago
Zhang, Y., Luo, L., Fu, C., Hu, W., Li, Y., Xiong, J."CDC23 knockdown suppresses the proliferation, migration and invasion of liver cancer via the EMT process". Oncology Letters 26, no. 1 (2023): 291. https://doi.org/10.3892/ol.2023.13877
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