Open Access

Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition

  • Authors:
    • Xue Tian
    • Jiaan Xu
    • Yonghua Ye
    • Xiujuan Xiao
    • Li Yan
    • Shihui Yu
    • Jianyong Cai
    • Quan Du
    • Xiaoqiao Dong
    • Li Zhou
    • Letian Shan
    • Qiang Yuan
  • View Affiliations

  • Published online on: May 23, 2023     https://doi.org/10.3892/ol.2023.13880
  • Article Number: 294
  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The bioactive extract of green tea, theabrownin (TB), is known to exhibit pro‑apoptotic and antitumor effects on non‑small cell lung cancer (NSCLC). Gallic acid (GA) is a crucial component of TB; however, its mechanism of action in NSCLC has been rarely studied. To date, little attention has been paid to the anti‑NSCLC activity of GA. Therefore, the present study investigated the effects of GA in vivo and in vitro. Cell Counting Kit (CCK)‑8 assay, DAPI staining and flow cytometry, wound‑healing assay and western blotting were used to assess cell viability, apoptosis, migration and protein expression, respectively. In addition, a xenograft model was generated, and TUNEL assay and immunohistochemistry analysis were performed. The CCK‑8 data showed that the viability of H1299 cells was significantly inhibited by GA in a dose‑ and time‑dependent manner. DAPI staining, Annexin‑V/PI staining and wound‑healing data showed that GA exerted pro‑apoptotic and anti‑migratory effects on H1299 cells in a dose‑dependent manner. Furthermore, the results of western blotting showed that GA significantly upregulated the levels of pro‑apoptotic proteins [cleaved (c‑)PARP, c‑caspase8, c‑caspase‑9 and the ratio of γ‑H2A.X/H2A.X]. In vivo data confirmed the antitumor effect of GA through apoptosis induction in an autophagy‑dependent manner. In conclusion, the present study confirmed the anti‑proliferative, pro‑apoptotic and anti‑migratory effects of GA against NSCLC in vitro and in vivo, providing considerable evidence for its potential as a novel candidate for the treatment of NSCLC.
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July-2023
Volume 26 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Tian X, Xu J, Ye Y, Xiao X, Yan L, Yu S, Cai J, Du Q, Dong X, Zhou L, Zhou L, et al: Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition. Oncol Lett 26: 294, 2023
APA
Tian, X., Xu, J., Ye, Y., Xiao, X., Yan, L., Yu, S. ... Yuan, Q. (2023). Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition. Oncology Letters, 26, 294. https://doi.org/10.3892/ol.2023.13880
MLA
Tian, X., Xu, J., Ye, Y., Xiao, X., Yan, L., Yu, S., Cai, J., Du, Q., Dong, X., Zhou, L., Shan, L., Yuan, Q."Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition". Oncology Letters 26.1 (2023): 294.
Chicago
Tian, X., Xu, J., Ye, Y., Xiao, X., Yan, L., Yu, S., Cai, J., Du, Q., Dong, X., Zhou, L., Shan, L., Yuan, Q."Gallic acid in theabrownin suppresses cell proliferation and migration in non‑small cell lung carcinoma via autophagy inhibition". Oncology Letters 26, no. 1 (2023): 294. https://doi.org/10.3892/ol.2023.13880