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Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma

  • Authors:
    • Jiali Li
    • Lili Lan
    • Yuru Xu
    • Shenghui Liu
    • Meng Liu
    • Guobin Hu
    • Ganxun Wu
    • Yan Zhao
    • Jian Shi
    • Jingtian Wang
    • Yixin Sun
    • Zhanlong Wang
    • Ruili Zhao
  • View Affiliations / Copyright

    Affiliations: Research Center, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Otolaryngology Head and Neck Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 310
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    Published online on: June 2, 2023
       https://doi.org/10.3892/ol.2023.13896
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Abstract

The aim of the present study was to evaluate the expression of TRAF2‑ and NCK‑interacting kinase (TNIK) and the levels of the active form of TNIK, phosphorylated (p)‑TNIK, in papillary thyroid carcinoma (PTC), and to identify and compare the levels of TNIK and p‑TNIK among PTC, benign thyroid tumors and normal tissues. The levels of TNIK and p‑TNIK were examined by reverse transcription‑quantitative (RT‑q)PCR and immunohistochemical analysis (IHC) in PTC, benign thyroid tumors and normal tissues, and their association with clinicopathological features was evaluated. First, analysis of the Gene Expression Profiling Interactive Analysis and The Cancer Genome Atlas datasets suggested that the mRNA expression of TNIK was markedly increased in PTC tissues compared with that in normal tissues. RT‑qPCR analyses then indicated that the relative mRNA expression of TNIK in PTC tissues was 4.47±6.16, which was significantly higher than that in adjacent tissues 2.57±5.83. The IHC results suggested that the levels of TNIK and p‑TNIK in PTC tissues were markedly elevated compared with those in benign thyroid tumors and normal tissues. The levels of p‑TNIK in patients with PTC were significantly associated with extrathyroidal extension (χ2=4.199, P=0.040). Positive staining for TNIK was observed in 187 out of 202 (92.6%) cases in the cytoplasm, nucleus or cytomembrane of PTC cells. Among the 187 positive cases, cytoplasm expression was identified in 162 cases (86.6%), nuclear expression in 17 cases (9.1%) and cytomembrane expression in 8 cases (4.3%). Positive staining for p‑TNIK was observed in 179 out of 202 (88.6%) cases in the nuclei, cytoplasm or cytomembrane of PTC cells. In the 179 p‑TNIK‑positive cases, localization in the nuclei plus cytoplasm was identified in 142 cases (79.3%), nuclear localization in 9 cases (5.0%), presence in the cytoplasm in 21 cases (11.7%) and cytomembrane localization in 7 cases (3.9%). Both TNIK and p‑TNIK were upregulated in PTC tissues and p‑TNIK was significantly associated with extrathyroidal extension. It may act as a crucial oncogene to participate in PTC carcinogenesis and progression.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Lan L, Xu Y, Liu S, Liu M, Hu G, Wu G, Zhao Y, Shi J, Wang J, Wang J, et al: Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma. Oncol Lett 26: 310, 2023.
APA
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G. ... Zhao, R. (2023). Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma. Oncology Letters, 26, 310. https://doi.org/10.3892/ol.2023.13896
MLA
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G., Wu, G., Zhao, Y., Shi, J., Wang, J., Sun, Y., Wang, Z., Zhao, R."Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma". Oncology Letters 26.1 (2023): 310.
Chicago
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G., Wu, G., Zhao, Y., Shi, J., Wang, J., Sun, Y., Wang, Z., Zhao, R."Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma". Oncology Letters 26, no. 1 (2023): 310. https://doi.org/10.3892/ol.2023.13896
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Lan L, Xu Y, Liu S, Liu M, Hu G, Wu G, Zhao Y, Shi J, Wang J, Wang J, et al: Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma. Oncol Lett 26: 310, 2023.
APA
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G. ... Zhao, R. (2023). Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma. Oncology Letters, 26, 310. https://doi.org/10.3892/ol.2023.13896
MLA
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G., Wu, G., Zhao, Y., Shi, J., Wang, J., Sun, Y., Wang, Z., Zhao, R."Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma". Oncology Letters 26.1 (2023): 310.
Chicago
Li, J., Lan, L., Xu, Y., Liu, S., Liu, M., Hu, G., Wu, G., Zhao, Y., Shi, J., Wang, J., Sun, Y., Wang, Z., Zhao, R."Expression analysis of TRAF2‑ and NCK‑interacting protein kinase (TNIK) and phosphorylated TNIK in papillary thyroid carcinoma". Oncology Letters 26, no. 1 (2023): 310. https://doi.org/10.3892/ol.2023.13896
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