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Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion

  • Authors:
    • Jiaqi Zhu
    • Qi Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology, Beilun People's Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang University, Ningbo, Zhejiang 315826, P.R. China, Department of Obstetrics, Beilun People's Hospital, Beilun Branch of the First Affiliated Hospital, School of Medicine, Zhejiang University, Ningbo, Zhejiang 315826, P.R. China
    Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 335
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    Published online on: June 20, 2023
       https://doi.org/10.3892/ol.2023.13921
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Abstract

Claudin‑4, a member of the claudin multigene family, participates in events associated with mesenchymal‑like activity of cancerous cells. Claudin‑4 expression is upregulated in cervical cancer tissue compared with that in adjoining non‑neoplastic tissue. However, the mechanisms that regulate Claudin‑4 expression in cervical cancer are poorly understood. Moreover, whether Claudin‑4 contributes to the migration and invasion of cervical cancer cells remains unclear. By western blotting, reverse transcription‑qPCR, bioinformatics analysis, dual‑luciferase reporter assay, chromatin immunoprecipitation assay, wound healing assay and Transwell migration/invasion assay, the present study confirmed that Claudin‑4 was a downstream target of Twist1, a helix‑loop‑helix transcriptional factor, the activity of which has a positive correlation with Claudin‑4 expression. Mechanistically, Twist1 directly binds to Claudin‑4 promoter, resulting in the transactivation of expression. The depletion of the Twist1‑binding E‑Box1 domain on Claudin‑4 promoter via CRISPR‑Cas9 knockout system downregulates Claudin‑4 expression and suppresses the ability of cervical cancer cells to migrate and invade by elevating E‑cadherin levels and lowering N‑cadherin levels. Following activation by transforming growth factor‑β, Twist1 induces Claudin‑4 expression, thus enhancing migration and invasion of cervical cancer cells. In summary, the present data suggested that Claudin‑4 was a direct downstream target of Twist1 and served a critical role in promoting Twist1‑mediated cervical cancer cell migration and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J and Jiang Q: Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion. Oncol Lett 26: 335, 2023.
APA
Zhu, J., & Jiang, Q. (2023). Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion. Oncology Letters, 26, 335. https://doi.org/10.3892/ol.2023.13921
MLA
Zhu, J., Jiang, Q."Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion". Oncology Letters 26.2 (2023): 335.
Chicago
Zhu, J., Jiang, Q."Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion". Oncology Letters 26, no. 2 (2023): 335. https://doi.org/10.3892/ol.2023.13921
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J and Jiang Q: Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion. Oncol Lett 26: 335, 2023.
APA
Zhu, J., & Jiang, Q. (2023). Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion. Oncology Letters, 26, 335. https://doi.org/10.3892/ol.2023.13921
MLA
Zhu, J., Jiang, Q."Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion". Oncology Letters 26.2 (2023): 335.
Chicago
Zhu, J., Jiang, Q."Twist1‑mediated transcriptional activation of Claudin‑4 promotes cervical cancer cell migration and invasion". Oncology Letters 26, no. 2 (2023): 335. https://doi.org/10.3892/ol.2023.13921
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