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Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells

  • Authors:
    • Wen-Hui Hsieh
    • Shu-Wei Liao
    • Shun-Ming Chan
    • Jin-De Hou
    • Szu-Yuan Wu
    • Bing-Ying Ho
    • Kung-Yen Chen
    • Yu-Ting Tai
    • Hsu-Wei Fang
    • Chih-Yuan Fang
    • Se-Yi Chen
    • Jui-An Lin
  • View Affiliations / Copyright

    Affiliations: Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 10608, Taiwan, R.O.C., Department of Anesthesiology, Chi-Mei Medical Center, Tainan 71004, Taiwan, R.O.C., Department of Anesthesiology, Tri-Service General Hospital and National Defense Medical Center, Taipei 11490, Taiwan, R.O.C., Division of Anesthesiology, Hualien Armed Forces General Hospital, Hualien 97144, Taiwan, R.O.C., Center for Regional Anesthesia and Pain Medicine, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Primo Biotechnology Co., Ltd., Taipei 10480, Taiwan, R.O.C., Department of Anesthesiology, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Division of Oral and Maxillofacial Surgery, Department of Dentistry, Wan Fang Hospital, Taipei Medical University, Taipei 116, Taiwan, R.O.C., Department of Neurosurgery, Chung-Shan Medical University, Taichung 40201, Taiwan, R.O.C., Department of Anesthesiology, School of Medicine, National Defense Medical Center, Taipei 11490, Taiwan, R.O.C.
    Copyright: © Hsieh et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 346
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    Published online on: June 27, 2023
       https://doi.org/10.3892/ol.2023.13932
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Abstract

The effects of clinically relevant concentrations of lidocaine on epithelial‑mesenchymal transition (EMT) and associated lung cancer behaviors have rarely been investigated. The aim of the present study was to assess the impact of lidocaine on EMT and its related phenomena, including chemoresistance. Lung cancer cell lines (A549 and LLC.LG) were incubated with various concentrations of lidocaine, 5‑fluorouracil (5‑FU) or both to test their effects on cell viability. Subsequently, the effects of lidocaine on various cell behaviors were assessed in vitro and in vivo using Transwell migration, colony‑formation and anoikis‑resistant cell aggregation assays, and human tumor cell metastasis in a chorioallantoic membrane (CAM) model quantitated by PCR analysis. Prototypical EMT markers and their molecular switch were analyzed using western blotting. In addition, a conditioned metastasis pathway was generated through Ingenuity Pathway Analysis. Based on these measured proteins (slug, vimentin and E‑cadherin), the molecules involved and the alteration of genes associated with metastasis were predicted. Of note, clinically relevant concentrations of lidocaine did not affect lung cancer cell viability or alter the effects of 5‑FU on cell survival; however, at this dose range, lidocaine attenuated the 5‑FU‑induced inhibitory effect on cell migration and promoted EMT. The expression levels of vimentin and Slug were upregulated, whereas the expression of E‑cadherin was downregulated. EMT‑associated anoikis resistance was also induced by lidocaine administration. In addition, portions of the lower CAM with a dense distribution of blood vessels exhibited markedly increased Alu expression 24 h following the inoculation of lidocaine‑treated A549 cells on the upper CAM. Thus, at clinically relevant concentrations, lidocaine has the potential to aggravate cancer behaviors in non‑small cell lung cancer cells. The phenomena accompanying lidocaine‑aggravated migration and metastasis included altered prototypical EMT markers, anoikis‑resistant cell aggregation and attenuation of the 5‑FU‑induced inhibitory effect on cell migration.
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View References

1 

Dela Cruz CS, Tanoue LT and Matthay RA: Lung cancer: Epidemiology, etiology, and prevention. Clin Chest Med. 32:605–644. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Mountain CF: The international system for staging lung cancer. Semin Surg Oncol. 18:106–115. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Chen R, Manochakian R, James L, Azzouqa AG, Shi H, Zhang Y, Zhao Y, Zhou K and Lou Y: Emerging therapeutic agents for advanced non-small cell lung cancer. J Hematol Oncol. 13:582020. View Article : Google Scholar : PubMed/NCBI

4 

Noro R, Miyanaga A, Minegishi Y, Okano T, Seike M, Soeno C, Kataoka K, Matsuda K, Yoshimura A and Gemma A: Histone deacetylase inhibitor enhances sensitivity of non-small-cell lung cancer cells to 5-FU/S-1 via down-regulation of thymidylate synthase expression and up-regulation of p21(waf1/cip1) expression. Cancer Sci. 101:1424–1430. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Ettinger DS, Wood DE, Aggarwal C, Aisner DL, Akerley W, Bauman JR, Bharat A, Bruno DS, Chang JY, Chirieac LR, et al: NCCN guidelines insights: Non-small cell lung cancer, version 1.2020. J Natl Compr Canc Netw. 17:1464–1472. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Tan X, Zhang C, Gao W, Sun B, Jiang B and Song P: Overexpression of microRNA-124-5p sensitizes non-small cell lung cancer cells to treatment with 5-fluorouracil via AEG-1 regulation. Oncol Lett. 21:52021.PubMed/NCBI

7 

Chang A: Chemotherapy, chemoresistance and the changing treatment landscape for NSCLC. Lung Cancer. 71:3–10. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Tedore T: Regional anaesthesia and analgesia: Relationship to cancer recurrence and survival. Br J Anaesth. 115 (Suppl 2):ii34–ii45. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Xuan W, Hankin J, Zhao H, Yao S and Ma D: The potential benefits of the use of regional anesthesia in cancer patients. Int J Cancer. 137:2774–2784. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Piegeler T, Schläpfer M, Dull RO, Schwartz DE, Borgeat A, Minshall RD and Beck-Schimmer B: Clinically relevant concentrations of lidocaine and ropivacaine inhibit TNFα-induced invasion of lung adenocarcinoma cells in vitro by blocking the activation of Akt and focal adhesion kinase. Br J Anaesth. 115:784–791. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Piegeler T, Votta-Velis EG, Liu G, Place AT, Schwartz DE, Beck-Schimmer B, Minshall RD and Borgeat A: Antimetastatic potential of amide-linked local anesthetics: Inhibition of lung adenocarcinoma cell migration and inflammatory Src signaling independent of sodium channel blockade. Anesthesiology. 117:548–559. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Samarin MJ, Mohrien KM and Oliphant CS: Continuous intravenous antiarrhythmic agents in the intensive care unit: Strategies for safe and effective use of amiodarone, lidocaine, and procainamide. Crit Care Nurs Q. 38:329–344. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Kosharskyy B, Almonte W, Shaparin N, Pappagallo M and Smith H: Intravenous infusions in chronic pain management. Pain Physician. 16:231–249. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Keller D, Seamon J and Jones JS: BET 2: Usefulness of IV lidocaine in the treatment of renal colic. Emerg Med J. 33:825–826. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Nguyen NL, Kome AM, Lowe DK, Coyne P and Hawks KG: Intravenous lidocaine as an adjuvant for pain associated with sickle cell disease. J Pain Palliat Care Pharmacother. 29:359–364. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Williams DR and Stark RJ: Intravenous lignocaine (lidocaine) infusion for the treatment of chronic daily headache with substantial medication overuse. Cephalalgia. 23:963–971. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Dunn LK and Durieux ME: Perioperative use of intravenous lidocaine. Anesthesiology. 126:729–737. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Sun H and Sun Y: Lidocaine inhibits proliferation and metastasis of lung cancer cell via regulation of miR-539/EGFR axis. Artif Cells Nanomed Biotechnol. 47:2866–2874. 2019. View Article : Google Scholar : PubMed/NCBI

19 

Wang HW, Wang LY, Jiang L, Tian SM, Zhong TD and Fang XM: Amide-linked local anesthetics induce apoptosis in human non-small cell lung cancer. J Thorac Dis. 8:2748–2757. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Zhou Y, Du Q, Zhao Q, Zhang M, Qin X, Jiang Y and Luan Y: A heme-regulatable chemodynamic nanodrug harnessing transcription factor Bach1 against lung cancer metastasis. J Colloid Interface Sci. 610:698–708. 2022. View Article : Google Scholar : PubMed/NCBI

21 

Ning J, Cui X, Li N, Li N, Zhao B, Miao J and Lin Z: Activation of GRP78 ATPase suppresses A549 lung cancer cell migration by promoting ITGB4 degradation. Cell Adh Migr. 16:107–114. 2022. View Article : Google Scholar : PubMed/NCBI

22 

Ediriweera MK, Tennekoon KH, Samarakoon SR, Thabrew I and De Silva ED: Induction of apoptosis in MCF-7 breast cancer cells by sri lankan endemic mango (Mangifera zeylanica) fruit peel through oxidative stress and analysis of its phytochemical constituents. J Food Biochem. 41:e122942017. View Article : Google Scholar

23 

Orellana EA and Kasinski AL: Sulforhodamine B (SRB) assay in cell culture to investigate cell proliferation. Bio Protoc. 6:e19842016. View Article : Google Scholar : PubMed/NCBI

24 

Feoktistova M, Geserick P and Leverkus M: Crystal violet assay for determining viability of cultured cells. Cold Spring Harb Protoc. 2016.pdb.prot087379, 2016. View Article : Google Scholar

25 

Miyashita N, Enokido T, Horie M, Fukuda K, Urushiyama H, Strell C, Brunnström H, Micke P, Saito A and Nagase T: TGF-β-mediated epithelial-mesenchymal transition and tumor-promoting effects in CMT64 cells are reflected in the transcriptomic signature of human lung adenocarcinoma. Sci Rep. 11:223802021. View Article : Google Scholar : PubMed/NCBI

26 

Ho BY, Wu YM, Hsu YW, Hsu LC, Kuo YH, Chang KJ and Pan TM: Effects of Monascus-fermented rice extract on malignant cell-associated neovascularization and intravasation determined using the chicken embryo chorioallantoic membrane model. Integr Cancer Ther. 9:204–212. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Kim J, Yu W, Kovalski K and Ossowski L: Requirement for specific proteases in cancer cell intravasation as revealed by a novel semiquantitative PCR-based assay. Cell. 94:353–362. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Kariya Y, Kato K, Hayashizaki Y, Himeno S, Tarui S and Matsubara K: Revision of consensus sequence of human Alu repeats-a review. Gene. 53:1–10. 1987. View Article : Google Scholar : PubMed/NCBI

30 

Unahabhokha T, Chanvorachote P, Sritularak B, Kitsongsermthon J and Pongrakhananon V: Gigantol inhibits epithelial to mesenchymal process in human lung cancer cells. Evid Based Complement Alternat Med. 2016:45616742016. View Article : Google Scholar : PubMed/NCBI

31 

Usman S, Waseem NH, Nguyen TKN, Mohsin S, Jamal A, Teh MT and Waseem A: Vimentin is at the heart of epithelial mesenchymal transition (EMT) mediated metastasis. Cancers (Basel). 13:49852021. View Article : Google Scholar : PubMed/NCBI

32 

Mahmood MQ, Shukla SD, Ward C and Walters EH: The underappreciated role of epithelial mesenchymal transition in chronic obstructive pulmonary disease and its strong link to lung cancer. Biomolecules. 11:13942021. View Article : Google Scholar : PubMed/NCBI

33 

Zhu X, Chen L, Liu L and Niu X: EMT-mediated acquired EGFR-TKI resistance in NSCLC: Mechanisms and strategies. Front Oncol. 9:10442019. View Article : Google Scholar : PubMed/NCBI

34 

Ancel J, Dewolf M, Deslée G, Nawrocky-Raby B, Dalstein V, Gilles C and Polette M: Clinical impact of the epithelial-mesenchymal transition in lung cancer as a biomarker assisting in therapeutic decisions. Cells Tissues Organs. 211:91–109. 2022. View Article : Google Scholar : PubMed/NCBI

35 

Nieto MA, Huang RY, Jackson RA and Thiery JP: Emt: 2016. Cell. 166:21–45. 2016. View Article : Google Scholar : PubMed/NCBI

36 

Herroeder S, Pecher S, Schönherr ME, Kaulitz G, Hahnenkamp K, Friess H, Böttiger BW, Bauer H, Dijkgraaf MG, Durieux ME and Hollmann MW: Systemic lidocaine shortens length of hospital stay after colorectal surgery: A double-blinded, randomized, placebo-controlled trial. Ann Surg. 246:192–200. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Kaba A, Laurent SR, Detroz BJ, Sessler DI, Durieux ME, Lamy ML and Joris JL: Intravenous lidocaine infusion facilitates acute rehabilitation after laparoscopic colectomy. Anesthesiology. 106:11–18. 5–6. 2007. View Article : Google Scholar : PubMed/NCBI

38 

Mammoto T, Higashiyama S, Mukai M, Mammoto A, Ayaki M, Mashimo T, Hayashi Y, Kishi Y, Nakamura H and Akedo H: Infiltration anesthetic lidocaine inhibits cancer cell invasion by modulating ectodomain shedding of heparin-binding epidermal growth factor-like growth factor (HB-EGF). J Cell Physiol. 192:351–358. 2002. View Article : Google Scholar : PubMed/NCBI

39 

Mao L, Lin S and Lin J: The effects of anesthetics on tumor progression. Int J Physiol Pathophysiol Pharmacol. 5:1–10. 2013.PubMed/NCBI

40 

Koltai T: Voltage-gated sodium channel as a target for metastatic risk reduction with re-purposed drugs. F1000Res. 4:2972015. View Article : Google Scholar : PubMed/NCBI

41 

Elajnaf T, Baptista-Hon DT and Hales TG: Potent inactivation-dependent inhibition of adult and neonatal NaV1.5 channels by lidocaine and levobupivacaine. Anesth Analg. 127:650–660. 2018. View Article : Google Scholar : PubMed/NCBI

42 

Zhang L, Hu R, Cheng Y, Wu X, Xi S, Sun Y and Jiang H: Lidocaine inhibits the proliferation of lung cancer by regulating the expression of GOLT1A. Cell Prolif. 50:e123642017. View Article : Google Scholar : PubMed/NCBI

43 

Lin JA, Chen JH, Lee YW, Lin CS, Hsieh MH, Chang CC, Wong CS, Chen JJ, Yeh GC, Lin FY and Chen TL: Biphasic effect of curcumin on morphine tolerance: A preliminary evidence from cytokine/chemokine protein array analysis. Evid Based Complement Alternat Med. 2011:4521532011. View Article : Google Scholar : PubMed/NCBI

44 

Chan SM, Lin BF, Wong CS, Chuang WT, Chou YT and Wu ZF: Levobuipivacaine-induced dissemination of A549 lung cancer cells. Sci Rep. 7:86462017. View Article : Google Scholar : PubMed/NCBI

45 

Lokman NA, Elder ASF, Ricciardelli C and Oehler MK: Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis. Int J Mol Sci. 13:9959–9970. 2012. View Article : Google Scholar : PubMed/NCBI

46 

Merlos Rodrigo MA, Casar B, Michalkova H, Jimenez Jimenez AM, Heger Z and Adam V: Extending the applicability of in ovo and ex ovo chicken chorioallantoic membrane assays to study cytostatic activity in neuroblastoma cells. Front Oncol. 11:7073662021. View Article : Google Scholar : PubMed/NCBI

47 

Agrawal S, Chaugule S, More S, Rane G and Indap M: Methanolic extract of Euchelus asper exhibits in-ovo anti-angiogenic and in vitro anti-proliferative activities. Biol Res. 50:412017. View Article : Google Scholar : PubMed/NCBI

48 

Ribatti D: The chick embryo chorioallantoic membrane (CAM). A multifaceted experimental model. Mech Dev. 141:70–77. 2016. View Article : Google Scholar : PubMed/NCBI

49 

Valastyan S and Weinberg RA: Tumor metastasis: Molecular insights and evolving paradigms. Cell. 147:275–292. 2011. View Article : Google Scholar : PubMed/NCBI

50 

Pezzella F and Gatter KC: Evidence showing that tumors can grow without angiogenesis and can switch between angiogenic and nonangiogenic phenotypes. J Natl Cancer Inst. 108:djw0322016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hsieh W, Liao S, Chan S, Hou J, Wu S, Ho B, Chen K, Tai Y, Fang H, Fang C, Fang C, et al: Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells. Oncol Lett 26: 346, 2023.
APA
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B. ... Lin, J. (2023). Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells. Oncology Letters, 26, 346. https://doi.org/10.3892/ol.2023.13932
MLA
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B., Chen, K., Tai, Y., Fang, H., Fang, C., Chen, S., Lin, J."Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells". Oncology Letters 26.2 (2023): 346.
Chicago
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B., Chen, K., Tai, Y., Fang, H., Fang, C., Chen, S., Lin, J."Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells". Oncology Letters 26, no. 2 (2023): 346. https://doi.org/10.3892/ol.2023.13932
Copy and paste a formatted citation
x
Spandidos Publications style
Hsieh W, Liao S, Chan S, Hou J, Wu S, Ho B, Chen K, Tai Y, Fang H, Fang C, Fang C, et al: Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells. Oncol Lett 26: 346, 2023.
APA
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B. ... Lin, J. (2023). Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells. Oncology Letters, 26, 346. https://doi.org/10.3892/ol.2023.13932
MLA
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B., Chen, K., Tai, Y., Fang, H., Fang, C., Chen, S., Lin, J."Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells". Oncology Letters 26.2 (2023): 346.
Chicago
Hsieh, W., Liao, S., Chan, S., Hou, J., Wu, S., Ho, B., Chen, K., Tai, Y., Fang, H., Fang, C., Chen, S., Lin, J."Lidocaine induces epithelial‑mesenchymal transition and aggravates cancer behaviors in non‑small cell lung cancer A549 cells". Oncology Letters 26, no. 2 (2023): 346. https://doi.org/10.3892/ol.2023.13932
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