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Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma

  • Authors:
    • Jun Lu
    • Qianqian Zhang
    • Licai Mo
    • Weiying Chen
    • Linghong Mao
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    Affiliations: Department of Urology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou, Zhejiang 310000, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 395
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    Published online on: July 27, 2023
       https://doi.org/10.3892/ol.2023.13981
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Abstract

Clear cell renal cell carcinoma (ccRCC) is the most prominent subtype of renal cancer and E47‑like factors (ELFs) are important in tumorigenesis; however, the specific role of key ELFs in ccRCC remains unclear. The present study comprehensively analyzed RNA sequencing and clinical data from multiple databases, and identified differentially expressed ELFs (ELF3‑5) in ccRCC. The DNA promoter methylation, genetic variation and clinical significance of ELF3‑5 in ccRCC were analyzed using the cBioPortal and UALCAN databases. The association between ELF3‑5 and multiple immune cell infiltration was analyzed using Tumor Immune Estimation Resource. Subsequently, ELF4 was selected and its association with biological functions was assessed. Cell counting kit‑8 (CCK‑8), colony formation, Transwell, macrophage chemotaxis and polarization assays were conducted to validate the functions of ELF4. Notably, the mRNA expression levels of ELF4 were significantly upregulated in ccRCC, whereas ELF3 and ELF5 mRNA expression levels were significantly downregulated. Clinical significance analysis revealed that ELF4 showed a high clinical significance with tumor grade, clear cell type A and B subtypes, and incidence rates of amplification in genetic variation. Further analyses indicated that ELF4 may be involved in multiple immune cell differentiation. Additionally, cell experiments revealed that ELF4 inhibition downregulated 769‑P and 786‑O proliferation, migration and invasion. Knockdown of ELF4 in cancer cells also inhibited M2 macrophage polarization and chemotaxis towards 769‑P and 786‑O cells. Conclusively, the present findings indicated the clinical significance of ELF4 in ccRCC, and verified its key role in driving cell proliferation, migration and invasion, and promoting M2 macrophage polarization and chemotaxis in ccRCC.
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Copy and paste a formatted citation
Spandidos Publications style
Lu J, Zhang Q, Mo L, Chen W and Mao L: Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma. Oncol Lett 26: 395, 2023.
APA
Lu, J., Zhang, Q., Mo, L., Chen, W., & Mao, L. (2023). Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma. Oncology Letters, 26, 395. https://doi.org/10.3892/ol.2023.13981
MLA
Lu, J., Zhang, Q., Mo, L., Chen, W., Mao, L."Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma". Oncology Letters 26.3 (2023): 395.
Chicago
Lu, J., Zhang, Q., Mo, L., Chen, W., Mao, L."Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma". Oncology Letters 26, no. 3 (2023): 395. https://doi.org/10.3892/ol.2023.13981
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Zhang Q, Mo L, Chen W and Mao L: Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma. Oncol Lett 26: 395, 2023.
APA
Lu, J., Zhang, Q., Mo, L., Chen, W., & Mao, L. (2023). Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma. Oncology Letters, 26, 395. https://doi.org/10.3892/ol.2023.13981
MLA
Lu, J., Zhang, Q., Mo, L., Chen, W., Mao, L."Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma". Oncology Letters 26.3 (2023): 395.
Chicago
Lu, J., Zhang, Q., Mo, L., Chen, W., Mao, L."Comprehensive analysis of E47‑like factors and verification of ELF4 in clear cell renal cell carcinoma". Oncology Letters 26, no. 3 (2023): 395. https://doi.org/10.3892/ol.2023.13981
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