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Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC

  • Authors:
    • Xiaoyan Liu
    • Lili Zeng
    • Wenlong Wang
    • Zhipeng Li
    • Siyuan Zhou
    • Fang Wang
    • Yue Wang
    • Jing Du
    • Xiangrui Ma
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Binzhou Medical University Hospital, Binzhou, Shandong 256600, P.R. China, Department of Stomatology, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing, Zhejiang 314001, P.R. China, Department of Stomatology, Zibo Central Hospital, Zibo, Shandong 255000, P.R. China, Medical Research Center, Binzhou Medical University Hospital, Binzhou, Shandong 256600, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 435
    |
    Published online on: August 22, 2023
       https://doi.org/10.3892/ol.2023.14022
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Abstract

Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. The present study aimed to screen and explore the key circRNA/microRNA (miRNA or miR)/mRNA interactions in head and neck squamous cell carcinoma (HNSCC) using bioinformatics. A total of six pairs of cancerous and adjacent healthy tissue were obtained from patients with HNSCC and genome‑wide transcriptional sequencing was performed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). Moreover, expression levels of DEGs were verified in HNSCC cells and tissues using reverse transcription‑quantitative (RT‑q)PCR. A molecular regulatory network consisting of three circRNAs, seven miRNAs and seven mRNAs was constructed, resulting in identification of two signaling axes, hsa_circ_0035431/hsa‑miR‑940/fucosyltransferase 6 (FUT6) and hsa_circ_0035431/hsa‑miR‑940/cingulin‑like 1 (CGNL1). FUT6 and CGNL1 were downregulated in HNSCC compared with adjacent healthy tissue and the expression levels of these genes were associated with tumor stage. Low FUT6 and CGNL1 expression levels were associated with lower overall survival rate and progression‑free intervals in HNSCC. RT‑qPCR demonstrated that hsa_circ_0035431, FUT6 and CGNL1 were downregulated in HNSCC cells and tissue and hsa‑miR‑940 was upregulated. Notably, these results were consistent with those obtained using high‑throughput sequencing. In conclusion, hsa_circ_0035431 may participate in regulation of FUT6 and CGNL1 expression by sponging hsa‑miR‑940, thus, impacting the occurrence, development and prognosis of HNSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Zeng L, Wang W, Li Z, Zhou S, Wang F, Wang Y, Du J and Ma X: Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC. Oncol Lett 26: 435, 2023.
APA
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F. ... Ma, X. (2023). Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC. Oncology Letters, 26, 435. https://doi.org/10.3892/ol.2023.14022
MLA
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F., Wang, Y., Du, J., Ma, X."Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC". Oncology Letters 26.4 (2023): 435.
Chicago
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F., Wang, Y., Du, J., Ma, X."Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC". Oncology Letters 26, no. 4 (2023): 435. https://doi.org/10.3892/ol.2023.14022
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Zeng L, Wang W, Li Z, Zhou S, Wang F, Wang Y, Du J and Ma X: Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC. Oncol Lett 26: 435, 2023.
APA
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F. ... Ma, X. (2023). Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC. Oncology Letters, 26, 435. https://doi.org/10.3892/ol.2023.14022
MLA
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F., Wang, Y., Du, J., Ma, X."Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC". Oncology Letters 26.4 (2023): 435.
Chicago
Liu, X., Zeng, L., Wang, W., Li, Z., Zhou, S., Wang, F., Wang, Y., Du, J., Ma, X."Integrated analysis of high‑throughput sequencing reveals the regulatory potential of hsa_circ_0035431 in HNSCC". Oncology Letters 26, no. 4 (2023): 435. https://doi.org/10.3892/ol.2023.14022
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