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Article Open Access

FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma

  • Authors:
    • Peipei Li
    • Geng Ma
    • Zhaobo Cui
    • Shusen Zhang
    • Qiao Su
    • Zhigang Cai
  • View Affiliations / Copyright

    Affiliations: The First Department of Pulmonary and Critical Care Medicine, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Gastroenterology, Hengshui People's Hospital, Hengshui, Hebei 053000, P.R. China, Department of Pulmonary and Critical Care Medicine, Hengshui People's Hospital, Hengshui, Hebei 053000, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 518
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    Published online on: October 17, 2023
       https://doi.org/10.3892/ol.2023.14105
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Abstract

Lung adenocarcinoma (LUAD) is a clinically challenging disease due to its poor prognosis and limited therapeutic methods. The aim of the present study was to identify prognosis‑related genes and therapeutic targets for LUAD. Raw data from the GSE32863, GSE41271 and GSE42127 datasets were downloaded from the Gene Expression Omnibus database. Following normalization, the data were merged into a matrix, which was first used to identify differentially expressed genes (DEGs). Weighted gene co‑expression network analysis (WGCNA) and survival analysis were performed to screen potential prognosis‑related genes. Gene overlaps among DEGs, survival‑related genes and WGCNA genes were finally constructed to obtain candidate genes. An analysis with the STRING database was performed to construct a protein‑protein interaction network and hub genes were selected using Cytoscape. The candidate genes were finally identified by univariate and multivariate Cox regression analysis. Furthermore, in vivo and in vitro experiments, including immunohistochemistry, immunofluorescence, Cell Counting Kit‑8, colony‑formation and migration assays, were performed to validate the potential mechanism of these genes in LUAD. Two genes, namely forkhead box M1 (FOXM1) and centromere protein F (CENPF), were identified as unfavorable indicators of prognosis in patients with LUAD. High expression of FOXM1 and CENPF were associated with poor survival. Furthermore, LUAD cells with FOXM1 and CENPF knockdown showed a significant reduction in proliferation and migration (P<0.05). FOXM1 and CENPF may have an essential role in the prognosis of patients with LUAD by influencing cell proliferation and migration, and they provide potential molecular targets for LUAD therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Li P, Ma G, Cui Z, Zhang S, Su Q and Cai Z: FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma. Oncol Lett 26: 518, 2023.
APA
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., & Cai, Z. (2023). FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma. Oncology Letters, 26, 518. https://doi.org/10.3892/ol.2023.14105
MLA
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., Cai, Z."FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma". Oncology Letters 26.6 (2023): 518.
Chicago
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., Cai, Z."FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma". Oncology Letters 26, no. 6 (2023): 518. https://doi.org/10.3892/ol.2023.14105
Copy and paste a formatted citation
x
Spandidos Publications style
Li P, Ma G, Cui Z, Zhang S, Su Q and Cai Z: FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma. Oncol Lett 26: 518, 2023.
APA
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., & Cai, Z. (2023). FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma. Oncology Letters, 26, 518. https://doi.org/10.3892/ol.2023.14105
MLA
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., Cai, Z."FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma". Oncology Letters 26.6 (2023): 518.
Chicago
Li, P., Ma, G., Cui, Z., Zhang, S., Su, Q., Cai, Z."FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma". Oncology Letters 26, no. 6 (2023): 518. https://doi.org/10.3892/ol.2023.14105
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