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Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2

  • Authors:
    • Xiangtian Shi
    • Jiarui Yang
    • Meng Wang
    • Long Xia
    • Lei Zhang
    • Shan Qiao
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    Affiliations: Department of Hepatobiliary Surgery, Bayannur Hospital, Bayannur, Inner Mongolia Autonomous Region 015000, P.R. China, Department of Hepatobiliary, Pancreatic and Splenic Surgery, Affiliated Dongguan Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong 523058, P.R. China, Department of Hepatobiliary‑Pancreatic‑Splenic Surgery, Inner Mongolia Autonomous Region People's Hospital, Hohhot, Inner Mongolia Autonomous Region 010017, P.R. China, Department of Pancreatic Hepato‑Biliary‑Surgery, The Sixth Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China
    Copyright: © Shi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 2
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    Published online on: November 6, 2023
       https://doi.org/10.3892/ol.2023.14135
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Abstract

Intrahepatic cholangiocarcinoma (ICC) is a highly lethal hepatobiliary tumor with high aggressiveness. The role of circular RNA (circRNA) in ICC remains to be explored. The present study aimed to investigate whether hsa_circ_0050900 affected ferroptosis in ICC cells by regulating hsa‑microRNA (miR)‑605‑3p/solute carrier family 3 member 2 (SLC3A2). Human ICC cells were cultured and hsa_circ_0050900 expression was evaluated by reverse transcription‑quantitative PCR. hsa_circ_0050900 was knocked down and ferroptosis inhibitor ferrostatin‑1 was added to HuCCT‑1 cells. Following knockdown or overexpression of hsa‑miR‑605‑3p, Fe2+, reactive oxygen species (ROS), glutathione peroxidase 4 and SLC3A2 levels were assessed using iron and ROS assay kit or RT‑qPCR and western blotting, respectively. Cell function experiments were performed to examine proliferation and migration abilities. Dual‑luciferase reporter gene and argonaute2‑RNA immunoprecipitation assay verified the relationship among hsa_circ_0050900, hsa‑miR‑605‑3p, and SLC3A2. hsa_circ_0050900 was derived from actinin alpha 4 gene and was elevated in ICC cells. Among HuCCT‑1, QBC‑939, HCCC‑9810, and RBE cell lines, the highest expression was in HuCCT‑1 cells. Inhibition of hsa_circ_0050900 inhibited proliferation and migration by facilitating ICC cell ferroptosis. hsa‑miR‑605‑3p expression was elevated after knocking down hsa_circ_0050900 and hsa‑miR‑605‑3p was negatively regulated by hsa_circ_0050900. In addition, hsa‑miR‑605‑3p targeted SLC3A2. Overexpression of hsa‑miR‑605‑3p regulated SLC3A2 to promote ICC cell ferroptosis and inhibit proliferation and migration. Taken together, knockdown of hsa_circ_0050900 inhibited SLC3A2 expression via sponging hsa‑miR‑605‑3p to promote ICC cell ferroptosis, and finally suppressed proliferation and migration. The present study suggested that hsa_circ_0050900 was a potential therapeutic target for ICC.
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Copy and paste a formatted citation
Spandidos Publications style
Shi X, Yang J, Wang M, Xia L, Zhang L and Qiao S: Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2. Oncol Lett 27: 2, 2024.
APA
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., & Qiao, S. (2024). Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2. Oncology Letters, 27, 2. https://doi.org/10.3892/ol.2023.14135
MLA
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., Qiao, S."Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2". Oncology Letters 27.1 (2024): 2.
Chicago
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., Qiao, S."Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2". Oncology Letters 27, no. 1 (2024): 2. https://doi.org/10.3892/ol.2023.14135
Copy and paste a formatted citation
x
Spandidos Publications style
Shi X, Yang J, Wang M, Xia L, Zhang L and Qiao S: Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2. Oncol Lett 27: 2, 2024.
APA
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., & Qiao, S. (2024). Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2. Oncology Letters, 27, 2. https://doi.org/10.3892/ol.2023.14135
MLA
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., Qiao, S."Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2". Oncology Letters 27.1 (2024): 2.
Chicago
Shi, X., Yang, J., Wang, M., Xia, L., Zhang, L., Qiao, S."Hsa_circ_0050900 affects ferroptosis in intrahepatic cholangiocarcinoma cells by targeting hsa‑miR-605‑3p to regulate SLC3A2". Oncology Letters 27, no. 1 (2024): 2. https://doi.org/10.3892/ol.2023.14135
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