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Article Special Issue

mRNA expression of CRF family members in urothelial bladder cancer

This article is part of the special Issue: Clinical and Molecular Insights on Urothelial Carcinoma
  • Authors:
    • Charalampos Mavridis
    • Maria Venihaki
    • Eirini Dermitzaki
    • Michail Deiktakis
    • Georgios Liapakis
    • Charalampos Mamoulakis
  • View Affiliations / Copyright

    Affiliations: Department of Urology, University General Hospital of Heraklion, Medical School, University of Crete, Heraklion 71003, Greece, Department of Clinical Chemistry, Medical School, University of Crete, Heraklion 71003, Greece, Department of Pharmacology, Medical School, University of Crete, Heraklion 71003, Greece
  • Article Number: 13
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    Published online on: November 10, 2023
       https://doi.org/10.3892/ol.2023.14145
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Abstract

The corticotropin‑releasing factor (CRF) gene family includes the three urocortins (UCN1, 2 and 3) and the two receptors (CRFR1 and 2), which play a significant role in the physiology of various organs. The expression of the CRF family of genes and its receptors are shown to participate in the pathogenesis of inflammation and even tumorigenesis. However, data regarding the human urinary tract, especially the bladder, are scarce. To the best of our knowledge, no studies are currently available on the CRF system and bladder cancer. The primary goal of the present study was to investigate the mRNA expression of the CRF family members in bladder cancer. The secondary aim was to analyze the differences with the expression of the same mRNAs in normal bladders. From August 2018 to July 2021, 43 recruited patients were divided into three groups. Group A included healthy patients, group B included patients with bladder cancer and group C included patients with a history of cancer from whom samples were taken from the normal bladder mucosa. Detection of mRNA of the CRF family of genes was performed using reverse transcription‑quantitative PCR. The mRNA of the three urocortins, CRF and the two receptors were predominantly expressed in all three groups of patients. Statistical analysis using the Kruskal‑Wallis test showed that UCN1 was downregulated in patients with bladder cancer and those with possible cancer compared with the healthy group (mean rank group A=24.3 vs. mean rank group B=12.58; P=0.006) and (mean rank group A=24.3 vs. mean rank group C=8.88; P=0.001). The present experiments showed that mRNA of the CRF family of genes was amplified in normal and cancer bladder tissues. Downregulation of the UCN1 gene may be associated with bladder cancer, contributing to the prognosis, diagnosis or therapy of urothelial malignancies.
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Copy and paste a formatted citation
Spandidos Publications style
Mavridis C, Venihaki M, Dermitzaki E, Deiktakis M, Liapakis G and Mamoulakis C: mRNA expression of CRF family members in urothelial bladder cancer. Oncol Lett 27: 13, 2024.
APA
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., & Mamoulakis, C. (2024). mRNA expression of CRF family members in urothelial bladder cancer. Oncology Letters, 27, 13. https://doi.org/10.3892/ol.2023.14145
MLA
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., Mamoulakis, C."mRNA expression of CRF family members in urothelial bladder cancer". Oncology Letters 27.1 (2024): 13.
Chicago
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., Mamoulakis, C."mRNA expression of CRF family members in urothelial bladder cancer". Oncology Letters 27, no. 1 (2024): 13. https://doi.org/10.3892/ol.2023.14145
Copy and paste a formatted citation
x
Spandidos Publications style
Mavridis C, Venihaki M, Dermitzaki E, Deiktakis M, Liapakis G and Mamoulakis C: mRNA expression of CRF family members in urothelial bladder cancer. Oncol Lett 27: 13, 2024.
APA
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., & Mamoulakis, C. (2024). mRNA expression of CRF family members in urothelial bladder cancer. Oncology Letters, 27, 13. https://doi.org/10.3892/ol.2023.14145
MLA
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., Mamoulakis, C."mRNA expression of CRF family members in urothelial bladder cancer". Oncology Letters 27.1 (2024): 13.
Chicago
Mavridis, C., Venihaki, M., Dermitzaki, E., Deiktakis, M., Liapakis, G., Mamoulakis, C."mRNA expression of CRF family members in urothelial bladder cancer". Oncology Letters 27, no. 1 (2024): 13. https://doi.org/10.3892/ol.2023.14145
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