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CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway

  • Authors:
    • Ping Tian
    • Jinglan Deng
    • Cailing Ma
    • Ainipa Miershali
    • Gulikezi Maimaitirexiati
    • Qi Yan
    • Yating Liu
    • Hatimihan Maimaiti
    • Yuting Li
    • Changhui Zhou
    • Jingqin Ren
    • Lu Ding
    • Rong Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China, College of Nursing, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China, Department of Child, Adolescent and Maternal Hygiene, College of Public Health, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China, Department of Child, Adolescent and Maternal Hygiene, College of Public Health, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China, Postdoctoral Research Center on Public Health and Preventive Medicine, Xinjiang Medical University, Urumqi, Xinjiang Uyghur Autonomous Region 830054, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 14
    |
    Published online on: November 13, 2023
       https://doi.org/10.3892/ol.2023.14147
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Abstract

The chromobox protein homolog 7 (CBX7) serves a tumor‑suppressive role in human malignant neoplasias. The downregulation of CBX7 is associated with the poor prognosis and aggressiveness of various human cancers. However, the biological functions and underlying mechanisms of CBX7 in cervical cancer remain unclear. The present study investigated the role and mechanism of CBX7 in cervical cancer. Lentivirus and siRNA were used to construct cervical cancer cells with stable CBX7 knockdown and SiHa xenograft models. The cell growth, migration, invasion and apoptosis were observed through in vivo and in vitro experiments. The expression levels of CBX7, integrin β3 (ITGβ3), transforming growth factor β1 (TGFβ1), phosphatidylinositol‑3‑kinase (PI3K), AKT, E‑cadherin (E‑cad) and vimentin (VIM) were detected by western blot analysis and reverse transcription‑quantitative PCR. The correlation between CBX7 and these genes was analyzed. TGFβ1 was also silenced through shRNA in cells with stable CBX7 knockdown to detect its effect on cell growth, invasion and apoptosis, and on pathway‑related gene expression. It was revealed that knockdown of CBX7 promoted the proliferation, migration, and invasion of cervical cancer cells, and inhibited apoptosis. In addition, CBX7 knockdown promoted tumor growth in vivo. Correlation analysis demonstrated that CBX7 was negatively correlated with ITGβ3, TGFβ1, PI3K, AKT, phosphorylated AKT and VIM, but positively correlated with E‑cad. Moreover, the knockdown of TGFβ1 reversed the promotion of cell proliferation and inhibition of apoptosis induced by CBX7 knockdown and attenuated the increase of ITGβ3, TGFβ1, PI3K, AKT and VIM caused by CBX7 knockdown. In conclusion, the findings of the present study indicated that the downregulation of CBX7 enhances cell migration and invasion while inhibiting cell apoptosis in cervical cancer by modulating the ITGβ3/TGFβ1/AKT signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Tian P, Deng J, Ma C, Miershali A, Maimaitirexiati G, Yan Q, Liu Y, Maimaiti H, Li Y, Zhou C, Zhou C, et al: CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway. Oncol Lett 27: 14, 2024.
APA
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q. ... Li, R. (2024). CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway. Oncology Letters, 27, 14. https://doi.org/10.3892/ol.2023.14147
MLA
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q., Liu, Y., Maimaiti, H., Li, Y., Zhou, C., Ren, J., Ding, L., Li, R."CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway". Oncology Letters 27.1 (2024): 14.
Chicago
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q., Liu, Y., Maimaiti, H., Li, Y., Zhou, C., Ren, J., Ding, L., Li, R."CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway". Oncology Letters 27, no. 1 (2024): 14. https://doi.org/10.3892/ol.2023.14147
Copy and paste a formatted citation
x
Spandidos Publications style
Tian P, Deng J, Ma C, Miershali A, Maimaitirexiati G, Yan Q, Liu Y, Maimaiti H, Li Y, Zhou C, Zhou C, et al: CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway. Oncol Lett 27: 14, 2024.
APA
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q. ... Li, R. (2024). CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway. Oncology Letters, 27, 14. https://doi.org/10.3892/ol.2023.14147
MLA
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q., Liu, Y., Maimaiti, H., Li, Y., Zhou, C., Ren, J., Ding, L., Li, R."CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway". Oncology Letters 27.1 (2024): 14.
Chicago
Tian, P., Deng, J., Ma, C., Miershali, A., Maimaitirexiati, G., Yan, Q., Liu, Y., Maimaiti, H., Li, Y., Zhou, C., Ren, J., Ding, L., Li, R."CBX7 is involved in the progression of cervical cancer through the ITGβ3/TGFβ1/AKT pathway". Oncology Letters 27, no. 1 (2024): 14. https://doi.org/10.3892/ol.2023.14147
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