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Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway

  • Authors:
    • Xin Wei
    • Jinliang Ni
    • Lin Yuan
    • Xueliang Li
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, First Clinical Medical College, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 17
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    Published online on: November 15, 2023
       https://doi.org/10.3892/ol.2023.14150
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Abstract

Esophageal cancer is one of the most common cancer types in humans worldwide. Photodynamic therapy (PDT) is a promising therapeutic strategy for the treatment of cancer. However, its underlying mechanism needs to be studied thoroughly. The present study focused on the antitumor effect and underlying mechanism of the use of hematoporphyrin derivative (HpD)‑PDT against human esophageal squamous cell carcinoma cells via regulation of the PI3K/AKT/mTOR signaling pathway. A Cell Counting Kit‑8 assay was used to measure cell viability. Migration was evaluated using a wound healing assay. An annexin V‑FITC/PI kit was used to determine cell apoptosis rates. Protein expression levels were analyzed via western blotting. Reverse transcription‑quantitative PCR was used to detect gene expression levels. A 2',7'‑dichlorodihydrofluorescein diacetate kit was chosen to evaluate intracellular reactive oxygen species levels via flow cytometry. Cell viability and migration were decreased in KYSE‑150 cells after HpD‑PDT treatment. Cellular apoptosis was induced after HpD‑PDT treatment, and the same trend was observed for autophagy. Furthermore, the PI3K/AKT/mTOR signaling pathway was inhibited. The viability and migration of KYSE‑150 cells were significantly inhibited, and apoptosis was induced more effectively following treatment with a combination of HpD‑PDT and the PI3K inhibitor, a final concentration of 20 µM LY294002. In conclusion, HpD‑PDT could suppress esophageal cancer cell viability, induce apoptosis and inhibit migration by downregulating the PI3K/AKT/mTOR signaling pathway. Combination of HpD‑PDT with PI3K inhibitor (LY294002) could enhance the therapeutic efficacy compared with that demonstrated by HpD‑PDT alone. Further studies on combination therapy are required to achieve improved clinical outcomes.
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Copy and paste a formatted citation
Spandidos Publications style
Wei X, Ni J, Yuan L and Li X: Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway. Oncol Lett 27: 17, 2024.
APA
Wei, X., Ni, J., Yuan, L., & Li, X. (2024). Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway. Oncology Letters, 27, 17. https://doi.org/10.3892/ol.2023.14150
MLA
Wei, X., Ni, J., Yuan, L., Li, X."Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway". Oncology Letters 27.1 (2024): 17.
Chicago
Wei, X., Ni, J., Yuan, L., Li, X."Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway". Oncology Letters 27, no. 1 (2024): 17. https://doi.org/10.3892/ol.2023.14150
Copy and paste a formatted citation
x
Spandidos Publications style
Wei X, Ni J, Yuan L and Li X: Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway. Oncol Lett 27: 17, 2024.
APA
Wei, X., Ni, J., Yuan, L., & Li, X. (2024). Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway. Oncology Letters, 27, 17. https://doi.org/10.3892/ol.2023.14150
MLA
Wei, X., Ni, J., Yuan, L., Li, X."Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway". Oncology Letters 27.1 (2024): 17.
Chicago
Wei, X., Ni, J., Yuan, L., Li, X."Hematoporphyrin derivative photodynamic therapy induces apoptosis and suppresses the migration of human esophageal squamous cell carcinoma cells by regulating the PI3K/AKT/mTOR signaling pathway". Oncology Letters 27, no. 1 (2024): 17. https://doi.org/10.3892/ol.2023.14150
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