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Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation

  • Authors:
    • Yufei Li
    • Jiayi Shi
    • Zhixin Liu
    • Yonggang Lin
    • An Xie
    • Wenxiu Sun
    • Jiaqi Liu
    • Jun Liang
  • View Affiliations / Copyright

    Affiliations: Morphology Laboratory, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, School of Life Sciences, Mudanjiang Medical University, Mudanjiang, Heilongjiang 157011, P.R. China, Department of Extracorporeal Circulation, Mudanjiang Cardiovascular Disease Hospital, Mudanjiang, Heilongjiang 157011, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 60
    |
    Published online on: December 18, 2023
       https://doi.org/10.3892/ol.2023.14194
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Abstract

Cell surface markers are most widely used in the study of cancer stem cells (CSCs). However, cell surface markers that are safely and stably expressed in CSCs have yet to be identified. Colonic CSCs express leukocyte CD14. CD14 binding to the ligand lipopolysaccharide (LPS) is involved in the inflammatory response via the Toll‑like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88) signaling pathway. TLR4 and MyD88 have been reported to promote the proliferation, metastasis and tumorigenicity of colon cancer cells, which is consistent with the characteristics of CSCs. In the present study, the proposed experimental method to detect cell proliferation, metastasis and tumorigenesis was used to confirm that, under LPS stimulation, CD14 promoted the proliferation, migration and tumorigenesis of colonic CSCs via the TLR4/MyD88 signaling pathway. Cell Counting Kit‑8 and 5‑ethynyl‑2'‑deoxyuridine assays were used to assess the proliferation and migration of the cells. Colony formation and nude mouse xenograft assays were used to assess the capacity of cells to form tumors. Using western blotting and reverse transcription‑quantitative PCR, the mRNA and protein levels of CD14, TLR4 and MyD88 were examined. It was confirmed that CD14 promoted the proliferation, metastasis and tumorigenesis of colon CSCs in response to LPS stimulation via the TLR4/MyD88 signaling pathway, and CD14+ colon cancer cells were successfully isolated and sorted. According to the results of proliferation assay, it was determined that CD14 regulated the LPS‑induced proliferation of colon CSCs. CD14, TLR4 and MyD88 protein and mRNA expression was upregulated in colon CSCs in response to LPS stimulation. This indicates a potential novel target for colon CSC‑related studies.
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Copy and paste a formatted citation
Spandidos Publications style
Li Y, Shi J, Liu Z, Lin Y, Xie A, Sun W, Liu J and Liang J: Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation. Oncol Lett 27: 60, 2024.
APA
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W. ... Liang, J. (2024). Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation. Oncology Letters, 27, 60. https://doi.org/10.3892/ol.2023.14194
MLA
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W., Liu, J., Liang, J."Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation". Oncology Letters 27.2 (2024): 60.
Chicago
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W., Liu, J., Liang, J."Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation". Oncology Letters 27, no. 2 (2024): 60. https://doi.org/10.3892/ol.2023.14194
Copy and paste a formatted citation
x
Spandidos Publications style
Li Y, Shi J, Liu Z, Lin Y, Xie A, Sun W, Liu J and Liang J: Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation. Oncol Lett 27: 60, 2024.
APA
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W. ... Liang, J. (2024). Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation. Oncology Letters, 27, 60. https://doi.org/10.3892/ol.2023.14194
MLA
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W., Liu, J., Liang, J."Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation". Oncology Letters 27.2 (2024): 60.
Chicago
Li, Y., Shi, J., Liu, Z., Lin, Y., Xie, A., Sun, W., Liu, J., Liang, J."Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation". Oncology Letters 27, no. 2 (2024): 60. https://doi.org/10.3892/ol.2023.14194
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