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A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma

  • Authors:
    • Federico Maria Gioacchini
    • Arianna Di Stadio
    • Pietro De Luca
    • Angelo Camaioni
    • Annalisa Pace
    • Giannicola Iannella
    • Corrado Rubini
    • Marco Santarelli
    • Marco Tomasetti
    • Alfonso Scarpa
    • Fabiola Olivieri
    • Massimo Re
  • View Affiliations / Copyright

    Affiliations: Ear, Nose and Throat Unit, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona Joint Hospitals, I‑60020 Ancona, Italy, Gian Filippo Ingrassia Department, Otolaryngology Unit, University of Catania, I‑95121 Catania, Italy, Department of Otolaryngology, Fatebenefratelli Isola Tiberina‑Gemelli Hospital, I‑00100 Rome, Italy, Head and Neck Department, San Giovanni‑Addolorata Hospital, I‑00189 Rome, Italy, Department of Sense Organs, University La Sapienza of Rome, I‑00161 Rome, Italy, Pathology and Histopathology Division, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, I‑60020 Ancona, Italy, Department of Clinical and Molecular Sciences, Section of Occupational Medicine, Polytechnic University of Marche, I‑60020 Ancona, Italy, Department of Medicine and Surgery, University of Salerno, I‑84084 Fisciano, Italy, Clinic of Laboratory and Precision Medicine, IRCCS INRCA, I-60121 Ancona, Italy
    Copyright: © Gioacchini et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 69
    |
    Published online on: December 20, 2023
       https://doi.org/10.3892/ol.2023.14202
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Abstract

Despite its histological resemblance to colorectal adenocarcinoma, there is little information about the molecular events involved in the pathogenesis of intestinal‑type sinonasal adenocarcinoma (ITAC). The present study investigated the possible role and clinical value of microRNA (miR)‑let‑7a, a head and neck squamous cell carcinoma‑related miR, in a well‑characterized and homogeneous cohort of patients with ethmoidal ITAC associated with occupational exposure, treated by primary surgery. miR‑let‑7a expression levels were analyzed in 23 pairs of ethmoidal ITAC and adjacent normal formalin‑fixed paraffin‑embedded tissues by reverse transcription‑quantitative PCR. The expression was evaluated in tumor and healthy tissues according to: Tumor grade (G) of differentiation and extension, and pTNM stage, and presence/absence of recurrence. Comparisons within and between groups were performed using two‑tailed Student's paired t‑test and one‑way ANOVA with Tukey's post hoc test. P<0.05 was considered to indicate a statistically significant difference. miR‑let‑7a expression in ethmoidal ITAC tissues was significantly lower than that in adjacent normal tissues (P<0.05; mean expression level ± SD, 1.452707±1.4367189 vs. 4.094017±2.7465375). miR expression varied with pT stage. miR‑let‑7a was downregulated (P<0.05) in advanced stages (pT3‑pT4) compared with earlier stages (pT1‑pT2). Furthermore, downregulation of miR‑let‑7a in ITAC was associated with poorly‑differentiated (G3) cancer (P<0.05). No other associations were observed between miR‑let‑7a expression and the other clinicopathological parameters, including disease‑free survival. In conclusion, downregulation of miR‑let‑7a in ITAC was associated with advanced‑stage (pT3 and pT4) and poorly‑differentiated (G3) disease, suggesting that the mutation of this gene, combined with additional genetic events, could serve a role in ITAC pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Gioacchini FM, Di Stadio A, De Luca P, Camaioni A, Pace A, Iannella G, Rubini C, Santarelli M, Tomasetti M, Scarpa A, Scarpa A, et al: A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma. Oncol Lett 27: 69, 2024.
APA
Gioacchini, F.M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G. ... Re, M. (2024). A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma. Oncology Letters, 27, 69. https://doi.org/10.3892/ol.2023.14202
MLA
Gioacchini, F. M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G., Rubini, C., Santarelli, M., Tomasetti, M., Scarpa, A., Olivieri, F., Re, M."A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma". Oncology Letters 27.2 (2024): 69.
Chicago
Gioacchini, F. M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G., Rubini, C., Santarelli, M., Tomasetti, M., Scarpa, A., Olivieri, F., Re, M."A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma". Oncology Letters 27, no. 2 (2024): 69. https://doi.org/10.3892/ol.2023.14202
Copy and paste a formatted citation
x
Spandidos Publications style
Gioacchini FM, Di Stadio A, De Luca P, Camaioni A, Pace A, Iannella G, Rubini C, Santarelli M, Tomasetti M, Scarpa A, Scarpa A, et al: A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma. Oncol Lett 27: 69, 2024.
APA
Gioacchini, F.M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G. ... Re, M. (2024). A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma. Oncology Letters, 27, 69. https://doi.org/10.3892/ol.2023.14202
MLA
Gioacchini, F. M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G., Rubini, C., Santarelli, M., Tomasetti, M., Scarpa, A., Olivieri, F., Re, M."A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma". Oncology Letters 27.2 (2024): 69.
Chicago
Gioacchini, F. M., Di Stadio, A., De Luca, P., Camaioni, A., Pace, A., Iannella, G., Rubini, C., Santarelli, M., Tomasetti, M., Scarpa, A., Olivieri, F., Re, M."A pilot study to evaluate the expression of microRNA‑let‑7a in patients with intestinal‑type sinonasal adenocarcinoma". Oncology Letters 27, no. 2 (2024): 69. https://doi.org/10.3892/ol.2023.14202
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