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Article Special Issue Open Access

Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity

This article is part of the special Issue: Molecular target and action mechanism of anti-cancer agents
  • Authors:
    • Sang Eun Ha
    • Anjugam Paramanantham
    • Hun Hwan Kim
    • Pritam Bhagwan Bhosale
    • Min Yeong Park
    • Abuyaseer Abusaliya
    • Jeong Doo Heo
    • Won Sup Lee
    • Gon Sup Kim
  • View Affiliations / Copyright

    Affiliations: Research Institute of Life Science and College of Veterinary Medicine, Gyeongsang National University, Jinju, Gyeongsangnam‑do 52828, Republic of Korea, Gyeongnam Bio‑Health Research Support Center, Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jinju, Gyeongsangnam‑do 52834, Republic of Korea, Department of Internal Medicine, Institute of Health Sciences and Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, Jinju, Gyeongsangnam‑do 52727, Republic of Korea
    Copyright: © Ha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 94
    |
    Published online on: January 11, 2024
       https://doi.org/10.3892/ol.2024.14227
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Abstract

Worldwide, liver cancer is the most frequent fatal malignancy. Liver cancer prognosis is poor because patients frequently receive advanced‑stage diagnoses. The current study aimed to establish the potential pharmacological targets and the biological networks of scutellarein (SCU) in liver cancer, a natural product known to have low toxicity and side effects. To identify the differentially expressed genes between SCU‑treated and SCU‑untreated HepG2 cells, RNA sequencing (RNA‑seq) was carried out. A total of 463 genes were revealed to have differential expression, of which 288 were upregulated and 175 were downregulated in the group that had received SCU treatment compared with a control group. Gene Ontology (GO) enrichment analysis of associated biological process terms revealed they were mostly involved in the regulation of protein heterodimerization activity and nucleosomes. Interaction of protein‑protein network analysis using Search Tool for the Retrieval of Interacting Genes/Proteins resulted in two crucial interacting hub targets; namely, histone H1‑4 and protein tyrosine phosphatase receptor type C. Additionally, the crucial targets were validated using western blotting. Overall, the present study demonstrated that the use of RNA‑seq data, with bioinformatics tools, can provide a valuable resource to identify the pharmacological targets that could have important biological roles in liver cancer.
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Ha S, Paramanantham A, Kim H, Bhosale P, Park M, Abusaliya A, Heo J, Lee W and Kim G: Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity. Oncol Lett 27: 94, 2024.
APA
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A. ... Kim, G. (2024). Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity. Oncology Letters, 27, 94. https://doi.org/10.3892/ol.2024.14227
MLA
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A., Heo, J., Lee, W., Kim, G."Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity". Oncology Letters 27.3 (2024): 94.
Chicago
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A., Heo, J., Lee, W., Kim, G."Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity". Oncology Letters 27, no. 3 (2024): 94. https://doi.org/10.3892/ol.2024.14227
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Spandidos Publications style
Ha S, Paramanantham A, Kim H, Bhosale P, Park M, Abusaliya A, Heo J, Lee W and Kim G: Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity. Oncol Lett 27: 94, 2024.
APA
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A. ... Kim, G. (2024). Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity. Oncology Letters, 27, 94. https://doi.org/10.3892/ol.2024.14227
MLA
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A., Heo, J., Lee, W., Kim, G."Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity". Oncology Letters 27.3 (2024): 94.
Chicago
Ha, S., Paramanantham, A., Kim, H., Bhosale, P., Park, M., Abusaliya, A., Heo, J., Lee, W., Kim, G."Comprehensive transcriptomic profiling of liver cancer identifies that histone and PTEN are major regulators of SCU‑induced antitumor activity". Oncology Letters 27, no. 3 (2024): 94. https://doi.org/10.3892/ol.2024.14227
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