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Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report

  • Authors:
    • Wataru Ando
    • Masaya Sogabe
    • Shigemi Ishikawa
    • Takayuki Uematsu
    • Hiroyuki Furuya
    • Hiroaki Yokomori
    • Yutaka Kohgo
    • Katsuya Otori
    • Tomoyuki Nakano
    • Shunsuke Endo
    • Hiroyoshi Tsubochi
    • Isao Okazaki
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Pharmacy, Center for Clinical Pharmacy and Sciences, Kitasato University School of Pharmacy, Tokyo 108‑8641, Japan, Department of General Thoracic Surgery, Jichi Medical University Saitama Medical Center, Saitama 330‑0834, Japan, Center for Respiratory Diseases, International University of Health and Welfare Hospital, Nasu‑Shiobara, Tochigi 329‑2763, Japan, Biomedical Laboratory, Division of Biomedical Research, Kitasato University Medical Center, Kitamoto, Saitama 364‑8501, Japan, Basic Clinical Science and Public Health, Tokai University School of Medicine, Isehara, Kanagawa 259‑1193, Japan, Department of Internal Medicine, Kitasato University Medical Center, Kitamoto, Saitama 364‑8501, Japan, Department of Internal Medicine, International University of Health and Welfare Hospital, Nasu‑Shiobara, Tochigi 329‑2763, Japan, Research and Education Center for Clinical Pharmacy, Division of Clinical Pharmacy, Laboratory of Pharmacy Practice and Science 1, Kitasato University School of Pharmacy, Sagamihara, Kanagawa 252‑0375, Japan, Department of General Thoracic Surgery, Jichi Medical University, Shimotsuke, Tochigi 329‑0498, Japan, Department of Health and Welfare, Higashi Nippon International University, Iwaki, Fukushima 970‑8023, Japan
    Copyright: © Ando et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 127
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    Published online on: January 29, 2024
       https://doi.org/10.3892/ol.2024.14261
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Abstract

The present study describes a novel molecular‑genetic method suitable for lung cancer (LC) screening in the work‑place and at community health centers. Using urinary‑isolated exosomes from 35 patients with LC and 40 healthy volunteers, the expression ratio of MMP‑1/CD63, and the relative expression levels of both microRNA (miRNA)‑21 and miRNA‑486‑5p were measured. MMP‑1/CD63 expression ratio was significantly higher in patients with LC than in the healthy controls {1.342 [95% confidence interval (CI): 0.890‑1.974] vs. 0.600 (0.490‑0.900); P<0.0001}. The relative expression of miRNA‑486‑5p in male healthy controls was significantly different from that in female healthy controls, whereas there was no significant difference in miRNA‑21. Receiver operating characteristic curve (ROC) analysis of MMP‑1/CD63 showed 92.5% sensitivity and 54.3% specificity, whereas miRNA‑486‑5p showed 85% sensitivity and 70.8% specificity for men, and 70.0% sensitivity and 72.7% specificity for women. The logistic regression model used to evaluate the association of LC with the combination of MMP‑1/CD63 and miRNA‑486‑5p revealed that the area under the ROC curve was 0.954 (95% CI: 0.908‑1.000), and the model had 89% sensitivity and 88% specificity after adjusting for age, sex and smoking status. These data suggested that the combined analysis of MMP‑1/CD63 and miRNA‑486‑5p in urinary exosomes may be used to detect patients with early‑stage LC in the work‑place and at community health centers, although confirmational studies are warranted.
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Spandidos Publications style
Ando W, Sogabe M, Ishikawa S, Uematsu T, Furuya H, Yokomori H, Kohgo Y, Otori K, Nakano T, Endo S, Endo S, et al: Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report. Oncol Lett 27: 127, 2024.
APA
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H. ... Okazaki, I. (2024). Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report. Oncology Letters, 27, 127. https://doi.org/10.3892/ol.2024.14261
MLA
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H., Kohgo, Y., Otori, K., Nakano, T., Endo, S., Tsubochi, H., Okazaki, I."Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report". Oncology Letters 27.3 (2024): 127.
Chicago
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H., Kohgo, Y., Otori, K., Nakano, T., Endo, S., Tsubochi, H., Okazaki, I."Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report". Oncology Letters 27, no. 3 (2024): 127. https://doi.org/10.3892/ol.2024.14261
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Spandidos Publications style
Ando W, Sogabe M, Ishikawa S, Uematsu T, Furuya H, Yokomori H, Kohgo Y, Otori K, Nakano T, Endo S, Endo S, et al: Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report. Oncol Lett 27: 127, 2024.
APA
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H. ... Okazaki, I. (2024). Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report. Oncology Letters, 27, 127. https://doi.org/10.3892/ol.2024.14261
MLA
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H., Kohgo, Y., Otori, K., Nakano, T., Endo, S., Tsubochi, H., Okazaki, I."Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report". Oncology Letters 27.3 (2024): 127.
Chicago
Ando, W., Sogabe, M., Ishikawa, S., Uematsu, T., Furuya, H., Yokomori, H., Kohgo, Y., Otori, K., Nakano, T., Endo, S., Tsubochi, H., Okazaki, I."Matrix metalloproteinase‑1 and microRNA‑486‑5p in urinary exosomes can be used to detect early lung cancer: A preliminary report". Oncology Letters 27, no. 3 (2024): 127. https://doi.org/10.3892/ol.2024.14261
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