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Article

Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid

  • Authors:
    • Yutaka Nakano
    • Gaku Shimane
    • Kohei Nakamura
    • Reika Takamatsu
    • Eriko Aimono
    • Hiroshi Yagi
    • Yuta Abe
    • Yasushi Hasegawa
    • Shutaro Hori
    • Masayuki Tanaka
    • Yohei Masugi
    • Minoru Kitago
    • Hiroshi Nishihara
    • Yuko Kitagawa
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Keio University School of Medicine, Tokyo 160‑8582, Japan, Genomics Unit, Keio University School of Medicine, Tokyo 160‑8582, Japan, Department of Pathology, Keio University School of Medicine, Tokyo 160‑8582, Japan
  • Article Number: 139
    |
    Published online on: February 2, 2024
       https://doi.org/10.3892/ol.2024.14272
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Abstract

The efficacy of next‑generation sequencing (NGS) of tumor‑derived DNA from intraoperative peritoneal washing fluid (IPWF) of patients with pancreatic ductal adenocarcinoma (PDAC) who intend to undergo curative resection remains unclear. The aim of the present study was to evaluate whether genomic mutations in tumor‑derived DNA from IPWF samples of patients with PDAC who intend to undergo curative resection could be detected using NGS. A total of 12 such patients were included in this study. Cytology of IPWF (CY) was assessed and NGS of genomic tumor‑derived DNA from the IPWF was performed to determine whether genomic mutations could be detected in these patient samples. A total of 2 patients (16.7%) had a CY(+) status and 1 patient (8.3%) showed intraoperative macro‑peritoneal dissemination; 11 patients underwent radical surgery. Actionable gene alterations were detected in 8 (80.0%) out of the 10 patients with CY(‑) status based on NGS of IPWF samples, and 3 (37.5%) patients among those with actionable gene mutations identified from IPWF samples underwent peritoneal dissemination after surgery within ~12 months. The most common genomic mutation was in KRAS (9 patients, 75.0%), followed by TP53 (3 patients, 25.0%), SMAD4 (1 patient, 8.3%) and CDKN2A (1 patient, 8.3%). These findings indicated that the genomic mutations identified in tumor‑derived DNA from IPWF samples of patients with PDAC with a CY(‑) status who intend to undergo curative resection are potential biomarkers for predicting the recurrence of early peritoneal dissemination.
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Copy and paste a formatted citation
Spandidos Publications style
Nakano Y, Shimane G, Nakamura K, Takamatsu R, Aimono E, Yagi H, Abe Y, Hasegawa Y, Hori S, Tanaka M, Tanaka M, et al: Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid. Oncol Lett 27: 139, 2024.
APA
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H. ... Kitagawa, Y. (2024). Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid. Oncology Letters, 27, 139. https://doi.org/10.3892/ol.2024.14272
MLA
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H., Abe, Y., Hasegawa, Y., Hori, S., Tanaka, M., Masugi, Y., Kitago, M., Nishihara, H., Kitagawa, Y."Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid". Oncology Letters 27.4 (2024): 139.
Chicago
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H., Abe, Y., Hasegawa, Y., Hori, S., Tanaka, M., Masugi, Y., Kitago, M., Nishihara, H., Kitagawa, Y."Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid". Oncology Letters 27, no. 4 (2024): 139. https://doi.org/10.3892/ol.2024.14272
Copy and paste a formatted citation
x
Spandidos Publications style
Nakano Y, Shimane G, Nakamura K, Takamatsu R, Aimono E, Yagi H, Abe Y, Hasegawa Y, Hori S, Tanaka M, Tanaka M, et al: Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid. Oncol Lett 27: 139, 2024.
APA
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H. ... Kitagawa, Y. (2024). Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid. Oncology Letters, 27, 139. https://doi.org/10.3892/ol.2024.14272
MLA
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H., Abe, Y., Hasegawa, Y., Hori, S., Tanaka, M., Masugi, Y., Kitago, M., Nishihara, H., Kitagawa, Y."Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid". Oncology Letters 27.4 (2024): 139.
Chicago
Nakano, Y., Shimane, G., Nakamura, K., Takamatsu, R., Aimono, E., Yagi, H., Abe, Y., Hasegawa, Y., Hori, S., Tanaka, M., Masugi, Y., Kitago, M., Nishihara, H., Kitagawa, Y."Next‑generation sequencing to identify genetic mutations in pancreatic cancer using intraoperative peritoneal washing fluid". Oncology Letters 27, no. 4 (2024): 139. https://doi.org/10.3892/ol.2024.14272
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