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Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy

  • Authors:
    • Min Woo Kim
    • Sol Moon
    • Suji Lee
    • Hyojung Lee
    • Young Kim
    • Joon Ye Kim
    • Jee Ye Kim
    • Seung Il Kim
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Yonsei University College of Medicine, Seoul 03722, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 158
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    Published online on: February 16, 2024
       https://doi.org/10.3892/ol.2024.14291
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Abstract

Exosomal microRNAs (miRNAs) are closely related to drug resistance in patients with breast cancer (BC); however, only a few roles of the exosomal miRNA‑target gene networks have been clinically implicated in drug resistance in BC. Therefore, the present study aimed to identify the differential expression of exosomal miRNAs associated with drug resistance and their target mRNAs. In vitro microarray analysis was used to verify differentially expressed miRNAs (DEMs) in drug‑resistant BC. Next, tumor‑derived exosomes (TDEs) were isolated. Furthermore, it was determined whether the candidate drug‑resistant miRNAs were also significant in TDEs, and then putative miRNAs in TDEs were validated in plasma samples from 35 patients with BC (20 patients with BC showing no response and 15 patients with BC showing a complete response). It was confirmed that the combination of five exosomal miRNAs, including miR‑125b‑5p, miR‑146a‑5p, miR‑484, miR‑1246‑5p and miR‑1260b, was effective for predicting therapeutic response to neoadjuvant chemotherapy, with an area under the curve value of 0.95, sensitivity of 75%, and specificity of 95%. Public datasets were analyzed to identify differentially expressed genes (DEGs) related to drug resistance and it was revealed that BAK1, NOVA1, PTGER4, RTKN2, AGO1, CAP1, and ETS1 were the target genes of exosomal miRNAs. Networks between DEMs and DEGs were highly correlated with mitosis, metabolism, drug transport, and immune responses. Consequently, these targets could be used as predictive markers and therapeutic targets for clinical applications to enhance treatment outcomes for patients with BC.
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Copy and paste a formatted citation
Spandidos Publications style
Kim MW, Moon S, Lee S, Lee H, Kim Y, Kim JY, Kim JY and Kim SI: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy. Oncol Lett 27: 158, 2024.
APA
Kim, M.W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J.Y. ... Kim, S.I. (2024). Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy. Oncology Letters, 27, 158. https://doi.org/10.3892/ol.2024.14291
MLA
Kim, M. W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J. Y., Kim, J. Y., Kim, S. I."Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy". Oncology Letters 27.4 (2024): 158.
Chicago
Kim, M. W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J. Y., Kim, J. Y., Kim, S. I."Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy". Oncology Letters 27, no. 4 (2024): 158. https://doi.org/10.3892/ol.2024.14291
Copy and paste a formatted citation
x
Spandidos Publications style
Kim MW, Moon S, Lee S, Lee H, Kim Y, Kim JY, Kim JY and Kim SI: Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy. Oncol Lett 27: 158, 2024.
APA
Kim, M.W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J.Y. ... Kim, S.I. (2024). Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy. Oncology Letters, 27, 158. https://doi.org/10.3892/ol.2024.14291
MLA
Kim, M. W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J. Y., Kim, J. Y., Kim, S. I."Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy". Oncology Letters 27.4 (2024): 158.
Chicago
Kim, M. W., Moon, S., Lee, S., Lee, H., Kim, Y., Kim, J. Y., Kim, J. Y., Kim, S. I."Exploring miRNA‑target gene profiles associated with drug resistance in patients with breast cancer receiving neoadjuvant chemotherapy". Oncology Letters 27, no. 4 (2024): 158. https://doi.org/10.3892/ol.2024.14291
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