Early growth response 1 regulates dual‑specificity protein phosphatase 1 and inhibits cell migration and invasion of tongue squamous cell carcinoma

Zhou,Longxun; Shan,Yuqun; Li,Jun; Li,Min; Meng,Zhen; Guo,Na

doi:10.3892/ol.2024.14373

Early growth response 1 regulates dual‑specificity protein phosphatase 1 and inhibits cell migration and invasion of tongue squamous cell carcinoma

Authors:
- Longxun Zhou
- Yuqun Shan
- Jun Li
- Min Li
- Zhen Meng
- Na Guo
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Affiliations: Department of Stomatology, Liaocheng People's Hospital, Medical School of Liaocheng University, Liaocheng, Shandong 252000, P.R. China, Clinical Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng University, Liaocheng, Shandong 252000, P.R. China, Precision Biomedical Laboratory, Liaocheng People's Hospital, Medical School of Liaocheng University, Liaocheng, Shandong 252000, P.R. China, Biomedical Laboratory, Medical School of Liaocheng University, Liaocheng, Shandong 252000, P.R. China
Published online on: April 3, 2024 https://doi.org/10.3892/ol.2024.14373
Article Number: 240
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumors in the head and neck, and among the OSCCs, tongue squamous cell carcinoma (TSCC) is one of the most common types. Although therapy strategies have recently advanced, the prognosis of TSCC has not substantially improved. Metastasis is one of the main causes of patient mortality in TSCC; therefore, it is necessary to elucidate the mechanism by which TSCC metastasis is regulated. In the present study, the early growth response 1 (Egr‑1) expression in TSCC was analyzed based on GEO datasets and the effect of Egr‑1 in TSCC tumor cell migration and invasion was measured using Transwell assay. By overexpressing dual‑specificity protein phosphatase 1 (DUSP1) in cells with Egr‑1 knockdown using lentivirus infection, the role of DUSP1 in Egr‑1‑regulated TSCC cell migration and invasion was determined. By using luciferase and ChIP assays, the mechanism behind how DUSP1 is regulated by Egr‑1 was detected. In the present study, it was demonstrated that Egr‑1 was downregulated in TSCC and the knockdown of Egr‑1 increased TSCC cell migration and invasion. The expression of Egr‑1 was also correlated with DUSP1. The overexpression of DUSP1 in Egr‑1 knockdown cells, reduced the level of cell migration and invasion. Furthermore, it was demonstrated that knockdown of Egr‑1 inhibited the promoter activity of DUSP1 and the site through which Egr‑1 regulates DUSP1 transcription was identified. In conclusion, the present study demonstrated that Egr‑1 regulates TSCC cell migration and invasion through modulating DUSP1, suggesting the potential of Egr‑1 and DUSP1 as therapy targets for TSCC.

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