Tumor‑suppressive effects of Smad‑ubiquitination regulator 2 in papillary thyroid carcinoma

Luo,Guirong; Zhang,Liting; Zhang,Lihong; Wu,Wenyi; Lin,Jianqing; Shi,Haihong; Yu,Yihuang; Qiu,Weigang; Chen,Jinyan; Ding,Hansen; Chen,Xinyao

doi:10.3892/ol.2024.14396

Tumor‑suppressive effects of Smad‑ubiquitination regulator 2 in papillary thyroid carcinoma

Authors:
- Guirong Luo
- Liting Zhang
- Lihong Zhang
- Wenyi Wu
- Jianqing Lin
- Haihong Shi
- Yihuang Yu
- Weigang Qiu
- Jinyan Chen
- Hansen Ding
- Xinyao Chen
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Affiliations: Department of Thyroid and Breast Surgery, The Second Affiliated Clinical School of Medicine, Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Endocrinology, No. 910th Hospital of The People's Liberation Army Joint Logistics Support Force, Quanzhou, Fujian 362000, P.R. China, Department of General Family Medicine, Jinshang Town Health Center, Quanzhou, Fujian 362000, P.R. China
Published online on: April 10, 2024 https://doi.org/10.3892/ol.2024.14396
Article Number: 263

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Abstract

Smad‑ubiquitination regulator 2 (SMURF2) functions as a homolog of E6AP carboxyl terminus‑type E3 ubiquitin ligase to regulate cell cycle progression and tumor growth factor expression. SMURF2 has been revealed to function as a tumor suppressor in a number of cancers; however, its function in papillary thyroid carcinoma (PTC) remains largely unknown. Therefore, the aim of the present study was to investigate the function of SMURF2 in PTC. Reverse transcription‑quantitative PCR and western blotting were used to detect cellular expression of SMURF2 in vitro. After increasing or inhibiting the expression of SMURF2, MTT was used to detect the effect on tumor cell proliferation and Transwell assays were used to detect the effect on tumor cell migration and invasion. Finally, ELISA was used to detect the effects on glucose and glutamine metabolism in tumor cells and the findings revealed that SMURF2 was downregulated in PTC tissues. Moreover, SMURF2 inhibited the proliferation, invasion and migration of PTC cells, and promoted their apoptosis. Finally, SMURF2 inhibited cell glycolysis and glutaminolysis and affected metabolism in the PTC cell line, TPC‑1. Thus, the findings of the present study suggest that SMURF2 may be a potential target in the treatment of PTC.

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