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Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway

  • Authors:
    • Yuka Kadonosawa
    • Minako Yokoyama
    • Yota Tatara
    • Toshitsugu Fujita
    • Yoshihito Yokoyama
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036‑8562, Japan, Department of Stress Response Science, Center for Advanced Medical Research, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036‑8562, Japan, Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori 036‑8562, Japan
    Copyright: © Kadonosawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 359
    |
    Published online on: June 5, 2024
       https://doi.org/10.3892/ol.2024.14492
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Abstract

High expression of carbonyl reductase 1 (CBR1) protein in ovarian cancer cells inhibits tumor growth and metastasis. However, the underlying mechanism is unknown. To investigate the mechanism by which CBR1 suppresses tumor growth, the present study generated ovarian cancer cells that constitutively overexpress human CBR1 (hCBR1) protein. Ovarian cancer cell lines (OVCAR‑3 and SK‑OV‑3) were transfected with a plasmid encoding hCBR1, followed by selection with G418 to isolate hCBR1‑overexpressing lines. The proliferation rates of hCBR1‑overexpressing cells were then compared with those of negative control and wild‑type cells. Overexpression of hCBR1 led to significant inhibition of proliferation (P<0.05). Subsequently, to investigate changes in intracellular signaling pathways, cellular proteins were extracted and subjected to proteome analysis using liquid chromatography followed by mass spectrometry. There was an inverse correlation between CBR1 protein expression and cell proliferation. In addition, Ingenuity Pathway Analysis of hCBR1‑overexpressing cell lines was performed, which revealed changes in the expression of proteins involved in signaling pathways related to growth regulation. Of these, the eukaryotic translation initiation factor 2 (eIF2) signaling pathway was upregulated most prominently. Thus, alterations in multiple tumor‑related signaling pathways, including eIF2 signaling, may lead to growth suppression. Taken together, the present data may lead to the development of new drugs that target CBR1 and related signaling pathways, thereby improving outcomes for patients with ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Kadonosawa Y, Yokoyama M, Tatara Y, Fujita T and Yokoyama Y: Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway. Oncol Lett 28: 359, 2024.
APA
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., & Yokoyama, Y. (2024). Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway. Oncology Letters, 28, 359. https://doi.org/10.3892/ol.2024.14492
MLA
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., Yokoyama, Y."Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway". Oncology Letters 28.2 (2024): 359.
Chicago
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., Yokoyama, Y."Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway". Oncology Letters 28, no. 2 (2024): 359. https://doi.org/10.3892/ol.2024.14492
Copy and paste a formatted citation
x
Spandidos Publications style
Kadonosawa Y, Yokoyama M, Tatara Y, Fujita T and Yokoyama Y: Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway. Oncol Lett 28: 359, 2024.
APA
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., & Yokoyama, Y. (2024). Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway. Oncology Letters, 28, 359. https://doi.org/10.3892/ol.2024.14492
MLA
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., Yokoyama, Y."Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway". Oncology Letters 28.2 (2024): 359.
Chicago
Kadonosawa, Y., Yokoyama, M., Tatara, Y., Fujita, T., Yokoyama, Y."Overexpression of carbonyl reductase 1 in ovarian cancer cells suppresses proliferation and activates the eIF2 signaling pathway". Oncology Letters 28, no. 2 (2024): 359. https://doi.org/10.3892/ol.2024.14492
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