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Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma

  • Authors:
    • Guohua Yan
    • Nanchang Huang
    • Chaotao Chen
    • Hanji Huang
    • Jianwen Cheng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic and Traumatology Surgery, The First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Reproductive Medicine, Guangxi Maternal and Child Health Hospital, Nanning, Guangxi Zhuang Autonomous Region 530003, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 374
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    Published online on: June 13, 2024
       https://doi.org/10.3892/ol.2024.14507
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Abstract

Circular RNAs (circRNAs) are a subclass of non‑coding RNAs that are important for the regulation of gene expression in eukaryotic organisms. CircRNAs exert various regulatory roles in cancer progression. However, the role of hsa_circ_0064636 in osteosarcoma (OS) remains poorly understood. In the present study, the expression of hsa_circ_0064636 in OS cell lines was measured by reverse transcription‑quantitative PCR (RT‑qPCR). Differentially expressed mRNAs and microRNAs (miRNA or miRs) were screened using mRNA(GSE16088) and miRNA(GSE65071) expression datasets for OS. miRNAs that can potentially interact with hsa_circ_0064636 were predicted using RNAhybrid, TargetScan and miRanda. Subsequently, RNAhybrid, TargetScan, miRanda, miRWalk, miRMap and miRNAMap were used for target gene prediction based on the overlapping miRNAs to construct a circ/miRNA/mRNA interaction network. Target genes were subjected to survival analysis using PROGgeneV2, resulting in a circRNA/miRNA/mRNA interaction sub‑network with prognostic significance. miRNA and circRNA in the subnetwork may also have survival significance, but relevant data are lacking and needs to be further proved.RT‑qPCR demonstrated that hsa_circ_0064636 expression was significantly increased in OS cell lines. miR‑326 and miR‑503‑5p were identified to be target miRNAs of hsa_circ_0064636. Among the target genes obtained from the miR‑326 and miR‑503‑5p screens, ubiquitination factor E4A (UBE4A) and voltage dependent anion channel 1 (VDAC1) were respectively identified to significantly affect prognosis; only miR‑326 targets UBE4A and only miR‑503 targets VDAC1. To conclude, these aforementioned findings suggest that hsa_circ_0064636 may be involved in the development of OS by sponging miR‑503‑5p and miR‑326to inhibit their effects, thereby regulating the expression of VDAC1 and UBE4A.
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Copy and paste a formatted citation
Spandidos Publications style
Yan G, Huang N, Chen C, Huang H and Cheng J: Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma. Oncol Lett 28: 374, 2024.
APA
Yan, G., Huang, N., Chen, C., Huang, H., & Cheng, J. (2024). Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma. Oncology Letters, 28, 374. https://doi.org/10.3892/ol.2024.14507
MLA
Yan, G., Huang, N., Chen, C., Huang, H., Cheng, J."Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma". Oncology Letters 28.2 (2024): 374.
Chicago
Yan, G., Huang, N., Chen, C., Huang, H., Cheng, J."Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma". Oncology Letters 28, no. 2 (2024): 374. https://doi.org/10.3892/ol.2024.14507
Copy and paste a formatted citation
x
Spandidos Publications style
Yan G, Huang N, Chen C, Huang H and Cheng J: Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma. Oncol Lett 28: 374, 2024.
APA
Yan, G., Huang, N., Chen, C., Huang, H., & Cheng, J. (2024). Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma. Oncology Letters, 28, 374. https://doi.org/10.3892/ol.2024.14507
MLA
Yan, G., Huang, N., Chen, C., Huang, H., Cheng, J."Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma". Oncology Letters 28.2 (2024): 374.
Chicago
Yan, G., Huang, N., Chen, C., Huang, H., Cheng, J."Hsa_circ_0064636 regulates voltage dependent anion channel 1/ubiquitination factor E4A through miR‑326/miR‑503‑5 in osteosarcoma". Oncology Letters 28, no. 2 (2024): 374. https://doi.org/10.3892/ol.2024.14507
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