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Proteomic insights into the regulatory function of ARID1A in colon cancer cells

  • Authors:
    • Siripat Aluksanasuwan
    • Keerakarn Somsuan
    • Sasithorn Wanna‑Udom
    • Sittiruk Roytrakul
    • Atthapan Morchang
    • Artitaya Rongjumnong
    • Natthiya Sakulsak
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Mae Fah Luang University, Muang, Chiang Rai 57100, Thailand, Department of Anatomy, Faculty of Medical Science, Naresuan University, Muang, Phitsanulok 65000, Thailand, Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klongluang, Pathum Thani 12120, Thailand
    Copyright: © Aluksanasuwan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 392
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    Published online on: June 21, 2024
       https://doi.org/10.3892/ol.2024.14525
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Abstract

The AT‑rich interacting domain‑containing protein 1A (ARID1A) is a tumor suppressor gene that has been implicated in several cancers, including colorectal cancer (CRC). The present study used a proteomic approach to elucidate the molecular mechanisms of ARID1A in CRC carcinogenesis. Stable ARID1A‑overexpressing SW48 colon cancer cells were established using lentivirus transduction and the successful overexpression of ARID1A was confirmed by western blotting. Label‑free quantitative proteomic analysis using liquid chromatography‑tandem mass spectrometry identified 705 differentially altered proteins in the ARID1A‑overexpressing cells, with 310 proteins significantly increased and 395 significantly decreased compared with empty vector control cells. Gene Ontology enrichment analysis highlighted the involvement of the altered proteins mainly in the Wnt signaling pathway. Western blotting supported these findings, as a decreased protein expression of Wnt target genes, including c‑Myc, transcription factor T cell factor‑1/7 and cyclin D1, were observed in ARID1A‑overexpressing cells. Among the altered proteins involved in the Wnt signaling pathway, the interaction network analysis revealed that ARID1A exhibited a direct interaction with E3 ubiquitin‑protein ligase zinc and ring finger 3 (ZNRF3), a negative regulator of the Wnt signaling pathway. Further analyses using the The Cancer Genome Atlas colon adenocarcinoma public dataset revealed that ZNRF3 expression significantly impacted the overall survival of patients with CRC and was positively correlated with ARID1A expression. Finally, an increased level of ZNRF3 in ARID1A‑overexpressing cells was confirmed by western blotting. In conclusion, the findings of the present study suggest that ARID1A negatively regulates the Wnt signaling pathway through ZNRF3, which may contribute to CRC carcinogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Aluksanasuwan S, Somsuan K, Wanna‑Udom S, Roytrakul S, Morchang A, Rongjumnong A and Sakulsak N: Proteomic insights into the regulatory function of ARID1A in colon cancer cells. Oncol Lett 28: 392, 2024.
APA
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., & Sakulsak, N. (2024). Proteomic insights into the regulatory function of ARID1A in colon cancer cells. Oncology Letters, 28, 392. https://doi.org/10.3892/ol.2024.14525
MLA
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., Sakulsak, N."Proteomic insights into the regulatory function of ARID1A in colon cancer cells". Oncology Letters 28.2 (2024): 392.
Chicago
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., Sakulsak, N."Proteomic insights into the regulatory function of ARID1A in colon cancer cells". Oncology Letters 28, no. 2 (2024): 392. https://doi.org/10.3892/ol.2024.14525
Copy and paste a formatted citation
x
Spandidos Publications style
Aluksanasuwan S, Somsuan K, Wanna‑Udom S, Roytrakul S, Morchang A, Rongjumnong A and Sakulsak N: Proteomic insights into the regulatory function of ARID1A in colon cancer cells. Oncol Lett 28: 392, 2024.
APA
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., & Sakulsak, N. (2024). Proteomic insights into the regulatory function of ARID1A in colon cancer cells. Oncology Letters, 28, 392. https://doi.org/10.3892/ol.2024.14525
MLA
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., Sakulsak, N."Proteomic insights into the regulatory function of ARID1A in colon cancer cells". Oncology Letters 28.2 (2024): 392.
Chicago
Aluksanasuwan, S., Somsuan, K., Wanna‑Udom, S., Roytrakul, S., Morchang, A., Rongjumnong, A., Sakulsak, N."Proteomic insights into the regulatory function of ARID1A in colon cancer cells". Oncology Letters 28, no. 2 (2024): 392. https://doi.org/10.3892/ol.2024.14525
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