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A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors

  • Authors:
    • Tsunglin Liu
    • Chung-Liang Ho
    • Yan-Jhen Chen
    • Pin-Jun Chen
    • Wan-Li Chen
    • Chung-Ta Lee
    • Nan-Haw Chow
    • Wenya Huang
    • Yi-Lin Chen
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan 701, Taiwan, R.O.C., Molecular Diagnosis Laboratory, Department of Pathology, National Cheng Kung University Hospital, Tainan 704, Taiwan, R.O.C., Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan, R.O.C., Department of Laboratory Medicine, Center for Precision Medicine, China Medical University Hospital, Taichung 404, Taiwan, R.O.C.
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 445
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    Published online on: July 22, 2024
       https://doi.org/10.3892/ol.2024.14578
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Abstract

Microsatellite instability (MSI) status is a prognostic biomarker for immunotherapy in certain types of cancers, such as colorectal cancers (CRCs) and endometrial cancers (ECs). Tumors that are categorized as having high MSI (MSI‑H) express high levels of neoantigens for immune recognition. The typical MSI test measures the length of short mononucleotide repeats (SMR) poly(A) 21‑27; however, a limitation of this test is the difficulty in determining the shift size, particularly in endometrial cancer. To investigate an MSI detection assay with improved performance, the present study analyzed the use of poly(A) 40‑44 mononucleotide repeats to detect the MSI status of 100 patients with either CRC (n=50) or EC (n=50). Capillary electrophoresis was used to evaluate five long mononucleotide repeat (LMR) markers, including poly(A) 40‑A, 40‑B, 40‑C, 40‑D and 44. The concordance rate of the LMR‑MSI assay compared with an immunohistochemistry MSI detection assay was 96.0 and 95.1% for CRCs and ECs respectively, with the detection limit of the LMR‑MSI assay demonstrated to be 2.5% MSI‑H in HCT116 colorectal carcinoma cell lines. The LMR‑MSI assay yielded a 95.1% concordance rate in ECs compared with that in the SMR‑MSI test (87.8%). The LMR‑MSI test identified a significantly higher mean shift size (13 bp) in MSI‑H tumors compared with the SMR‑MSI test (10 bp), in both EC and CRC tissue samples. Together, the present study suggested that the LMR‑MSI test could potentially be a sensitive and practical technology for molecular laboratory testing, particularly in the use of immunotherapy for patients with CRCs and ECs.
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1 

Richard GF, Kerrest A and Dujon B: Comparative genomics and molecular dynamics of DNA repeats in eukaryotes. Microbiol Mol Biol Rev. 72:686–727. 2008. View Article : Google Scholar

2 

Lynch HT, Shaw MW, Magnuson CW, Larsen AL and Krush AJ: Hereditary factors in cancer. Study of two large midwestern kindreds. Arch Intern Med. 117:206–212. 1966. View Article : Google Scholar

3 

Dean DA, Wadl PA, Hadziabdic D, Wang X and Trigiano RN: Analyzing microsatellites using the QIAxcel system. Methods Mol Biol. 1006:223–243. 2013. View Article : Google Scholar

4 

Li K, Luo H, Huang L, Luo H and Zhu X: Microsatellite instability: A review of what the oncologist should know. Cancer Cell Int. 20:162020. View Article : Google Scholar

5 

Fan H and Chu JY: A brief review of short tandem repeat mutation. Genomics Proteomics Bioinformatics. 5:7–14. 2007. View Article : Google Scholar

6 

Roudko V, Cimen Bozkus C, Greenbaum B, Lucas A, Samstein R and Bhardwaj N: Lynch Syndrome and MSI-H Cancers: From Mechanisms to ‘Off-The-Shelf’ Cancer Vaccines. Front Immunol. 12:7578042021. View Article : Google Scholar

7 

Reyes GX, Schmidt TT, Kolodner RD and Hombauer H: New insights into the mechanism of DNA mismatch repair. Chromosoma. 124:443–462. 2015. View Article : Google Scholar

8 

Tamura K, Kaneda M, Futagawa M, Takeshita M, Kim S, Nakama M, Kawashita N and Tatsumi-Miyajima J: Genetic and genomic basis of the mismatch repair system involved in Lynch syndrome. Int J Clin Oncol. 24:999–1011. 2019. View Article : Google Scholar

9 

Hause RJ, Pritchard CC, Shendure J and Salipante SJ: Classification and characterization of microsatellite instability across 18 cancer types. Nat Med. 22:1342–1350. 2016. View Article : Google Scholar

10 

Le DT, Uram JN, Wang H, Bartlett BR, Kemberling H, Eyring AD, Skora AD, Luber BS, Azad NS, Laheru D, et al: PD-1 blockade in tumors with mismatch-repair deficiency. N Engl J Med. 372:2509–2520. 2015. View Article : Google Scholar

11 

McMeekin DS, Tritchler DL, Cohn DE, Mutch DG, Lankes HA, Geller MA, Powell MA, Backes FJ, Landrum LM, Zaino R, et al: Clinicopathologic significance of mismatch repair defects in endometrial cancer: An NRG Oncology/Gynecologic oncology group study. J Clin Oncol. 34:3062–3068. 2016. View Article : Google Scholar

12 

Bonneville R, Krook MA, Kautto EA, Miya J, Wing MR, Chen HZ, Reeser JW, Yu L and Roychowdhury S: Landscape of microsatellite instability across 39 cancer types. JCO Precis Oncol. 2017.PO.17.00073, 2017. View Article : Google Scholar

13 

Green AK, Feinberg J and Makker V: A Review of immune checkpoint blockade therapy in endometrial cancer. Am Soc Clin Oncol Educ Book. 40:1–7. 2020.

14 

Boland CR and Goel A: Microsatellite instability in colorectal cancer. Gastroenterology. 138:2073–2087.e3. 2010. View Article : Google Scholar

15 

Petrelli F, Ghidini M, Cabiddu M, Pezzica E, Corti D, Turati L, Costanzo A, Varricchio A, Ghidini A, Barni S, et al: Microsatellite instability and survival in stage ii colorectal cancer: A systematic review and meta-analysis. Anticancer Res. 39:6431–6441. 2019. View Article : Google Scholar

16 

Lin A, Zhang J and Luo P: Crosstalk Between the MSI status and tumor microenvironment in colorectal cancer. Front Immunol. 11:20392020. View Article : Google Scholar

17 

Makker V, Colombo N, Casado Herraez A, Santin AD, Colomba E, Miller DS, Fujiwara K, Pignata S, Baron-Hay S, Ray-Coquard I, et al: Lenvatinib plus Pembrolizumab for advanced endometrial cancer. N Engl J Med. 386:437–448. 2022. View Article : Google Scholar

18 

Le DT, Durham JN, Smith KN, Wang H, Bartlett BR, Aulakh LK, Lu S, Kemberling H, Wilt C, Luber BS, et al: Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade. Science. 357:409–413. 2017. View Article : Google Scholar

19 

Lenz HJ, Van Cutsem E, Luisa Limon M, Wong KYM, Hendlisz A, Aglietta M, Garcia-Alfonso P, Neyns B, Luppi G, Cardin DB, et al: First-Line nivolumab plus Low-Dose ipilimumab for microsatellite Instability-High/Mismatch repair-deficient metastatic colorectal cancer: The phase II CheckMate 142 study. J Clin Oncol. 40:161–170. 2022. View Article : Google Scholar

20 

Green AK, Feinberg J and Makker V: A review of immune checkpoint blockade therapy in endometrial cancer. Am Soc Clin Oncol Educ Book. 40:1–7. 2020.

21 

Narayanan S, Kawaguchi T, Peng X, Qi Q, Liu S, Yan L and Takabe K: Tumor infiltrating lymphocytes and macrophages improve survival in microsatellite unstable colorectal cancer. Sci Rep. 9:134552019. View Article : Google Scholar

22 

Diao Z, Han Y, Chen Y, Zhang R and Li J: The clinical utility of microsatellite instability in colorectal cancer. Crit Rev Oncol Hematol. 157:1031712021. View Article : Google Scholar

23 

Pal A, Greenblatt HM and Levy Y: Prerecognition diffusion mechanism of human DNA mismatch repair proteins along DNA: Msh2-Msh3 versus Msh2-Msh6. Biochemistry. 59:4822–4832. 2020. View Article : Google Scholar

24 

Marsischky GT, Filosi N, Kane MF and Kolodner R: Redundancy of saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair. Genes Dev. 10:407–420. 1996. View Article : Google Scholar

25 

Hewish M, Lord CJ, Martin SA, Cunningham D and Ashworth A: Mismatch repair deficient colorectal cancer in the era of personalized treatment. Nat Rev Clin Oncol. 7:197–208. 2010. View Article : Google Scholar

26 

Luchini C, Bibeau F, Ligtenberg MJL, Singh N, Nottegar A, Bosse T, Miller R, Riaz N, Douillard JY, Andre F, et al: ESMO recommendations on microsatellite instability testing for immunotherapy in cancer, and its relationship with PD-1/PD-L1 expression and tumour mutational burden: A systematic review-based approach. Ann Oncol. 30:1232–1243. 2019. View Article : Google Scholar

27 

Benson AB, Venook AP, Al-Hawary MM, Arain MA, Chen YJ, Ciombor KK, Cohen S, Cooper HS, Deming D, Farkas L, et al: Colon cancer, version 2.2021, NCCN clinical practice guidelines in oncology. J Natl Compr Canc Netw. 19:329–359. 2021. View Article : Google Scholar

28 

Funkhouser WK Jr, Lubin IM, Monzon FA, Zehnbauer BA, Evans JP, Ogino S and Nowak JA: Relevance, pathogenesis, and testing algorithm for mismatch repair-defective colorectal carcinomas: A report of the association for molecular pathology. J Mol Diagn. 14:91–103. 2012. View Article : Google Scholar

29 

Cicek MS, Lindor NM, Gallinger S, Bapat B, Hopper JL, Jenkins MA, Young J, Buchanan D, Walsh MD, Le Marchand L, et al: Quality assessment and correlation of microsatellite instability and immunohistochemical markers among population- and clinic-based colorectal tumors results from the colon cancer family registry. J Mol Diagn. 13:271–281. 2011. View Article : Google Scholar

30 

Hechtman JF, Rana S, Middha S, Stadler ZK, Latham A, Benayed R, Soslow R, Ladanyi M, Yaeger R, Zehir A, et al: Retained mismatch repair protein expression occurs in approximately 6% of microsatellite instability-high cancers and is associated with missense mutations in mismatch repair genes. Mod Pathol. 33:871–879. 2020. View Article : Google Scholar

31 

Cohen R, Hain E, Buhard O, Guilloux A, Bardier A, Kaci R, Bertheau P, Renaud F, Bibeau F, Flejou JF, et al: Association of primary resistance to immune checkpoint inhibitors in metastatic colorectal cancer with misdiagnosis of microsatellite instability or mismatch repair deficiency status. JAMA Oncol. 5:551–555. 2019. View Article : Google Scholar

32 

Huang W, Ho CL, Lee CT, Chen WL, Yang SC, Chow NH and Chen YL: High concordance rate of capillary electrophoresis workflow for microsatellite instability analysis and mismatch repair (MMR) immunostaining in colorectal carcinoma. PLoS One. 18:e02842272023. View Article : Google Scholar

33 

Murphy KM, Zhang S, Geiger T, Hafez MJ, Bacher J, Berg KD and Eshleman JR: Comparison of the microsatellite instability analysis system and the Bethesda panel for the determination of microsatellite instability in colorectal cancers. J Mol Diagn. 8:305–311. 2006. View Article : Google Scholar

34 

Vikas P, Messersmith H, Compton C, Sholl L, Broaddus RR, Davis A, Estevez-Diz M, Garje R, Konstantinopoulos PA, Leiser A, et al: Mismatch repair and microsatellite instability testing for immune checkpoint inhibitor therapy: ASCO endorsement of college of american pathologists guideline. J Clin Oncol. 41:1943–1948. 2023. View Article : Google Scholar

35 

Taieb J, Svrcek M, Cohen R, Basile D, Tougeron D and Phelip JM: Deficient mismatch repair/microsatellite unstable colorectal cancer: Diagnosis, prognosis and treatment. Eur J Cancer. 175:136–157. 2022. View Article : Google Scholar

36 

Rodriguez-Bigas MA, Boland CR, Hamilton SR, Henson DE, Jass JR, Khan PM, Lynch H, Perucho M, Smyrk T, Sobin L, et al: A National cancer institute workshop on hereditary nonpolyposis colorectal cancer syndrome: Meeting highlights and Bethesda guidelines. J Natl Cancer Inst. 89:1758–1762. 1997. View Article : Google Scholar

37 

Umar A, Boland CR, Terdiman JP, Syngal S, de la Chapelle A, Rüschoff J, Fishel R, Lindor NM, Burgart LJ, Hamelin R, et al: Revised Bethesda Guidelines for hereditary nonpolyposis colorectal cancer (Lynch syndrome) and microsatellite instability. J Natl Cancer Inst. 96:261–268. 2004. View Article : Google Scholar

38 

Bacher JW, Flanagan LA, Smalley RL, Nassif NA, Burgart LJ, Halberg RB, Megid WM and Thibodeau SN: Development of a fluorescent multiplex assay for detection of MSI-High tumors. Dis Markers. 20:237–250. 2004. View Article : Google Scholar

39 

Goel A, Nagasaka T, Hamelin R and Boland CR: An optimized pentaplex PCR for detecting DNA mismatch repair-deficient colorectal cancers. PLoS One. 5:e93932010. View Article : Google Scholar

40 

Wang Y, Shi C, Eisenberg R and Vnencak-Jones CL: Differences in microsatellite instability profiles between endometrioid and colorectal cancers: A potential cause for False-Negative results? J Mol Diagn. 19:57–64. 2017. View Article : Google Scholar

41 

Do H and Dobrovic A: Sequence artifacts in DNA from formalin-fixed tissues: Causes and strategies for minimization. Clin Chem. 61:64–71. 2015. View Article : Google Scholar

42 

McDonough SJ, Bhagwate A, Sun Z, Wang C, Zschunke M, Gorman JA, Kopp KJ and Cunningham JM: Use of FFPE-derived DNA in next generation sequencing: DNA extraction methods. PLoS One. 14:e02114002019. View Article : Google Scholar

43 

Wu S, Liu X, Wang J, Zhou W, Guan M, Liu Y, Pang J, Lu T, Zhou L, Shi X, et al: DNA Mismatch repair deficiency detection in colorectal cancer by a new microsatellite instability analysis system. Interdiscip Sci. 12:145–154. 2020. View Article : Google Scholar

44 

Amin MB, Greene FL, Edge SB, Compton CC, Gershenwald JE, Brookland RK, Meyer L, Gress DM, Byrd DR and Winchester DP: The eighth edition AJCC cancer staging manual: Continuing to build a bridge from a population-based to a more ‘personalized’ approach to cancer staging. CA Cancer J Clin. 67:93–99. 2017. View Article : Google Scholar

45 

Lee CT, Chow NH, Chen YL, Ho CL, Yeh YM, Lin SC, Lin PC, Lin BW, Chu CA, Tsai HW, et al: Clinicopathological features of mismatch repair protein expression patterns in colorectal cancer. Pathol Res Pract. 217:1532882021. View Article : Google Scholar

46 

O'Leary NA, Wright MW, Brister JR, Ciufo S, Haddad D, McVeigh R, Rajput B, Robbertse B, Smith-White B, Ako-Adjei D, et al: Reference sequence (RefSeq) database at NCBI: Current status, taxonomic expansion, and functional annotation. Nucleic Acids Res. 44:D733–D745. 2016. View Article : Google Scholar

47 

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S and Madden TL: Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 13:1342012. View Article : Google Scholar

48 

Akoglu H: User's guide to sample size estimation in diagnostic accuracy studies. Turk J Emerg Med. 22:177–185. 2022. View Article : Google Scholar

49 

Yu F, Makrigiorgos A, Leong KW and Makrigiorgos GM: Sensitive detection of microsatellite instability in tissues and liquid biopsies: Recent developments and updates. Comput Struct Biotechnol J. 19:4931–4940. 2021. View Article : Google Scholar

50 

Liu HX, Zhou XL, Liu T, Werelius B, Lindmark G, Dahl N and Lindblom A: The role of hMLH3 in familial colorectal cancer. Cancer Res. 63:1894–1899. 2003.

51 

Kawakami H, Zaanan A and Sinicrope FA: Microsatellite instability testing and its role in the management of colorectal cancer. Curr Treat Options Oncol. 16:302015. View Article : Google Scholar

52 

Jaffrelot M, Farés N, Brunac AC, Laurenty AP, Danjoux M, Grand D, Icher S, Meilleroux J, Mery E, Buscail E, et al: An unusual phenotype occurs in 15% of mismatch repair-deficient tumors and is associated with non-colorectal cancers and genetic syndromes. Mod Pathol. 35:427–437. 2022. View Article : Google Scholar

53 

Wang C, Zhang L, Vakiani E and Shia J: Detecting mismatch repair deficiency in solid neoplasms: Immunohistochemistry, microsatellite instability, or both? Mod Pathol. 35:1515–1528. 2022. View Article : Google Scholar

54 

Bao F, Panarelli NC, Rennert H, Sherr DL and Yantiss RK: Neoadjuvant therapy induces loss of MSH6 expression in colorectal carcinoma. Am J Surg Pathol. 34:1798–1804. 2010. View Article : Google Scholar

55 

Kuan SF, Ren B, Brand R, Dudley B and Pai RK: Neoadjuvant therapy in microsatellite-stable colorectal carcinoma induces concomitant loss of MSH6 and Ki-67 expression. Hum Pathol. 63:33–39. 2017. View Article : Google Scholar

56 

Guyot D'Asnières De Salins A, Tachon G, Cohen R, Karayan-Tapon L, Junca A, Frouin E, Godet J, Evrard C, Randrian V, Duval A, et al: Discordance between immunochemistry of mismatch repair proteins and molecular testing of microsatellite instability in colorectal cancer. ESMO Open. 6:1001202021. View Article : Google Scholar

57 

Bacher JW, Sievers CK, Albrecht DM, Grimes IC, Weiss JM, Matkowskyj KA, Agni RM, Vyazunova I, Clipson L, Storts DR, et al: Improved detection of microsatellite instability in early colorectal lesions. PLoS One. 10:e01327272015. View Article : Google Scholar

58 

Lin JH, Chen S, Pallavajjala A, Guedes LB, Lotan TL, Bacher JW and Eshleman JR: Validation of long mononucleotide repeat markers for detection of microsatellite instability. J Mol Diagn. 24:144–157. 2022. View Article : Google Scholar

59 

Turner EH, Dickerson JA, Ramsay LM, Swearingen KE, Wojcik R and Dovichi NJ: Reaction of fluorogenic reagents with proteins III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503. J Chromatogr A. 1194:253–256. 2008. View Article : Google Scholar

60 

Zwaenepoel K, Holmgaard Duelund J, De Winne K, Maes V, Weyn C, Lambin S, Dendooven R, Broeckx G, Steiniche T and Pauwels P: Clinical performance of the idylla MSI test for a rapid assessment of the DNA microsatellite status in human colorectal cancer. J Mol Diagn. 22:386–395. 2020. View Article : Google Scholar

61 

Siemanowski J, Schomig-Markiefka B, Buhl T, Haak A, Siebolts U, Dietmaier W, Arens N, Pauly N, Ataseven B, Buttner R, et al: Managing difficulties of microsatellite instability testing in endometrial Cancer-Limitations and advantages of four different PCR-Based approaches. Cancers (Basel). 13:12682021. View Article : Google Scholar

62 

Cancer Genome Atlas Research Network, . Comprehensive molecular characterization of gastric adenocarcinoma. Nature. 513:202–209. 2014. View Article : Google Scholar

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Spandidos Publications style
Liu T, Ho C, Chen Y, Chen P, Chen W, Lee C, Chow N, Huang W and Chen Y: A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors. Oncol Lett 28: 445, 2024.
APA
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C. ... Chen, Y. (2024). A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors. Oncology Letters, 28, 445. https://doi.org/10.3892/ol.2024.14578
MLA
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C., Chow, N., Huang, W., Chen, Y."A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors". Oncology Letters 28.3 (2024): 445.
Chicago
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C., Chow, N., Huang, W., Chen, Y."A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors". Oncology Letters 28, no. 3 (2024): 445. https://doi.org/10.3892/ol.2024.14578
Copy and paste a formatted citation
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Spandidos Publications style
Liu T, Ho C, Chen Y, Chen P, Chen W, Lee C, Chow N, Huang W and Chen Y: A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors. Oncol Lett 28: 445, 2024.
APA
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C. ... Chen, Y. (2024). A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors. Oncology Letters, 28, 445. https://doi.org/10.3892/ol.2024.14578
MLA
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C., Chow, N., Huang, W., Chen, Y."A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors". Oncology Letters 28.3 (2024): 445.
Chicago
Liu, T., Ho, C., Chen, Y., Chen, P., Chen, W., Lee, C., Chow, N., Huang, W., Chen, Y."A pilot study on the detection of microsatellite instability using long mononucleotide repeats in solid tumors". Oncology Letters 28, no. 3 (2024): 445. https://doi.org/10.3892/ol.2024.14578
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