Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2024 Volume 28 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2024 Volume 28 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells

  • Authors:
    • Bo Zheng
    • Kuifu Chen
    • Xin Liu
    • Zhenghua Wan
    • Yulong Wu
    • Liming Xu
    • Jiguang Xiao
    • Jinqu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Fifth Hospital of Xiamen City, Xiamen, Fujian 361101, P.R. China, Department of Radiology, The Second Affiliated Hospital of Xiamen Medical College, Xiamen, Fujian 361000, P.R. China
    Copyright: © Zheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 453
    |
    Published online on: July 23, 2024
       https://doi.org/10.3892/ol.2024.14585
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Prostate cancer remains the most prevalent malignancy diagnosed in men worldwide. Epithelial cell transforming sequence 2 (ECT2) is an oncogene involved in the progression of human tumors. The present study aimed to explore the involvement of ECT2 in prostate cancer and its participation in the malignant progression of prostate cancer. ECT2 expression in prostate cancer cell lines was examined via reverse transcription‑quantitative PCR and western blotting. The effects of knockdown of ECT2 expression in PC‑3 cells on cellular biological behaviors, including proliferation, migration and invasion, were examined using Cell Counting Kit‑8, colony formation, wound healing and Transwell assays. The glycolysis level was determined based on the lactate release, glucose uptake, oxygen consumption rate and extracellular acidification rate. The binding relationship between ECT2 and ETS1 was verified using luciferase reporter and chromatin immunoprecipitation assays. The results indicated that ECT2 was highly expressed in prostate cancer cell lines. Knockdown of ECT2 expression could inhibit cell proliferation, migration, invasion and glycolysis. In addition, the transcription factor ETS1 could directly bind to the ECT2 promoter and positively regulate ECT2 expression. These data were combined with the results of rescue experiments and demonstrated that the inhibitory effects of the knockdown of ECT2 expression on the malignant behavior and glycolysis of prostate cancer cells were partially reversed by ETS1 overexpression. In conclusion, ETS1 induced transcriptional upregulation of ECT2 and enhanced the malignant biological behaviors of prostate cancer cells, thereby promoting the progression of prostate cancer. This evidence provides a theoretical basis for the treatment of prostate cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Adamaki M and Zoumpourlis V: Prostate cancer biomarkers: From diagnosis to prognosis and precision-guided therapeutics. Pharmacol Ther. 228:1079322021. View Article : Google Scholar : PubMed/NCBI

2 

Uhr A, Glick L and Gomella LG: An overview of biomarkers in the diagnosis and management of prostate cancer. Can J Urol. 27((S3)): 24–27. 2020.PubMed/NCBI

3 

Siegel RL, Miller KD, Wagle NS and Jemal A: Cancer statistics, 2023. CA Cancer J Clin. 73:17–48. 2023. View Article : Google Scholar : PubMed/NCBI

4 

Culp MB, Soerjomataram I, Efstathiou JA, Bray F and Jemal A: Recent global patterns in prostate cancer incidence and mortality rates. Eur Urol. 77:38–52. 2020. View Article : Google Scholar : PubMed/NCBI

5 

Howard N, Clementino M, Kim D, Wang L, Verma A, Shi X, Zhang Z and DiPaola RS: New developments in mechanisms of prostate cancer progression. Semin Cancer Biol. 57:111–116. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Bhoir S and De Benedetti A: Targeting prostate cancer, the ‘tousled way’. Int J Mol Sci. 24:111002023. View Article : Google Scholar : PubMed/NCBI

7 

Sehrawat A, Gao L, Wang Y, Bankhead A III, McWeeney SK, King CJ, Schwartzman J, Urrutia J, Bisson WH, Coleman DJ, et al: LSD1 activates a lethal prostate cancer gene network independently of its demethylase function. Proc Natl Acad Sci USA. 115:E4179–E4188. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Fields AP and Justilien V: The guanine nucleotide exchange factor (GEF) Ect2 is an oncogene in human cancer. Adv Enzyme Regul. 50:190–200. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Saito S, Liu XF, Kamijo K, Raziuddin R, Tatsumoto T, Okamoto I, Chen X, Lee CC, Lorenzi MV, Ohara N and Miki T: Deregulation and mislocalization of the cytokinesis regulator ECT2 activate the Rho signaling pathways leading to malignant transformation. J Biol Chem. 279:7169–7179. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Schneid S, Wolff F, Buchner K, Bertram N, Baygun S, Barbosa P, Mangal S and Zanin E: The BRCT domains of ECT2 have distinct functions during cytokinesis. Cell Rep. 34:1088052021. View Article : Google Scholar : PubMed/NCBI

11 

Cook DR, Kang M, Martin TD, Galanko JA, Loeza GH, Trembath DG, Justilien V, Pickering KA, Vincent DF, Jarosch A, et al: Aberrant expression and subcellular localization of ECT2 drives colorectal cancer progression and growth. Cancer Res. 82:90–104. 2022. View Article : Google Scholar : PubMed/NCBI

12 

Zhang H, Geng Y, Sun C and Yu J: Upregulation of ECT2 predicts adverse clinical outcomes and increases 5-fluorouracil resistance in gastric cancer patients. J Oncol. 2021:21028902021.PubMed/NCBI

13 

Sun BY, Wei QQ, Liu CX, Zhang L, Luo G, Li T and Lü MH: ECT2 promotes proliferation and metastasis of esophageal squamous cell carcinoma via the RhoA-ERK signaling pathway. Eur Rev Med Pharmacol Sci. 24:7991–8000. 2020.PubMed/NCBI

14 

Yi M, Zhang D, Song B, Zhao B, Niu M, Wu Y, Dai Z and Wu K: Increased expression of ECT2 predicts the poor prognosis of breast cancer patients. Exp Hematol Oncol. 11:1072022. View Article : Google Scholar : PubMed/NCBI

15 

Guo Z, Chen X, Du T, Zhu D, Lai Y, Dong W, Wu W, Lin C, Liu L and Huang H: Elevated levels of epithelial cell transforming sequence 2 predicts poor prognosis for prostate cancer. Med Oncol. 34:132017. View Article : Google Scholar : PubMed/NCBI

16 

Chandrashekar DS, Karthikeyan SK, Korla PK, Patel H, Shovon AR, Athar M, Netto GJ, Qin ZS, Kumar S, Manne U, et al: UALCAN: An update to the integrated cancer data analysis platform. Neoplasia. 25:18–27. 2022. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Zhang Y and Weinberg RA: Epithelial-to-mesenchymal transition in cancer: Complexity and opportunities. Front Med. 12:361–373. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Cai K, Chen S, Zhu C, Li L, Yu C, He Z and Sun C: FOXD1 facilitates pancreatic cancer cell proliferation, invasion, and metastasis by regulating GLUT1-mediated aerobic glycolysis. Cell Death Dis. 13:7652022. View Article : Google Scholar : PubMed/NCBI

20 

Vander Heiden MG, Cantley LC and Thompson CB: Understanding the Warburg effect: The metabolic requirements of cell proliferation. Science. 324:1029–1033. 2009. View Article : Google Scholar : PubMed/NCBI

21 

DeBerardinis RJ, Lum JJ, Hatzivassiliou G and Thompson CB: The biology of cancer: Metabolic reprogramming fuels cell growth and proliferation. Cell Metab. 7:11–20. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Chelakkot C, Chelakkot VS, Shin Y and Song K: Modulating glycolysis to improve cancer therapy. Int J Mol Sci. 24:26062023. View Article : Google Scholar : PubMed/NCBI

23 

Li L, Liang Y, Kang L, Liu Y, Gao S, Chen S, Li Y, You W, Dong Q, Hong T, et al: Transcriptional regulation of the warburg effect in cancer by SIX1. Cancer Cell. 33:368–385. e72018. View Article : Google Scholar : PubMed/NCBI

24 

Wu Z, Wu J, Zhao Q, Fu S and Jin J: Emerging roles of aerobic glycolysis in breast cancer. Clin Transl Oncol. 22:631–646. 2020. View Article : Google Scholar : PubMed/NCBI

25 

Xu D, Wang Y, Wu J, Zhang Z, Chen J, Xie M, Tang R, Chen C, Chen L, Lin S, et al: ECT2 overexpression promotes the polarization of tumor-associated macrophages in hepatocellular carcinoma via the ECT2/PLK1/PTEN pathway. Cell Death Dis. 12:1622021. View Article : Google Scholar : PubMed/NCBI

26 

Ren K, Zhou D, Wang M, Li E, Hou C, Su Y, Zou Q, Zhou P and Liu X: RACGAP1 modulates ECT2-Dependent mitochondrial quality control to drive breast cancer metastasis. Exp Cell Res. 400:1124932021. View Article : Google Scholar : PubMed/NCBI

27 

Zhi T, Jiang K, Xu X, Yu T, Zhou F, Wang Y, Liu N and Zhang J: ECT2/PSMD14/PTTG1 axis promotes the proliferation of glioma through stabilizing E2F1. Neuro Oncol. 21:462–473. 2019. View Article : Google Scholar : PubMed/NCBI

28 

Wang S, Linde MH, Munde M, Carvalho VD, Wilson WD and Poon GM: Mechanistic heterogeneity in site recognition by the structurally homologous DNA-binding domains of the ETS family transcription factors Ets-1 and PU.1. J Biol Chem. 289:21605–21616. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Geng XD, Wang WW, Feng Z, Liu R, Cheng XL, Shen WJ, Dong ZY, Cai GY, Chen XM, Hong Q and Wu D: Identification of key genes and pathways in diabetic nephropathy by bioinformatics analysis. J Diabetes Investig. 10:972–984. 2019. View Article : Google Scholar : PubMed/NCBI

30 

Chakraborty S and Banerjee S: Multidimensional computational study to understand non-coding RNA interactions in breast cancer metastasis. Sci Rep. 13:157712023. View Article : Google Scholar : PubMed/NCBI

31 

Yang F, Liu Y and Wang P, Wang X, Chu M and Wang P: Mutation of the ETS1 3′UTR interacts with miR-216a-3p to regulate granulosa cell apoptosis in sheep. Theriogenology. 210:133–142. 2023. View Article : Google Scholar : PubMed/NCBI

32 

Dittmer J: The role of the transcription factor Ets1 in carcinoma. Semin Cancer Biol. 35:20–38. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Chen Y, Peng C, Chen J, Chen D, Yang B, He B, Hu W, Zhang Y, Liu H, Dai L, et al: WTAP facilitates progression of hepatocellular carcinoma via m6A-HuR-dependent epigenetic silencing of ETS1. Mol Cancer. 18:1272019. View Article : Google Scholar : PubMed/NCBI

34 

Smith AM, Findlay VJ, Bandurraga SG, Kistner-Griffin E, Spruill LS, Liu A, Golshayan AR and Turner DP: ETS1 transcriptional activity is increased in advanced prostate cancer and promotes the castrate-resistant phenotype. Carcinogenesis. 33:572–580. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Mao S, Zhang W, Yang F, Guo Y, Wang H, Wu Y, Wang R, Maskey N, Zheng Z, Li C, et al: Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA. J Exp Clin Cancer Res. 40:3362021. View Article : Google Scholar : PubMed/NCBI

36 

Xu W, Zeng F, Li S, Li G, Lai X, Wang QJ and Deng F: Crosstalk of protein kinase C ε with Smad2/3 promotes tumor cell proliferation in prostate cancer cells by enhancing aerobic glycolysis. Cell Mol Life Sci. 75:4583–4598. 2018. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zheng B, Chen K, Liu X, Wan Z, Wu Y, Xu L, Xiao J and Chen J: Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells. Oncol Lett 28: 453, 2024.
APA
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L. ... Chen, J. (2024). Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells. Oncology Letters, 28, 453. https://doi.org/10.3892/ol.2024.14585
MLA
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L., Xiao, J., Chen, J."Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells". Oncology Letters 28.4 (2024): 453.
Chicago
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L., Xiao, J., Chen, J."Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells". Oncology Letters 28, no. 4 (2024): 453. https://doi.org/10.3892/ol.2024.14585
Copy and paste a formatted citation
x
Spandidos Publications style
Zheng B, Chen K, Liu X, Wan Z, Wu Y, Xu L, Xiao J and Chen J: Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells. Oncol Lett 28: 453, 2024.
APA
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L. ... Chen, J. (2024). Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells. Oncology Letters, 28, 453. https://doi.org/10.3892/ol.2024.14585
MLA
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L., Xiao, J., Chen, J."Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells". Oncology Letters 28.4 (2024): 453.
Chicago
Zheng, B., Chen, K., Liu, X., Wan, Z., Wu, Y., Xu, L., Xiao, J., Chen, J."Transcription factor ETS1‑mediated ECT2 expression promotes the malignant behavior of prostate cancer cells". Oncology Letters 28, no. 4 (2024): 453. https://doi.org/10.3892/ol.2024.14585
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team