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Article

Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway

  • Authors:
    • Chunyan Wu
    • Wei Ge
    • Yun Wu
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Baotou Central Hospital, Baotou, Inner Mongolia Autonomous Region 014040, P.R. China
  • Article Number: 465
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    Published online on: July 30, 2024
       https://doi.org/10.3892/ol.2024.14598
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Abstract

Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 (MALT1) inhibitors are effective in attenuating the progression of several types of cancer. However, their role in lung cancer requires further investigation. Therefore, the present study aimed to explore the effect of the MALT1 inhibitor, MI‑2, on the behavior of non‑small cell lung cancer (NSCLC) cells and to uncover their possible underlying mechanism of action. The mRNA and protein expression levels of MALT1 were detected in the human normal lung epithelial cell line BEAS‑2B, and the NSCLC cell lines, NCI‑H1299, NCI‑H1650, HCC827, A549 and NCI‑H23. Subsequently, NCI‑H1650 and A549 cells were treated with MI‑2. Additionally, NCI‑H1650 and A549 cells were co‑treated with anisomycin, a c‑JUN N‑terminal kinase (JNK) pathway activator, with or without MI‑2. The results illustrated that the mRNA and protein expression levels of MALT1 were significantly increased in NCI‑H1299, NCI‑H1650, A549 and NCI‑H23 cells compared with those in BEAS‑2B cells. Treatment of NCI‑H1650 and A549 cells with MI‑2 for 72 h reduced the optical density value as determined using the Cell Counting Kit‑8 assay. Consistently, the 5‑ethynyl‑2'‑deoxyuridine assay also showed that proliferation was reduced in MI‑2‑treated NSCLC cells. In addition, MI‑2 downregulated B‑cell lymphoma 2 (BCL2), and enhanced BCL2‑associated X‑protein expression and apoptotic rate in NCI‑H1650 and A549 cells. These findings indicated that MI‑2 could inhibit NCI‑H1650 and A549 cell proliferation and promote apoptosis. Furthermore, treatment of cells with MI‑2 only attenuated the migration and invasion of NCI‑H1650 cells. Notably, MI‑2 decreased the expression levels of phosphorylated (p)‑JNK and p‑c‑JUN in NCI‑H1650 and A549 cells, thus suggesting that MI‑2 could suppress the JNK/c‑JUN signaling pathway. However, NSCLC cell co‑treatment with anisomycin (JNK pathway activator) reversed the effect of MI‑2 on the proliferation, apoptosis and activation of the JNK/c‑JUN pathway in NCI‑H1650 and A549 cells. In conclusion, the present study demonstrated that the MALT1 inhibitor, MI‑2, could suppress NSCLC cell proliferation, migration and invasion, and induce apoptosis via inactivating the JNK/c‑JUN pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wu C, Ge W and Wu Y: Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway. Oncol Lett 28: 465, 2024.
APA
Wu, C., Ge, W., & Wu, Y. (2024). Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway. Oncology Letters, 28, 465. https://doi.org/10.3892/ol.2024.14598
MLA
Wu, C., Ge, W., Wu, Y."Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway". Oncology Letters 28.4 (2024): 465.
Chicago
Wu, C., Ge, W., Wu, Y."Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway". Oncology Letters 28, no. 4 (2024): 465. https://doi.org/10.3892/ol.2024.14598
Copy and paste a formatted citation
x
Spandidos Publications style
Wu C, Ge W and Wu Y: Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway. Oncol Lett 28: 465, 2024.
APA
Wu, C., Ge, W., & Wu, Y. (2024). Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway. Oncology Letters, 28, 465. https://doi.org/10.3892/ol.2024.14598
MLA
Wu, C., Ge, W., Wu, Y."Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway". Oncology Letters 28.4 (2024): 465.
Chicago
Wu, C., Ge, W., Wu, Y."Mucosa‑associated lymphoid tissue lymphoma translocation protein 1 inhibitor, MI‑2, attenuates non‑small cell lung cancer cell proliferation, migration and invasion, and promotes apoptosis by suppressing the JNK/c‑JUN pathway". Oncology Letters 28, no. 4 (2024): 465. https://doi.org/10.3892/ol.2024.14598
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