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Article

Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine

  • Authors:
    • Nannan Liu
    • Shanshan Liu
    • Xueshuang Zhang
    • Wenzhu Tian
    • Heqiang Jia
    • Xin Ye
    • Xiaoyu Yan
    • Chunyan Yu
    • Huimei Yu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, College of Basic Medicine, Beihua University, Jilin, Jilin 132013, P.R. China, Department of Transplantation, Hebei Yanda Ludaopei Hospital, Langfang, Hebei 065200, P.R. China, Department of Hematology, Changchun Central Hospital, Changchun, Jilin 130041, P.R. China, Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China, Key Laboratory of Pathobiology, Department of Pathophysiology, Ministry of Education, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China
  • Article Number: 529
    |
    Published online on: September 3, 2024
       https://doi.org/10.3892/ol.2024.14662
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Abstract

Cisplatin resistance in ovarian cancer cells is mainly apoptosis resistant. Although other types of programmed cell death are highly involved in chemoresistance, which type can overcome cisplatin resistance remains unclear. The present study observed that cisplatin‑sensitive SKOV3 cells and cisplatin‑resistant SKOV3/DDP cells had different levels of sensitivity to sulfasalazine (SAS). The present study aimed to investigate the effect of SAS on necroptosis under the same inhibition rate in these two types of cells. Necroptosis inhibitor Necrostatin‑1 (Nec‑1) attenuated SAS‑induced SKOV3/DDP cytotoxicity. SAS decreased SKOV3/DDP cells survival rate, accompanied by decreased cell adhesion and spreading. SAS treatment activated necrosome formation in SKOV3/DDP cells, suggesting the possibility of necroptosis. p62/sequestosome‑1 (SQSTM1) protein expression levels were also increased over the same time period. The transfection of small interfering (si)‑p62 could decrease the ratios of phosphorylated (p)‑receptor‑interacting serine/threonine kinase 1 (RIP1)/RIP1, p‑receptor‑interacting serine/threonine kinase 3 (RIP3)/RIP3 and p‑mixed lineage kinase domain‑like protein (MLKL)/MLKL proteins in SKOV3/DDP cells. Under the si‑p62 condition, there was no increase in the rate of cell survival in Nec‑1 and SAS combination group compared with SAS. The zinc finger domain deletion of p62/SQSTM1 effectively decreased the expression levels of necroptosis‑related p‑proteins. Collectively, certain drugs were able to induce necroptosis in SKOV3/DDP, while p62/RIP1/RIP3/MLKL was associated with the induction of necroptosis and with increasing the sensitivity of cisplatin‑resistant ovarian cancer cells, which provided evidence for potential as a therapeutic target for overcoming resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Liu N, Liu S, Zhang X, Tian W, Jia H, Ye X, Yan X, Yu C and Yu H: Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine. Oncol Lett 28: 529, 2024.
APA
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X. ... Yu, H. (2024). Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine. Oncology Letters, 28, 529. https://doi.org/10.3892/ol.2024.14662
MLA
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X., Yan, X., Yu, C., Yu, H."Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine". Oncology Letters 28.5 (2024): 529.
Chicago
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X., Yan, X., Yu, C., Yu, H."Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine". Oncology Letters 28, no. 5 (2024): 529. https://doi.org/10.3892/ol.2024.14662
Copy and paste a formatted citation
x
Spandidos Publications style
Liu N, Liu S, Zhang X, Tian W, Jia H, Ye X, Yan X, Yu C and Yu H: Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine. Oncol Lett 28: 529, 2024.
APA
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X. ... Yu, H. (2024). Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine. Oncology Letters, 28, 529. https://doi.org/10.3892/ol.2024.14662
MLA
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X., Yan, X., Yu, C., Yu, H."Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine". Oncology Letters 28.5 (2024): 529.
Chicago
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H., Ye, X., Yan, X., Yu, C., Yu, H."Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine". Oncology Letters 28, no. 5 (2024): 529. https://doi.org/10.3892/ol.2024.14662
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