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Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability

  • Authors:
    • Bernardo Xavier Tolentino‑Molina
    • Jaqueline Loaeza‑Loaeza
    • Arturo Ortega‑Soto
    • Yaneth Castro‑Coronel
    • Gloria Fernández‑Tilapa
    • Daniel Hernández‑Sotelo
  • View Affiliations

    Affiliations: Laboratory of Cancer Epigenetics, School of Chemical and Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero 39070, Mexico, Laboratory of Neurotoxicology, Department of Toxicology, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City 07300, Mexico, Laboratory of Cytopathology and Histochemistry, School of Chemical and Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero 39070, Mexico, Clinical Research Laboratory, School of Chemical and Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero 39070, Mexico
  • Published online on: November 26, 2024     https://doi.org/10.3892/ol.2024.14820
  • Article Number: 74
  • Copyright: © Tolentino‑Molina et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer (CC) is considered a public health problem. Circular RNAs (circRNAs) serve important roles in different types of cancer, including CC. However, the mechanisms used by circRNAs to facilitate CC progression are currently unclear. The present study analyzed the effects of hsa_circ_0009910 knockdown on microRNA (miRNA/miR)‑198 and mesenchymal‑epithelial transition factor (c‑Met) expression levels and its impact on apoptosis and the viability of HeLa cells. Differentially expressed circRNAs in CC were identified using analysis of circRNA microarray data. Bioinformatics analysis was performed to predict circRNA‑microRNA (miRNA) and miRNA‑mRNA interactions. The knockdown of hsa_circ_0009910 in HeLa cells was performed using small interfering RNA and the expression levels of hsa_circ_0009910, miR‑198 and c‑Met were assessed using reverse transcription‑quantitative PCR. The viability and apoptosis of HeLa cells were evaluated using MTT, neutral red uptake and ApoLive‑Glo™ multiplex assays. Hsa_circ_0009910 was significantly upregulated in HeLa cells and the knockdown of hsa_circ_0009910 increased miRNA‑198 expression levels, reduced c‑Met expression levels and decreased cellular viability, but not apoptosis, in HeLa cells. Overall, these results indicated that hsa_circ_0009910 could act as a molecular sponge of miRNA‑198 and contribute to the upregulation of c‑Met expression levels. The hsa_circ_0009910/miRNA‑198/c‑Met interaction network affects the viability, but not apoptosis, of HeLa cells. Based on this mechanism, the present study suggests that hsa_circ_0009910 may be a promising biomarker for CC.
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Copy and paste a formatted citation
Spandidos Publications style
Tolentino‑Molina BX, Loaeza‑Loaeza J, Ortega‑Soto A, Castro‑Coronel Y, Fernández‑Tilapa G and Hernández‑Sotelo D: Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability. Oncol Lett 29: 74, 2025.
APA
Tolentino‑Molina, B.X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., & Hernández‑Sotelo, D. (2025). Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability. Oncology Letters, 29, 74. https://doi.org/10.3892/ol.2024.14820
MLA
Tolentino‑Molina, B. X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., Hernández‑Sotelo, D."Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability". Oncology Letters 29.2 (2025): 74.
Chicago
Tolentino‑Molina, B. X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., Hernández‑Sotelo, D."Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability". Oncology Letters 29, no. 2 (2025): 74. https://doi.org/10.3892/ol.2024.14820
Copy and paste a formatted citation
x
Spandidos Publications style
Tolentino‑Molina BX, Loaeza‑Loaeza J, Ortega‑Soto A, Castro‑Coronel Y, Fernández‑Tilapa G and Hernández‑Sotelo D: Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability. Oncol Lett 29: 74, 2025.
APA
Tolentino‑Molina, B.X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., & Hernández‑Sotelo, D. (2025). Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability. Oncology Letters, 29, 74. https://doi.org/10.3892/ol.2024.14820
MLA
Tolentino‑Molina, B. X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., Hernández‑Sotelo, D."Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability". Oncology Letters 29.2 (2025): 74.
Chicago
Tolentino‑Molina, B. X., Loaeza‑Loaeza, J., Ortega‑Soto, A., Castro‑Coronel, Y., Fernández‑Tilapa, G., Hernández‑Sotelo, D."Hsa_circ_0009910 knockdown in HeLa cells increases miR‑198 expression levels and decreases c‑Met expression levels and cell viability". Oncology Letters 29, no. 2 (2025): 74. https://doi.org/10.3892/ol.2024.14820
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