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Article Open Access

miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1

  • Authors:
    • Ying Jin
    • Zhijun Wang
    • Yuanshan Liang
    • Yiting Jiang
    • Fayang Yuan
    • Tian Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 96
    |
    Published online on: December 10, 2024
       https://doi.org/10.3892/ol.2024.14842
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Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor with a high incidence rate in certain regions. MicroRNA (miRNA/miR)‑22‑3p is implicated in the regulation of tumorigenesis and progression. However, the biological role of miRNA‑22‑3p in the progression of NPC remains unclear. The present study aimed to assess the effects of miRNA‑22‑3p overexpression on the cell viability and migration of NPC cells. The cell viability and migration of HK‑1 cells was evaluated using Transwell, wound healing and Cell Counting Kit‑8 assays. To assess the epithelial‑mesenchymal transition ability of NPC cells, the expression of E‑cadherin, vimentin and N‑cadherin was evaluated using western blot analysis. The results revealed expression of miRNA‑22‑3p was significantly decreased in NPC tissues compared with para‑cancerous tissues. Decreased expression of miRNA‑22‑3p was also observed in NPC cell lines (C666‑1 and HK‑1). The overexpression of miRNA‑22‑3p reduced HK‑1 cell viability and migration. In addition, a dual luciferase reporter assay revealed that miRNA‑22‑3p functioned as a molecular sponge for forkhead box protein 1 (FOXP1). Notably, FOXP1 overexpression counteracted the suppressive effects induced by transfection with miRNA‑22‑3p mimic on HK‑1 cell viability and migration. Therefore, these data indicate that miRNA‑22‑3p may be a clinically valuable biomarker for the therapy of NPC.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Y, Wang Z, Liang Y, Jiang Y, Yuan F and Zhang T: miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1. Oncol Lett 29: 96, 2025.
APA
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., & Zhang, T. (2025). miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1. Oncology Letters, 29, 96. https://doi.org/10.3892/ol.2024.14842
MLA
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., Zhang, T."miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1". Oncology Letters 29.2 (2025): 96.
Chicago
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., Zhang, T."miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1". Oncology Letters 29, no. 2 (2025): 96. https://doi.org/10.3892/ol.2024.14842
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y, Wang Z, Liang Y, Jiang Y, Yuan F and Zhang T: miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1. Oncol Lett 29: 96, 2025.
APA
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., & Zhang, T. (2025). miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1. Oncology Letters, 29, 96. https://doi.org/10.3892/ol.2024.14842
MLA
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., Zhang, T."miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1". Oncology Letters 29.2 (2025): 96.
Chicago
Jin, Y., Wang, Z., Liang, Y., Jiang, Y., Yuan, F., Zhang, T."miRNA‑22‑3p inhibits cell viability and metastasis of nasopharyngeal carcinoma by targeting FOXP1". Oncology Letters 29, no. 2 (2025): 96. https://doi.org/10.3892/ol.2024.14842
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