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Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and in vitro insights

  • Authors:
    • Shazia Fathima Jaffer Hussain
    • Shahabe Saquib Abullais
    • Kavitha Bottu
    • Logalakshmi Thirumani
    • Iffath Misbah
    • Inamul Hasan Madar
    • Nuha S. Alghamdi
    • Mohmed Isaqali Karobari
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu 600077, India, Department of Periodontics and Community Dental Sciences, College of Dentistry, King Khalid University, Abha, Aseer 62529, Kingdom of Saudi Arabia, Department of Oral Pathology and Microbiology, Meenakshi Ammal Dental College and Hospitals, Meenakshi Academy of Higher Education and Research, Chennai, Tamil Nadu 600095, India, Center for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical And Technical Sciences, Chennai, Tamil Nadu 602105, India, Department of Radio‑diagnosis, Saveetha Medical College, Saveetha Institute of Medical and Technical Science, Chennai, Tamil Nadu 602105, India, Department of Restorative Dental Sciences, College of Dentistry, King Khalid University, Abha, Aseer 62529, Kingdom of Saudi Arabia, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu 600077, India
    Copyright: © Hussain et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 115
    |
    Published online on: January 3, 2025
       https://doi.org/10.3892/ol.2025.14862
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Abstract

The present study investigated the involvement of human papillomavirus (HPV)16 and HPV18 in oropharyngeal malignancies in order to understand the oncogenic mechanisms, and to identify biomarkers for early detection and treatment targets. Given the rising incidence of HPV‑associated cancer, particularly in India, this holds significance in elucidating the molecular basis of these diseases. Structural validation of HPV16 and 18 oncoproteins E6 and E7 was conducted using computational tools, while gene expression profiles related to oral squamous cell carcinoma (OSCC) were analyzed to assess differential expression. The presence of HPV in patient tissue sections was examined using reverse transcription‑PCR. The present study revealed the interactions of HPV16 and 18 E6/E7 oncoproteins, highlighting their role in cancer progression by targeting key tumor suppressors, such as p53 and retinoblastoma protein. Further analysis demonstrated the involvement of HPV16 and 18 E6/E7 oncoproteins in cancer pathways, signaling and telomere regulation, which supports the development of future targeted therapies. HPV16 and 18 E6/E7 represent promising therapeutic targets in OSCC, and provide further insights into potential diagnostic and treatment avenues. The present study contributes to the current understanding of HPV‑associated cancer and innovative strategies in disease management.
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Copy and paste a formatted citation
Spandidos Publications style
Hussain SJ, Abullais SS, Bottu K, Thirumani L, Misbah I, Madar IH, Alghamdi NS and Karobari MI: Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncol Lett 29: 115, 2025.
APA
Hussain, S.J., Abullais, S.S., Bottu, K., Thirumani, L., Misbah, I., Madar, I.H. ... Karobari, M.I. (2025). Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncology Letters, 29, 115. https://doi.org/10.3892/ol.2025.14862
MLA
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29.3 (2025): 115.
Chicago
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29, no. 3 (2025): 115. https://doi.org/10.3892/ol.2025.14862
Copy and paste a formatted citation
x
Spandidos Publications style
Hussain SJ, Abullais SS, Bottu K, Thirumani L, Misbah I, Madar IH, Alghamdi NS and Karobari MI: Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncol Lett 29: 115, 2025.
APA
Hussain, S.J., Abullais, S.S., Bottu, K., Thirumani, L., Misbah, I., Madar, I.H. ... Karobari, M.I. (2025). Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights. Oncology Letters, 29, 115. https://doi.org/10.3892/ol.2025.14862
MLA
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29.3 (2025): 115.
Chicago
Hussain, S. J., Abullais, S. S., Bottu, K., Thirumani, L., Misbah, I., Madar, I. H., Alghamdi, N. S., Karobari, M. I."Molecular analysis of HPV16 and HPV18 oncogenes in oral squamous cell carcinoma: Structural, transcriptomic and <em>in vitro</em> insights". Oncology Letters 29, no. 3 (2025): 115. https://doi.org/10.3892/ol.2025.14862
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