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Article Open Access

Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis

  • Authors:
    • Ni Zhang
    • Qingbin Liu
    • Qian Wang
    • Xiuli Liu
    • Suya Zhang
    • Xinchen Tian
    • Long Li
    • Shuanglong Wang
    • Bin Lv
    • Shulong Jiang
  • View Affiliations / Copyright

    Affiliations: Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250000, P.R. China, Clinical Medical Laboratory Center, Jining First People's Hospital, Shandong First Medical University, Jining, Shandong 272000, P.R. China, Department of Ultrasonic Medicine, Jining First People's Hospital, Shandong First Medical University, Jining, Shandong 272000, P.R. China, Department of Breast and Thyroid Surgery, Jining First People's Hospital, Shandong First Medical University, Jining, Shandong 272000, P.R. China, Department of Ultrasonic Medicine, Jining First People's Hospital, Shandong First Medical University, Jining, Shandong 272000, P.R. China, Clinical Medical Laboratory Center, Jining First People's Hospital, Shandong First Medical University, Jining, Shandong 272000, P.R. China, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 130
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    Published online on: January 7, 2025
       https://doi.org/10.3892/ol.2025.14876
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Abstract

Papillary thyroid cancer (PTC) is frequently associated with inflammation and lymph node metastasis. Single‑cell RNA sequencing (scRNA‑seq) is a powerful tool to uncover rare cellular subpopulations and investigate the diverse functions inside tissue microenvironments. In the present study, scRNA‑seq analysis was employed to analyze the differences in macrophages, dendritic cells (DCs) and T cells between a metastatic PTC (PTC‑M) and its adjacent normal tissues, as well as a PTC tumor without metastasis. The findings revealed significant heterogeneity in immune cell populations in PTC‑M, suggesting that immunosuppressive components contribute to the development and metastasis of PTC. The current study revealed that the presence of alternatively activated M2 macrophages, conventional type 2 DCs (DC2s) and regulatory T cells (Tregs) was associated with increased lymph node metastasis and a more advanced stage of cancer. On the other hand, monocytes and B cells may have a beneficial effect in fighting against tumors. A group of tumor‑associated DC2s expressing both LAMP3 and CCL22 were shown to have a variety of immune‑related ligands. These cells have the ability to attract CD4+ T cells through communication between cells in the microenvironment. In this study, the immunological composition was examined at the level of individual cells and new prospective treatment approaches for PTC‑M were identified. The results support the hypothesis that myeloid cells and Tregs significantly contribute to tumor progression and metastasis by shaping the tumor microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang N, Liu Q, Wang Q, Liu X, Zhang S, Tian X, Li L, Wang S, Lv B, Jiang S, Jiang S, et al: Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis. Oncol Lett 29: 130, 2025.
APA
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X. ... Jiang, S. (2025). Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis. Oncology Letters, 29, 130. https://doi.org/10.3892/ol.2025.14876
MLA
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X., Li, L., Wang, S., Lv, B., Jiang, S."Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis". Oncology Letters 29.3 (2025): 130.
Chicago
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X., Li, L., Wang, S., Lv, B., Jiang, S."Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis". Oncology Letters 29, no. 3 (2025): 130. https://doi.org/10.3892/ol.2025.14876
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang N, Liu Q, Wang Q, Liu X, Zhang S, Tian X, Li L, Wang S, Lv B, Jiang S, Jiang S, et al: Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis. Oncol Lett 29: 130, 2025.
APA
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X. ... Jiang, S. (2025). Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis. Oncology Letters, 29, 130. https://doi.org/10.3892/ol.2025.14876
MLA
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X., Li, L., Wang, S., Lv, B., Jiang, S."Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis". Oncology Letters 29.3 (2025): 130.
Chicago
Zhang, N., Liu, Q., Wang, Q., Liu, X., Zhang, S., Tian, X., Li, L., Wang, S., Lv, B., Jiang, S."Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasis". Oncology Letters 29, no. 3 (2025): 130. https://doi.org/10.3892/ol.2025.14876
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