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RNA analysis of patients with benign and malignant pulmonary nodules

  • Authors:
    • Guangjie Liu
    • Qingyi Liu
    • Yutong He
    • Lai Wei
    • Di Liang
    • Shaonan Xie
    • Ning Zhang
    • Nan Geng
    • Liwen Zhang
    • Yajie Huang
    • Fang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China, Department of Cancer Institute, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China, Department of Computed Tomography and Magnetic Resonance Imaging, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China, Department of Respiratory Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China, Hebei Key Laboratory of Environment and Human Health, Department of Epidemiology and Statistics, School of Public Health, Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China, Department of Hospital Quality and Control, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050001, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 132
    |
    Published online on: January 7, 2025
       https://doi.org/10.3892/ol.2025.14878
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Abstract

Pulmonary nodules are the main manifestations of early lung cancer. Non‑small cell lung cancer is the most common histological type of lung cancer, and the main histological classification of non‑small cell lung cancer is lung adenocarcinoma. The present study aimed to analyze the differentially expressed genes between patients with benign and malignant pulmonary nodules, and to identify potential therapeutic targets for lung adenocarcinoma. Sequencing data for benign and malignant pulmonary nodule samples and samples with no nodules were obtained from the National Center for Biotechnology Information Gene Expression Omnibus GSE135304 dataset. Differential gene analysis showed that S100 calcium binding protein P (S100P), ribonuclease A family member 2 (RNASE2), cytochrome c oxidase subunit 7C and mast cell expressed membrane protein 1 (C19orf59) were significantly upregulated among the blood samples collected from patients with malignant pulmonary nodules. Results from Kaplan‑Meier plotter datasets showed that S100P, RNASE2 and C19orf59 were associated with the prognosis of lung cancer. RNASE2 expression was positively associated with nodule size and negatively associated with lung cancer prognosis. Moreover, RNASE2 was highly expressed in lung adenocarcinoma tissues compared with that in normal tissues. CCK‑8 and Transwell assays indicated that overexpressed RNASE2 promoted the proliferation, migration and invasion of lung adenocarcinoma cells. In lung adenocarcinoma cells, RNASE2 was identified as a downstream target of microRNA (miR)‑185‑5p and was regulated by it. Inhibited cell proliferation, migration and invasion were observed following overexpression of miR‑185‑5p. Overexpression of RNASE2 reversed the inhibitory effect of miR‑185‑5p overexpression. In conclusion, in blood samples from patients with malignant pulmonary nodules and lung adenocarcinoma tissues, RNASE2 was found to be upregulated. High RNASE2 expression was associated with poor overall survival. miR‑185‑5p inhibited the proliferation, migration and invasion of lung adenocarcinoma cells by downregulating RNASE2 expression. These findings have implications for guiding therapeutic strategies.
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View References

1 

Sharma P, Mahadevia H, Donepudi S, Kujtan L, Gustafson B, Ponvilawan B, Al-Obaidi A, Subramanian J and Bansal D: A novel EGFR germline mutation in lung adenocarcinoma: Case report and literature review. Clin Lung Cancer. 25:479–482. 2024. View Article : Google Scholar : PubMed/NCBI

2 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 71:209–249. 2021. View Article : Google Scholar : PubMed/NCBI

3 

Sun R, Hou Z, Zhang Y and Jiang B: Drug resistance mechanisms and progress in the treatment of EGFR-mutated lung adenocarcinoma. Oncol Lett. 24:4082022. View Article : Google Scholar : PubMed/NCBI

4 

Li N and Zhan X: Identification of pathology-specific regulators of m(6)A RNA modification to optimize lung cancer management in the context of predictive, preventive, and personalized medicine. EPMA J. 11:485–504. 2020. View Article : Google Scholar : PubMed/NCBI

5 

Shu J, Jiang J and Zhao G: Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis. Front Immunol. 14:11778472023. View Article : Google Scholar : PubMed/NCBI

6 

Liu A, Wang Z, Yang Y, Wang J, Dai X, Wang L, Lu Y and Xue F: Preoperative diagnosis of malignant pulmonary nodules in lung cancer screening with a radiomics nomogram. Cancer Commun (Lond). 40:16–24. 2020. View Article : Google Scholar : PubMed/NCBI

7 

Prosper AE, Kammer MN, Maldonado F, Aberle DR and Hsu W: Expanding role of advanced image analysis in CT-detected indeterminate pulmonary nodules and early lung cancer characterization. Radiology. 309:e2229042023. View Article : Google Scholar : PubMed/NCBI

8 

Ma L, Qiu B, Zhang J, Li QW, Wang B, Zhang XH, Qiang MY, Chen ZL, Guo SP and Liu H: Survival and prognostic factors of non-small cell lung cancer patients with postoperative locoregional recurrence treated with radical radiotherapy. Chin J Cancer. 36:932017. View Article : Google Scholar : PubMed/NCBI

9 

Hirsch FR, Scagliotti GV, Mulshine JL, Kwon R, Curran Jr WJ, Wu YL and Paz-Ares L: Lung cancer: Current therapies and new targeted treatments. Lancet. 389:299–311. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Hajipour S, Hosseini SM, Irani S and Tavallaie M: Identification of novel potential drugs and miRNAs biomarkers in lung cancer based on gene co-expression network analysis. Genomics Inform. 21:e382023. View Article : Google Scholar : PubMed/NCBI

11 

Ma H, Jiang S, Yuan Y, Li J, Li Y, Lv Y, Du T, Guan J, Jiang X, Tian L, et al: RUNX1 promotes proliferation and migration in non-small cell lung cancer cell lines via the mTOR pathway. FASEB J. 37:e231952023. View Article : Google Scholar : PubMed/NCBI

12 

Jiang J, Zhang Y, Wang J, Yang X, Ren X, Huang H, Wang J, Lu J, Zhong Y, Lin Z, et al: Identification of CDT1 as a prognostic marker in human lung adenocarcinoma using bioinformatics approaches. Peer J. 11:e161662023. View Article : Google Scholar : PubMed/NCBI

13 

Gong L, Zhang D, Dong Y, Lei Y, Qian Y, Tan X, Han S and Wang J: Integrated bioinformatics analysis for identificating the therapeutic targets of aspirin in small cell lung cancer. J Biomed Inform. 88:20–28. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Zhang H, Peng Y and Guo Y: Pulmonary nodules detection based on multi-scale attention networks. Sci Rep. 12:14662022. View Article : Google Scholar : PubMed/NCBI

15 

Kossenkov AV, Qureshi R, Dawany NB, Wickramasinghe J, Liu Q, Majumdar RS, Chang C, Widura S, Kumar T, Horng WH, et al: A gene expression classifier from whole blood distinguishes benign from malignant lung nodules detected by low-dose CT. Cancer Res. 79:263–273. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Wei L, Wu Y, Cai S, Qin Y, Xing S and Wang Z: Long non-coding RNA linc01224 regulates hypopharyngeal squamous cell carcinoma growth through interactions with miR-485-5p and IGF2BP3. J Cancer. 14:3009–3022. 2023. View Article : Google Scholar : PubMed/NCBI

17 

Song S, Xie S, Liu X, Li S, Wang L, Jiang X and Lu D: miR-3200 accelerates the growth of liver cancer cells by enhancing Rab7A. Noncoding RNA Res. 8:675–685. 2023. View Article : Google Scholar : PubMed/NCBI

18 

Lai C, He N, Zeng J, Long C, Shi M, Li J, Ma S, Xiong Y and Liang X: Highly expressed miR-144-3p promotes the proliferation, migration and invasion of colon carcinoma cells by activating the Wnt/beta-catenin signaling pathway through targeting SFRP1. J Cancer. 14:3117–3129. 2023. View Article : Google Scholar : PubMed/NCBI

19 

Wang D, Zhang S, Zhao M and Chen F: LncRNA MALAT1 accelerates non-small cell lung cancer progression via regulating miR-185-5p/MDM4 axis. Cancer Med. 9:9138–9149. 2020. View Article : Google Scholar : PubMed/NCBI

20 

Li Y, Hu Y, Wu Y, Zhang D and Huang D: LINC00205 promotes tumor malignancy of lung adenocarcinoma through sponging miR-185-5p. Lab Med. 53:39–46. 2022. View Article : Google Scholar : PubMed/NCBI

21 

Yu N, Gong H, Chen W and Peng W: CircRNA ZKSCAN1 promotes lung adenocarcinoma progression by miR-185-5p/TAGLN2 axis. Thorac Cancer. 14:1467–1476. 2023. View Article : Google Scholar : PubMed/NCBI

22 

Rehbein G, Simm A, Hofmann HS, Silber RE and Bartling B: Molecular regulation of S100P in human lung adenocarcinomas. Int J Mol Med. 22:69–77. 2008.PubMed/NCBI

23 

Tan BS, Yang MC, Singh S, Chou YC, Chen HY, Wang MY, Wang YC and Chen RH: LncRNA NORAD is repressed by the YAP pathway and suppresses lung and breast cancer metastasis by sequestering S100P. Oncogene. 38:5612–5626. 2019. View Article : Google Scholar : PubMed/NCBI

24 

Hsu YL, Hung JY, Liang YY, Lin YS, Tsai MJ, Chou SH, Lu CY and Kuo PL: S100P interacts with integrin alpha7 and increases cancer cell migration and invasion in lung cancer. Oncotarget. 6:29585–29598. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Chien MH, Lee WJ, Hsieh FK, Li CF, Cheng TY, Wang MY, Chen JS, Chow JM, Jan YH, Hsiao M, et al: Keap1-Nrf2 interaction suppresses cell motility in lung adenocarcinomas by targeting the s100p protein. Clin Cancer Res. 21:4719–4732. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Wu T, Chen Y, Yang L, Wang X, Chen K and Xu D: Ribonuclease a family member 2 promotes the malignant progression of glioma through the PI3K/Akt signaling pathway. Front Oncol. 12:9210832022. View Article : Google Scholar : PubMed/NCBI

27 

Ma J, Bai Y, Chen F, Zhou F, Zhang L, Xue P and Wang D: MicroRNA-185-5p targets tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta to regulate non-small cell lung cancer progression. J Cardiothorac Surg. 18:2412023. View Article : Google Scholar : PubMed/NCBI

28 

Wen H, Liu Z, Tang J and Bu L: MiR-185-5p targets RAB35 gene to regulate tumor cell-derived exosomes-mediated proliferation, migration and invasion of non-small cell lung cancer cells. Aging (Albany NY). 13:21435–21450. 2021. View Article : Google Scholar : PubMed/NCBI

29 

Li H, Han D, Hou Y, Chen H and Chen Z: Statistical inference methods for two crossing survival curves: A comparison of methods. PLoS One. 10:e01167742015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu G, Liu Q, He Y, Wei L, Liang D, Xie S, Zhang N, Geng N, Zhang L, Huang Y, Huang Y, et al: RNA analysis of patients with benign and malignant pulmonary nodules. Oncol Lett 29: 132, 2025.
APA
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S. ... Liu, F. (2025). RNA analysis of patients with benign and malignant pulmonary nodules. Oncology Letters, 29, 132. https://doi.org/10.3892/ol.2025.14878
MLA
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S., Zhang, N., Geng, N., Zhang, L., Huang, Y., Liu, F."RNA analysis of patients with benign and malignant pulmonary nodules". Oncology Letters 29.3 (2025): 132.
Chicago
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S., Zhang, N., Geng, N., Zhang, L., Huang, Y., Liu, F."RNA analysis of patients with benign and malignant pulmonary nodules". Oncology Letters 29, no. 3 (2025): 132. https://doi.org/10.3892/ol.2025.14878
Copy and paste a formatted citation
x
Spandidos Publications style
Liu G, Liu Q, He Y, Wei L, Liang D, Xie S, Zhang N, Geng N, Zhang L, Huang Y, Huang Y, et al: RNA analysis of patients with benign and malignant pulmonary nodules. Oncol Lett 29: 132, 2025.
APA
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S. ... Liu, F. (2025). RNA analysis of patients with benign and malignant pulmonary nodules. Oncology Letters, 29, 132. https://doi.org/10.3892/ol.2025.14878
MLA
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S., Zhang, N., Geng, N., Zhang, L., Huang, Y., Liu, F."RNA analysis of patients with benign and malignant pulmonary nodules". Oncology Letters 29.3 (2025): 132.
Chicago
Liu, G., Liu, Q., He, Y., Wei, L., Liang, D., Xie, S., Zhang, N., Geng, N., Zhang, L., Huang, Y., Liu, F."RNA analysis of patients with benign and malignant pulmonary nodules". Oncology Letters 29, no. 3 (2025): 132. https://doi.org/10.3892/ol.2025.14878
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