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MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression

  • Authors:
    • Xiumei Zhang
    • Lin Cong
    • Rong Yu
    • Qianwen Yu
    • Xian Hou
    • Yonghua Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The People's Hospital of Xinghua City, Xinghua, Jiangsu 225700, P.R. China, Department of Gastrointestinal Surgery, The People's Hospital of Xinghua City, Xinghua, Jiangsu 225700, P.R. China, Department of Radiology, The People's Hospital of Xinghua City, Xinghua, Jiangsu 225700, P.R. China, Department of Breast Surgery, The People's Hospital of Xinghua City, Xinghua, Jiangsu 225700, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 151
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    Published online on: January 22, 2025
       https://doi.org/10.3892/ol.2025.14897
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Abstract

The present study aimed to investigate the effects of microRNA (miR)‑96 on the proliferation and migration of breast cancer cells, and indicated that miR‑96 may have a promoting role in breast cancer by inhibiting Smad7. Reverse transcription‑quantitative (RT‑q)PCR was used to detect the expression levels of miR‑96 and Smad7 in breast cancer tissues and adjacent tissues. Western blotting and immunohistochemistry were conducted to determine the expression levels of SMAD7 in breast cancer and adjacent tissues. A dual luciferase assay was performed to verify the targeted binding between miR‑96 and Smad7. Furthermore, the different expression patterns of miR‑96 and Smad7 were compared in various breast cancer cell lines using RT‑qPCR and western blotting. Among these cell lines, MDA‑MB‑231, which exhibited the highest expression of miR‑96, was chosen for subsequent functional verification. The expression levels of miR‑96 were significantly higher in breast cancer tissues compared with those in adjacent tissues. By contrast, the expression levels of Smad7 were significantly lower in breast cancer tissues compared with those in adjacent tissues. The dual luciferase assay revealed a targeted binding effect between miR‑96 and Smad7. Notably, transfection with miR‑96‑5p mimics and short hairpin RNA‑Smad7 markedly promoted the proliferation, adhesion, invasion and migration of breast cancer cells. Conversely, transfection with a miR‑96‑5p inhibitor and Smad7 overexpression plasmid exhibited the opposite trend. In conclusion, the expression levels of miR‑96 were significantly elevated in breast cancer tissues compared with those in adjacent tissues. Overexpression of miR‑96 was shown to promote the migration of breast cancer cells by downregulating the expression of Smad7. These findings indicated that miR‑96 may serve as a prognostic marker for breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Cong L, Yu R, Yu Q, Hou X and Zhou Y: MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression. Oncol Lett 29: 151, 2025.
APA
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., & Zhou, Y. (2025). MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression. Oncology Letters, 29, 151. https://doi.org/10.3892/ol.2025.14897
MLA
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., Zhou, Y."MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression". Oncology Letters 29.3 (2025): 151.
Chicago
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., Zhou, Y."MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression". Oncology Letters 29, no. 3 (2025): 151. https://doi.org/10.3892/ol.2025.14897
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Cong L, Yu R, Yu Q, Hou X and Zhou Y: MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression. Oncol Lett 29: 151, 2025.
APA
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., & Zhou, Y. (2025). MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression. Oncology Letters, 29, 151. https://doi.org/10.3892/ol.2025.14897
MLA
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., Zhou, Y."MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression". Oncology Letters 29.3 (2025): 151.
Chicago
Zhang, X., Cong, L., Yu, R., Yu, Q., Hou, X., Zhou, Y."MicroRNA‑96 promotes the proliferation and migration of breast cancer cells by inhibiting Smad7 expression". Oncology Letters 29, no. 3 (2025): 151. https://doi.org/10.3892/ol.2025.14897
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