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Article Open Access

Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation

  • Authors:
    • Runyang Yin
    • Jing Gao
    • Yang Liu
    • Chunyan Guo
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    Affiliations: Department of Anesthesiology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China, First Clinical Medical College, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China, Department of Clinical Laboratory, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 194
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    Published online on: February 21, 2025
       https://doi.org/10.3892/ol.2025.14940
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Abstract

Although 70% of patients with estrogen receptor‑positive breast cancer benefit from tamoxifen (TAM) therapy, the development of resistance to TAM leads to high rates of metastasis and a poor prognosis. Propofol, a commonly used anesthetic, can inhibit the occurrence and progression of breast cancer. In the present study, the effects of propofol on TAM‑resistant (TR) breast cancer cells were evaluated. MCF7‑TR cells were treated with or without propofol. Subsequently, cell cycle progression and the induction of apoptosis were detected by flow cytometry, whereas cell proliferation was assessed using Cell Counting Kit‑8 and colony formation assays. Furthermore, the potential transcriptional regulatory effects of propofol on MCF7‑TR cells were investigated using RNA sequencing. The results indicated that propofol significantly promoted cell cycle arrest, induced apoptosis, and inhibited proliferation and colony formation in MCF7‑TR cells. Furthermore, transcriptome sequencing analysis revealed 1,065 differentially expressed genes between propofol‑treated MCF7‑TR and untreated MCF7‑TR cells. Gene Ontology annotation enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Set Enrichment Analysis indicated that propofol affected the expression levels of genes located on the ‘plasma membrane’ and ‘cell periphery’, while mainly regulating signals involved in cancer biology, immune response and metabolic pathways. These results identified the potential effects of propofol on TR breast cancer cells and provided a theoretical basis for clinical treatment, particularly for individuals with TAM resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Yin R, Gao J, Liu Y and Guo C: Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation. Oncol Lett 29: 194, 2025.
APA
Yin, R., Gao, J., Liu, Y., & Guo, C. (2025). Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation. Oncology Letters, 29, 194. https://doi.org/10.3892/ol.2025.14940
MLA
Yin, R., Gao, J., Liu, Y., Guo, C."Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation". Oncology Letters 29.4 (2025): 194.
Chicago
Yin, R., Gao, J., Liu, Y., Guo, C."Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation". Oncology Letters 29, no. 4 (2025): 194. https://doi.org/10.3892/ol.2025.14940
Copy and paste a formatted citation
x
Spandidos Publications style
Yin R, Gao J, Liu Y and Guo C: Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation. Oncol Lett 29: 194, 2025.
APA
Yin, R., Gao, J., Liu, Y., & Guo, C. (2025). Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation. Oncology Letters, 29, 194. https://doi.org/10.3892/ol.2025.14940
MLA
Yin, R., Gao, J., Liu, Y., Guo, C."Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation". Oncology Letters 29.4 (2025): 194.
Chicago
Yin, R., Gao, J., Liu, Y., Guo, C."Functional analysis of the effects of propofol on tamoxifen‑resistant breast cancer cells: Insights into transcriptional regulation". Oncology Letters 29, no. 4 (2025): 194. https://doi.org/10.3892/ol.2025.14940
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