Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2025 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2025 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.pdf
Article Open Access

UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression

  • Authors:
    • Ai Watanabe
    • Jin Lu
    • Kai Ishihara
    • Sadahiro Iwabuchi
    • Kazuchika Ohno
    • Shinichi Hashimoto
    • Takahiro Asakage
    • Kazuki Takahashi
    • Katarzyna A. Podyma‑Inoue
    • Tetsuro Watabe
  • View Affiliations / Copyright

    Affiliations: Department of Head and Neck Surgery, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113‑8510, Japan, Department of Biochemistry, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo 113‑8510, Japan, Department of Molecular Pathophysiology, Wakayama Medical University, Wakayama 641‑8509, Japan
    Copyright: © Watanabe et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 473
    |
    Published online on: August 8, 2025
       https://doi.org/10.3892/ol.2025.15219
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Oral squamous cell carcinoma (OSCC) is a prevalent aggressive malignancy with a high mortality rate. However, the mechanisms underlying the progression of OSCC remain to be elucidated. In the present study, bioinformatics analysis identified ubiquitin‑conjugating enzyme E2 T (UBE2T) as a poor prognostic factor in head and neck cancer, showing the strongest association with cancer stage progression. Functional studies revealed that UBE2T can enhance motility and induce epithelial‑mesenchymal transition (EMT) in OSCC cells. RNA sequencing and subsequent analyses demonstrated that UBE2T upregulated the expression levels of various motility‑ and EMT‑related factors, including ankyrin repeat domain 1, endothelin‑1, interleukin‑6 (IL‑6), matrix metalloproteinase‑9 and plasminogen activator, urokinase. Gene set enrichment analysis indicated that UBE2T activates the IL‑6/Janus protein tyrosine kinase (JAK)/signal transducer and activator of transcription 3 signaling pathway. Moreover, treatment of OSCC cells with IL‑6 or a JAK inhibitor resulted in the induction of EMT and mesenchymal‑epithelial transition, respectively, accompanied by enhanced and suppressed cancer cell motility. These results indicated that IL‑6, which is upregulated by UBE2T, may be crucial for maintaining mesenchymal traits and motility in OSCC cells. Collectively, these findings suggested that the UBE2T/IL‑6/JAK axis may serve as a potential therapeutic target for OSCC.
View Figures

Figure 1

UBE2T correlates with poor prognosis
and progression of HNSCC. (A) Analysis of UBE2T expression in HNSCC
tumor (Tumor; red) and normal tissues (Normal; gray) using GEPIA
web server. Values represent first log2(TPM+1)
transformed expression data. HNSCC tumor tissues (n=519), normal
tissues (n=44). Statistical analysis: one-way ANOVA; *P<0.05.
(B) Correlation between UBE2T expression and OS of patients with
HNSCC. Kaplan-Meier survival plot of two patient cohorts with low
(black) and high (red) UBE2T expressions. HR and P-values are
shown. (C) Violin plots showing correlation between UBE2T
expression and pathological stages (stage I to IV) of HNSCC
analyzed using GEPIA web server. Values represent first
log2(TPM+1) transformed expression data. Statistical
analysis: one-way ANOVA; F and P values are shown. GEPIA, Gene
Expression Profiling Interactive Analysis; HNSCC, head and neck
squamous cell carcinoma; HR, hazard ratio; OS, overall survival;
TPM, transcripts per million; UBE2T, ubiquitin-conjugating enzyme
E2 T.

Figure 2

UBE2T enhances migration of oral
cancer cells. Ctrl SAS cells and UBE2T SAS cells (UBE2T) were
established by infecting SAS-Fucci cells with empty lentivirus or
lentivirus expressing FLAG-tagged UBE2T followed by chamber
migration assay. (A) Expression of FLAG-tagged UBE2T was visualized
by immunoblotting analysis using anti-FLAG M2 antibody. α-tubulin
was used as a loading control for cell lysate. (B and C) Migration
assay of Control and UBE2T SAS cells. (B) Representative images of
migrated cells are shown. Scale bars: 50 µm. (C) Quantitative
analysis of migrated cells. Data are represented as mean ± SD.
Statistical analysis: two-tailed unpaired Student's t-test;
****P<0.0001. Ctrl, control; UBE2T, ubiquitin-conjugating enzyme
E2 T.

Figure 3

UBE2T induces EMT in oral cancer
cells. (A and B) The expression levels of an epithelial cell marker
E-cadherin and a mesenchymal cell marker vimentin in Ctrl SAS cells
and UBE2T SAS cells (UBE2T) were examined using reverse
transcription-quantitative PCR. Relative expression of (A) an
epithelial cell marker, E-cadherin and (B) a mesenchymal cell
marker, vimentin. All data are normalized to the expression of
β-actin. Data are represented as mean ± SD. Statistical analyses:
two-tailed unpaired Student's t-test; ***P<0.001;
****P<0.0001. (C) GSEA of EMT gene signatures, comparing RNA
sequencing data from Ctrl SAS cells with UBE2T SAS cells (UBE2T).
n=2 per group. Ctrl, control; EMT, epithelial-mesenchymal
transition; GSEA, gene set enrichment analysis; NES, normalized
enrichment score; P-val, nominal P-value from GSEA; UBE2T,
ubiquitin-conjugating enzyme E2 T.

Figure 4

UBE2T upregulates the expression of
mesenchymal cell markers associated with motility and
epithelial-mesenchymal transition. (A) Gene Ontology functional
enrichment analysis of 62 differentially expressed genes
upregulated in UBE2T SAS cells compared with Ctrl SAS cells. (B-E)
Expression of various mesenchymal cell markers in Ctrl SAS cells
and UBE2T SAS cells (UBE2T) were examined by reverse
transcription-quantitative PCR. Relative expression of (B) ANKRD1,
(C) endothelin-1, (D) MMP-9 and (E) PLAU. All data are normalized
to the expression of β-actin. Data are represented as mean ± SD.
Statistical analyses: two-tailed unpaired Student's t-test;
**P<0.01; ****P<0.0001. ANKRD1, ankyrin repeat domain 1;
Ctrl, control; MMP-9, matrix metalloproteinase-9; PLAU, plasminogen
activator, urokinase; UBE2T, ubiquitin-conjugating enzyme E2 T.

Figure 5

Expressions of IL-6 and genes
involved in IL-6-related signaling are upregulated in UBE2T SAS
cells. (A) Search Tool for the Retrieval of Interacting Genes
analysis of 62 differentially expressed genes upregulated in UBE2T
SAS cells. Red font indicates previously reported factors,
associated with cancer cell migration and epithelial-mesenchymal
transition. (B) GSEA of ‘HALLMARK_IL6_JAK_STAT3_SIGNALING’ gene set
comparing RNA sequencing data from Ctrl SAS cells and UBE2T SAS
cells (UBE2T). n=2 per group. (C) Relative expression of IL-6 in
Control SAS cells (Ctrl) and UBE2T SAS cells (UBE2T). Data are
normalized to the expression of β-actin. Data are represented as
mean ± SD. Statistical analysis: two-tailed unpaired Student's
t-test; ****P<0.0001. ANKRD1, ankyrin repeat domain 1; Ctrl,
control; EDN1, endothelin-1; GSEA, gene set enrichment analysis;
IL-6/IL6, interleukin-6; JAK, Janus protein tyrosine kinase; MMP9,
matrix metalloproteinase-9; NES, normalized enrichment score; PLAU,
plasminogen activator, urokinase; P, nominal P-value from GSEA;
STAT3, signal transducer and activator of transcription 3; UBE2T,
ubiquitin-conjugating enzyme E2 T; VIM, vimentin.

Figure 6

IL-6 induces epithelial-mesenchymal
transition and enhances migration of oral cancer cells. SAS cells
were cultured in the absence (Ctrl) or presence of IL-6 (IL-6) for
72 h followed by (A and B) reverse transcription-quantitative PCR
and (C and D) chamber migration assay. Relative expression levels
of (A) an epithelial cell marker, E-cadherin and (B) a mesenchymal
cell marker, vimentin. All data are normalized to the expression of
β-actin. (C) Representative images of migrated cells treated
without (Ctrl) or with IL-6 (IL-6) are shown. Scale bars: 50 µm.
(D) Quantitative analysis of migrated cells. All data are
represented as mean ± SD. Statistical analyses: two-tailed unpaired
Student's t-test; **P<0.01, ****P<0.0001. Ctrl, control;
IL-6, interleukin-6.

Figure 7

JAKi induces mesenchymal-epithelial
transition and suppresses migration of oral cancer cells. UBE2T SAS
cells were cultured in the absence (Ctrl) or presence of
ruxolitinib, a JAKi for 72 h followed by (A and B) reverse
transcription-quantitative PCR and (C and D) chamber migration
assay. Relative expression levels of (A) an epithelial cell marker,
E-cadherin and (B) a mesenchymal cell marker, vimentin. All data
are normalized to the expression of β-actin. (C) Representative
images of migrated cells treated without (Ctrl) or with JAKi are
shown. Scale bars: 50 µm. (D) Quantitative analysis of migrated
cells. All data are represented as mean ± SD. Statistical analyses:
two-tailed unpaired Student's t-test; **P<0.01, ***P<0.001.
Ctrl, control; JAKi, Janus protein tyrosine kinase inhibitor.
View References

1 

Biray Avci C, Goker Bagca B, Nikanfar M, Takanlou LS, Takanlou MS and Nourazarian A: Tumor microenvironment and cancer metastasis: Molecular mechanisms and therapeutic implications. Front Pharmacol. 15:14428882024. View Article : Google Scholar : PubMed/NCBI

2 

Li Y, Liu F, Cai Q, Deng L, Ouyang Q, Zhang XH and Zheng J: Invasion and metastasis in cancer: Molecular insights and therapeutic targets. Sig Transduct Target Ther. 10:572025. View Article : Google Scholar : PubMed/NCBI

3 

Miyazono K, Katsuno Y, Koinuma D, Ehata S and Morikawa M: Intracellular and extracellular TGF-β signaling in cancer: Some recent topics. Front Med. 12:387–411. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Xie Y, Wang X, Wang W, Pu N and Liu L: Epithelial-mesenchymal transition orchestrates tumor microenvironment: Current perceptions and challenges. J Transl Med. 23:3862025. View Article : Google Scholar : PubMed/NCBI

5 

Robinson DR, Wu YM, Lonigro RJ, Vats P, Cobain E, Everett J, Cao X, Rabban E, Kumar-Sinha C, Raymond V, et al: Integrative clinical genomics of metastatic cancer. Nature. 548:297–303. 2017. View Article : Google Scholar : PubMed/NCBI

6 

Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I and Jemal A: Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 74:229–263. 2024.PubMed/NCBI

7 

Hernández-Morales A, González-López BS, Scougall-Vilchis RJ, Bermeo-Escalona JR, Velázquez-Enríquez U, Islas-Zarazúa R, Márquez-Rodríguez S, Sosa-Velasco TA, Medina-Solís CE and Maupomé G: Lip and oral cavity cancer incidence and mortality rates associated with smoking and chewing tobacco use and the human development index in 172 countries worldwide: An ecological study 2019–2020. Healthcare (Basel). 11:10632023. View Article : Google Scholar : PubMed/NCBI

8 

Kinoshita N, Tomioka H, Oikawa Y, Fukawa Y, Ikeda T and Harada H: A case of sclerosing odontogenic carcinoma of the mandible with a review of the literature. J Oral Sci. 65:281–283. 2023. View Article : Google Scholar : PubMed/NCBI

9 

Tranby EP, Heaton LJ, Tomar SL, Kelly AL, Fager GL, Backley M and Frantsve-Hawley J: Oral cancer prevalence, mortality, and costs in Medicaid and commercial insurance claims data. Cancer Epidemiol Biomarkers Prev. 31:1849–1857. 2022. View Article : Google Scholar : PubMed/NCBI

10 

Miranda-Filho A and Bray F: Global patterns and trends in cancers of the lip, tongue and mouth. Oral Oncol. 102:1045512020. View Article : Google Scholar : PubMed/NCBI

11 

Tan Y, Wang Z, Xu M, Li B, Huang Z, Qin S, Nice EC, Tang J and Huang C: Oral squamous cell carcinomas: State of the field and emerging directions. Int J Oral Sci. 15:442023. View Article : Google Scholar : PubMed/NCBI

12 

Katsuno Y and Derynck R: Epithelial plasticity, epithelial-mesenchymal transition, and the TGF-β family. Dev Cell. 56:726–746. 2021. View Article : Google Scholar : PubMed/NCBI

13 

Ling H, Xiao S, Lei Y, Zhou Y, Tan J, Chen X, Ma D, Liang C, Liu Q, Liu W and Zeng T: The advancement of ubiquitination regulation in apoptosis, ferroptosis, autophagy, drug resistance and treatment of cancer. Arch Biochem Biophys. 771:1104972025. View Article : Google Scholar : PubMed/NCBI

14 

Wang Y, Gao G, Wei X, Zhang Y and Yu J: UBE2T promotes temozolomide resistance of glioblastoma through regulating the Wnt/β-catenin signaling pathway. Drug Des Devel Ther. 17:1357–1369. 2023. View Article : Google Scholar : PubMed/NCBI

15 

Peng J, Dai X, Zhang T, Hu G, Cao H, Guo X, Fan H, Chen J, Tang W and Yang F: Copper as the driver of the lncRNA-TCONS-6251/miR-novel-100/TC2N axis: Unraveling ferroptosis in duck kidney. Int J Biol Macromol. 282((Pt 2)): 1367972024. View Article : Google Scholar : PubMed/NCBI

16 

Chen J, Dai X, Xing C, Zhang Y, Cao H, Hu G, Guo X, Gao X, Liu P and Yang F: Cooperative application of transcriptomics and ceRNA hypothesis: lncRNA-00742/miR-116 targets CD74 to mediate vanadium-induced mitochondrial apoptosis in duck liver. J Hazard Mater. 480:1359042024. View Article : Google Scholar : PubMed/NCBI

17 

Liu F, Chen J, Li K, Li H, Zhu Y, Zhai Y, Lu B, Fan Y, Liu Z, Chen X, et al: Ubiquitination and deubiquitination in cancer: From mechanisms to novel therapeutic approaches. Mol Cancer. 23:1482024. View Article : Google Scholar : PubMed/NCBI

18 

Dutta R, Guruvaiah P, Reddi KK, Bugide S, Reddy Bandi DS, Edwards YJK, Singh K and Gupta R: UBE2T promotes breast cancer tumor growth by suppressing DNA replication stress. NAR Cancer. 4:zcac0352022. View Article : Google Scholar : PubMed/NCBI

19 

Ma N, Li Z, Yan J, Liu X, He L, Xie R and Lu X: Diverse roles of UBE2T in cancer (Review). Oncol Rep. 49:692023. View Article : Google Scholar : PubMed/NCBI

20 

Yin H, Wang X, Zhang X, Zeng Y, Xu Q, Wang W, Zhou F and Zhou Y: UBE2T promotes radiation resistance in non-small cell lung cancer via inducing epithelial-mesenchymal transition and the ubiquitination-mediated FOXO1 degradation. Cancer Lett. 494:121–131. 2020. View Article : Google Scholar : PubMed/NCBI

21 

Ho NPY, Leung CON, Wong TL, Lau EYT, Lei MML, Mok EHK, Leung HW, Tong M, Ng IOL, Yun JP, et al: The interplay of UBE2T and Mule in regulating Wnt/β-catenin activation to promote hepatocellular carcinoma progression. Cell Death Dis. 12:1482021. View Article : Google Scholar : PubMed/NCBI

22 

Hao P, Kang B, Li Y, Hao W and Ma F: UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma. Mol Med Rep. 20:1212–1220. 2019.PubMed/NCBI

23 

Kim JW, Gautam J, Kim JE, Kim JA and Kang KW: Inhibition of tumor growth and angiogenesis of tamoxifen-resistant breast cancer cells by ruxolitinib, a selective JAK2 inhibitor. Oncol Lett. 17:3981–3989. 2019.PubMed/NCBI

24 

Bai Y, Wang W, Yin P, Gao J, Na L, Sun Y, Wang Z, Zhang Z and Zhao C: Ruxolitinib alleviates renal interstitial fibrosis in UUO Mice. Int J Biol Sci. 16:194–203. 2020. View Article : Google Scholar : PubMed/NCBI

25 

Onozato Y, Kaida A, Harada H and Miura M: Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108:704–712. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, et al: Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell. 132:487–498. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Tang Z, Li C, Kang B, Gao G, Li C and Zhang Z: GEPIA: A web server for cancer and normal gene expression profiling and interactive analyses. Nucleic Acids Res. 45((W1)): W98–W102. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Győrffy B: Integrated analysis of public datasets for the discovery and validation of survival-associated genes in solid tumors. Innovation (Camb). 5:1006252024.PubMed/NCBI

29 

Miyoshi H, Blömer U, Takahashi M, Gage FH and Verma IM: Development of a self-inactivating lentivirus vector. J Virol. 72:8150–8157. 1998. View Article : Google Scholar : PubMed/NCBI

30 

Kodama S, Podyma-Inoue KΑ, Uchihashi T, Kurioka K, Takahashi H, Sugauchi A, Takahashi K, Inubushi T, Kogo M, Tanaka S and Watabe T: Progression of melanoma is suppressed by targeting all transforming growth factor-β isoforms with an Fc chimeric receptor. Oncol Rep. 46:1972021. View Article : Google Scholar : PubMed/NCBI

31 

Takahashi K, Akatsu Y, Podyma-Inoue KA, Matsumoto T, Takahashi H, Yoshimatsu Y, Koinuma D, Shirouzu M, Miyazono K and Watabe T: Targeting all transforming growth factor-β isoforms with an Fc chimeric receptor impairs tumor growth and angiogenesis of oral squamous cell cancer. J Biol Chem. 295:12559–12572. 2020. View Article : Google Scholar : PubMed/NCBI

32 

Schneider CA, Rasband WS and Eliceiri KW: NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Pfaffl MW: Relative quantification. Real-time PCR and Dorak MT: 1st edition. Taylor & Francis; London: 2006

34 

Mootha VK, Lindgren CM, Eriksson KF, Subramanian A, Sihag S, Lehar J, Puigserver P, Carlsson E, Ridderstråle M, Laurila E, et al: PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet. 34:267–273. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Subramanian A, Tamayo P, Mootha VK, Mukherjee S, Ebert BL, Gillette MA, Paulovich A, Pomeroy SL, Golub TR, Lander ES and Mesirov JP: Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA. 102:15545–15550. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC and Lempicki RA: DAVID: Database for annotation, visualization, and integrated discovery. Genome Biol. 4:P32003. View Article : Google Scholar : PubMed/NCBI

37 

Szklarczyk D, Franceschini A, Wyder S, Forslund K, Heller D, Huerta-Cepas J, Simonovic M, Roth A, Santos A, Tsafou KP, et al: STRING v10: Protein-protein interaction networks, integrated over the tree of life. Nucleic Acids Res. 43((Database issue)): D447–D452. 2015. View Article : Google Scholar : PubMed/NCBI

38 

Xu X, Zhong D, Wang X, Luo F, Zheng X, Feng T, Chen R, Cheng Y, Wang Y and Ma G: Pan-cancer integrated analysis of ANKRD1 expression, prognostic value, and potential implications in cancer. Sci Rep. 14:52682024. View Article : Google Scholar : PubMed/NCBI

39 

Harrison M, Zinovkin D and Pranjol MZI: Endothelin-1 and its role in cancer and potential therapeutic opportunities. Biomedicines. 12:5112024. View Article : Google Scholar : PubMed/NCBI

40 

Ibi H, Takahashi K, Harada H, Watabe T and Podyma-Inoue KA: Transforming growth factor-β signals promote progression of squamous cell carcinoma by inducing epithelial-mesenchymal transition and angiogenesis. Biochem Biophys Res Commun. 714:1499652024. View Article : Google Scholar : PubMed/NCBI

41 

Sarno F, Goubert D, Logie E, Rutten MGS, Koncz M, Deben C, Niemarkt AE, Altucci L, Verschure PJ, Kiss A, et al: Functional validation of the putative oncogenic activity of PLAU. Biomedicines. 11:1022022. View Article : Google Scholar : PubMed/NCBI

42 

Yadav A, Kumar B, Datta J, Teknos TN and Kumar P: IL-6 promotes head and neck tumor metastasis by inducing epithelial-mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway. Mol Cancer Res. 9:1658–1667. 2011. View Article : Google Scholar : PubMed/NCBI

43 

Thuya WL, Cao Y, Ho PC, Wong AL, Wang L, Zhou J, Nicot C and Goh BC: Insights into IL-6/JAK/STAT3 signaling in the tumor microenvironment: Implications for cancer therapy. Cytokine Growth Factor Rev. Jan 17–2025.(Epub ahead of print). View Article : Google Scholar : PubMed/NCBI

44 

Gao C, Liu YJ, Yu J, Wang R, Shi JJ, Chen RY, Yang GJ and Chen J: Unraveling the role of ubiquitin-conjugating enzyme UBE2T in tumorigenesis: A comprehensive review. Cells. 4:152024. View Article : Google Scholar

45 

Augoff K, Hryniewicz-Jankowska A, Tabola R and Stach K: MMP9: A tough target for targeted therapy for cancer. Cancers (Basel). 14:18472022. View Article : Google Scholar : PubMed/NCBI

46 

Kumari N, Dwarakanath BS, Das A and Bhatt AN: Role of interleukin-6 in cancer progression and therapeutic resistance. Tumour Biol. 37:11553–11572. 2016. View Article : Google Scholar : PubMed/NCBI

47 

Cai F, Xu H, Song S, Wang G, Zhang Y, Qian J and Xu L: Knockdown of ubiquitin-conjugating enzyme E2 T abolishes the progression of head and neck squamous cell carcinoma by inhibiting NF-κb signaling and inducing ferroptosis. Curr Protein Pept Sci. 25:577–585. 2024. View Article : Google Scholar : PubMed/NCBI

48 

Yu Z, Jiang X, Qin L, Deng H, Wang J, Ren W, Li H, Zhao L, Liu H, Yan H, et al: A novel UBE2T inhibitor suppresses Wnt/β-catenin signaling hyperactivation and gastric cancer progression by blocking RACK1 ubiquitination. Oncogene. 40:1027–1042. 2021. View Article : Google Scholar : PubMed/NCBI

49 

Pu J, Wang B, Zhang D, Wang K, Yang Z, Zhu P and Song Q: UBE2T mediates SORBS3 ubiquitination to enhance IL-6/STAT3 signaling and promote lung adenocarcinoma progression. J Biochem Mol Toxicol. 38:e237432024. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Watanabe A, Lu J, Ishihara K, Iwabuchi S, Ohno K, Hashimoto S, Asakage T, Takahashi K, Podyma‑Inoue KA, Watabe T, Watabe T, et al: UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression. Oncol Lett 30: 473, 2025.
APA
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S. ... Watabe, T. (2025). UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression. Oncology Letters, 30, 473. https://doi.org/10.3892/ol.2025.15219
MLA
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S., Asakage, T., Takahashi, K., Podyma‑Inoue, K. A., Watabe, T."UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression". Oncology Letters 30.4 (2025): 473.
Chicago
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S., Asakage, T., Takahashi, K., Podyma‑Inoue, K. A., Watabe, T."UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression". Oncology Letters 30, no. 4 (2025): 473. https://doi.org/10.3892/ol.2025.15219
Copy and paste a formatted citation
x
Spandidos Publications style
Watanabe A, Lu J, Ishihara K, Iwabuchi S, Ohno K, Hashimoto S, Asakage T, Takahashi K, Podyma‑Inoue KA, Watabe T, Watabe T, et al: UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression. Oncol Lett 30: 473, 2025.
APA
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S. ... Watabe, T. (2025). UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression. Oncology Letters, 30, 473. https://doi.org/10.3892/ol.2025.15219
MLA
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S., Asakage, T., Takahashi, K., Podyma‑Inoue, K. A., Watabe, T."UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression". Oncology Letters 30.4 (2025): 473.
Chicago
Watanabe, A., Lu, J., Ishihara, K., Iwabuchi, S., Ohno, K., Hashimoto, S., Asakage, T., Takahashi, K., Podyma‑Inoue, K. A., Watabe, T."UBE2T promotes epithelial‑mesenchymal transition and motility in oral cancer cells via induction of IL‑6 expression". Oncology Letters 30, no. 4 (2025): 473. https://doi.org/10.3892/ol.2025.15219
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team