Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
October-2025 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2025 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data1.pdf
    • Supplementary_Data2.pdf
Article Open Access

Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level

  • Authors:
    • Runyang Yin
    • Rui Xu
    • Hui Ming
    • Juntao Song
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China, Department of Oncology Hematology, Zibo 148 Hospital, Zibo, Shandong 255399, P.R. China, Department of Anesthesiology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia Autonomous Region 010050, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 489
    |
    Published online on: August 19, 2025
       https://doi.org/10.3892/ol.2025.15235
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Acute myeloid leukemia (AML), which is characterized by the aberrant proliferation of primitive and immature myelocytes within the bone marrow, represents the most prevalent subtype of leukemia in both adults and children. Isoimperatorin, a derivative of coumarins, has been reported to possess antitumor, antioxidative and anti‑inflammatory activities. In the present study, the effects of isoimperatorin on human AML cells were evaluated. Tohoku Hospital Pediatrics‑1 (THP‑1) and AML‑193 cells were treated with or without isoimperatorin. Subsequently, induction of apoptosis was detected by flow cytometry, whereas cell proliferation was assessed using Cell Counting Kit‑8. Furthermore, the potential transcriptional regulatory effects of isoimperatorin on THP‑1 cells were investigated using RNA sequencing and reverse transcription‑quantitative PCR (RT‑qPCR). Isoimperatorin significantly induced apoptosis and inhibited cell proliferation in the AML cells. Furthermore, transcriptome sequencing analysis demonstrated 688 differentially expressed genes between isoimperatorin‑treated THP‑1 cells and untreated controls. Gene Ontology annotation enrichment analysis, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene Set Enrichment Analysis indicated that isoimperatorin altered the expression genes associated with ‘cell migration’ and ‘cell motility’. The compound primarily regulated signaling pathways, including the ‘calcium signaling pathway’, ‘microRNA in cancer’ and ‘MAPK signaling pathway’, while influencing biological processes related to ‘cancer biology’, ‘metabolic pathways’ and ‘immune responses’. These findings were verified using RT‑qPCR. Collectively, the present results elucidated the potential effects of isoimperatorin on AML and provided a theoretical foundation for the clinical treatment of AML.
View Figures

Figure 1

Isoimperatorin treatment
significantly promotes apoptosis and suppresses proliferation in
AML cells. (A) Isoimperatorin treatment significantly increased the
apoptosis of THP-1 cells. THP-1 cells were treated with or without
12.5, 25 or 50 µM isoimperatorin for 24 h, stained with annexin V
and PI, and subjected to apoptosis detection using flow cytometry.
(B) Percentage of annexin V+ and PI− early
apoptotic cells. (C) Percentage of annexin V+ and
PI+ late apoptotic cells. (D) Isoimperatorin
significantly inhibited the proliferation of THP-1 cells. (E)
Isoimperatorin treatment significantly increased the apoptosis of
AML-193 cells. AML-193 cells were treated with or without 15, 30 or
60 µM isoimperatorin for 24 h, stained with annexin V and PI, and
subjected to apoptosis analysis via flow cytometry. (F) Percentage
of annexin V+ and PI− early apoptotic AML-193
cells. (G) Percentage of annexin V+ and PI+
late apoptotic AML-193 cells. (H) Isoimperatorin significantly
inhibited the proliferation of AML-193 cells Data are presented as
the mean ± SD. Significant differences were examined using one-way
analysis of variance with Tukey's post hoc test in panels (B), (C),
(F) and (G) and two-way analysis of variance with Tukey's post hoc
test in panels (D) and (H). **P<0.01 and ***P<0.001. AML,
acute myeloid leukemia; CCK-8, Cell Counting Kit-8; THP-1, Tohoku
Hospital Pediatrics-1.

Figure 2

Isoimperatorin treatment affects gene
expression profiles in AML cells. (A) Principal component analysis
was performed to show inter-group differences and intra-group
consistency. (B) Two-way cluster analysis of DEGs between
isoimperatorin-treated and untreated THP-1 cells was performed
using the pheatmap package. (C) Volcano plot indicating DEGs
between isoimperatorin-treated and untreated THP-1 cells. (D)
Scatter plot of the top 20 most significantly enriched GO terms
(biological processes, cellular components and molecular functions)
for DEGs in THP-1 cells after treatment with 12.5 µM isoimperatorin
for 24 h. (E) Scatter plot of the top 20 most significantly
enriched KEGG signaling pathways obtained from the RNA sequencing
data of THP-1 cells after treatment with 12.5 µM isoimperatorin for
24 h. The degree of enrichment was indicated by the rich factor,
FDR and number of genes. AML, acute myeloid leukemia; DEGs,
differentially expressed genes; THP-1, Tohoku Hospital
Pediatrics-1; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes
and Genomes; FDR, false discovery rate; C represents the control; T
represents THP-1 cells treated.

Figure 3

Isoimperatorin treatment affects gene
expression profiles associated with tumor biology processes in AML
cells. Heatmaps indicated that differentially expressed genes in
THP-1 cells treated with isoimperatorin were involved in (A)
‘microRNAs in cancer’, (B) ‘prostate cancer’, (C) ‘small cell lung
cancer’ and (D) ‘proteoglycans in cancer’. From each pathway, two
upregulated and downregulated genes in (E) ‘microRNAs in cancer’,
(F) ‘prostate cancer’, (G) ‘small cell lung cancer’ and (H)
‘proteoglycans in cancer’ processes were selected and detected by
reverse transcription-quantitative PCR analysis in THP-1 cells.
Data are presented as the mean ± SD. Significant differences were
examined using unpaired two-tailed Student's t-test in panels
(E)-(H). **P<0.01 and ***P<0.001. THP-1, Tohoku Hospital
Pediatrics-1. C represents the control samples, with THP-1_C1,
THP-1_C2, and THP-1_C3 as biological replicates. THP-1_T represents
THP-1 cells treated with 12.5 µM isoimperatorin, including
THP-1_T1, THP-1_T2, and THP-1_T3 as biological replicates.

Figure 4

Isoimperatorin treatment affects gene
expression profiles associated with the metabolic processes in AML
cells. Heatmaps indicated that differentially expressed genes in
THP-1 cells treated with isoimperatorin were involved in the (A)
‘glycosaminoglycan biosynthesis’, (B) ‘mucin type O-glycan
biosynthesis’, (C) ‘steroid hormone biosynthesis’ and (D)
‘glycosphingolipid biosynthesis’ pathways. From each pathway, two
upregulated and downregulated genes in (E) ‘glycosaminoglycan
biosynthesis’, (F) ‘mucin type O-glycan biosynthesis’, (G) ‘steroid
hormone biosynthesis’ and (H) ‘glycosphingolipid biosynthesis’
pathways were selected and detected by reverse
transcription-quantitative PCR analysis in THP-1 cells. Data are
presented as the mean ± SD. Significant differences were examined
using unpaired two-tailed Student's t-test in panels (E)-(H).
**P<0.01 and ***P<0.001. THP-1, Tohoku Hospital Pediatrics-1.
C represents the control samples, with THP-1_C1, THP-1_C2, and
THP-1_C3 as biological replicates. THP-1_T represents THP-1 cells
treated with 12.5 µM isoimperatorin, including THP-1_T1, THP-1_T2,
and THP-1_T3 as biological replicates.

Figure 5

Isoimperatorin treatment affects gene
expression profiles associated with the immune response system in
AML cells. Heatmaps indicated that differentially expressed genes
in THP-1 cells treated with isoimperatorin were involved in (A)
‘chemokine signaling pathway’, (B) ‘Th1 and Th2 cell
differentiation’, (C) ‘NOD-like receptor signaling pathway’ and (D)
‘leukocyte transendothelial migration’. From each pathway, two
upregulated and downregulated genes in (E) ‘chemokine signaling
pathway’, (F) ‘Th1 and Th2 cell differentiation’, (G) ‘NOD-like
receptor signaling pathway’ and (H) ‘leukocyte transendothelial
migration’ processes were selected and evaluated by reverse
transcription-quantitative PCR analysis in THP-1 cells. Data are
presented as the mean ± SD. Significant differences were examined
using unpaired two-tailed Student's t-test in panels (E)-(H).
**P<0.01 and ***P<0.001. THP-1, Tohoku Hospital Pediatrics-1.
C represents the control samples, with THP-1_C1, THP-1_C2, and
THP-1_C3 as biological replicates. THP-1_T represents THP-1 cells
treated with 12.5 µM isoimperatorin, including THP-1_T1, THP-1_T2,
and THP-1_T3 as biological replicates.

Figure 6

Signaling pathways involved in the
sensitivity of THP-1 cells to isoimperatorin. Gene Set Enrichment
Analysis plots indicated that differentially expressed genes in
THP-1 cells treated with isoimperatorin were involved in (A) the
‘Rig-I-like receptor signaling pathway’, (B) ‘legionellosis’, (C)
‘ribosome’ and (D) ‘glacoseminoglycan biosynthesis-heparan sulfate
heparin’. ES, enrichment score; THP-1, Tohoku Hospital
Pediatrics-1.
View References

1 

Whiteley AE, Price TT, Cantelli G and Sipkins DA: Leukaemia: A model metastatic disease. Nat Rev Cancer. 21:461–475. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Kantarjian HM, DiNardo CD, Kadia TM, Daver NG, Altman JK, Stein EM, Jabbour E, Schiffer CA, Lang A and Ravandi F: Acute myeloid leukemia management and research in 2025. CA Cancer J Clin. 75:46–67. 2025.PubMed/NCBI

3 

Villeneuve P, Kim DT, Xu W, Brandwein J and Chang H: The morphological subcategories of acute monocytic leukemia (M5a and M5b) share similar immunophenotypic and cytogenetic features and clinical outcomes. Leuk Res. 32:269–273. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Botta L, Gatta G, Capocaccia R, Stiller C, Cañete A, Dal Maso L, Innos K, Mihor A, Erdmann F, Spix C, et al: Long-term survival and cure fraction estimates for childhood cancer in Europe (EUROCARE-6): Results from a population-based study. Lancet Oncol. 23:1525–1536. 2022. View Article : Google Scholar : PubMed/NCBI

5 

Yu WJ, Sun YQ, Xu LP, Zhang XH, Liu KY, Huang XJ and Wang Y: Comparison of outcomes for patients with acute myeloid leukemia undergoing haploidentical stem cell transplantation in first and second complete remission. Ann Hematol. 102:2241–2250. 2023. View Article : Google Scholar : PubMed/NCBI

6 

Saliba RM, Kanakry CG, Gadalla S, Kebriaei P, Rezvani K, Champlin RE, Shpall EJ, Weisdorf D and Mehta RS: Effect of donor age in patients with acute myeloid leukemia undergoing haploidentical hematopoietic cell transplantation vary by conditioning intensity and recipient age. Am J Hematol. 99:38–47. 2024. View Article : Google Scholar : PubMed/NCBI

7 

Peroni E, Randi ML, Rosato A and Cagnin S: Acute myeloid leukemia: from NGS, through scRNA-seq, to CAR-T. dissect cancer heterogeneity and tailor the treatment. J Exp Clin Cancer Res. 42:2592023. View Article : Google Scholar : PubMed/NCBI

8 

Gabellier L, Bosetta E, Heiblig M and Sarry JE: Metabolism and therapeutic response in acute myeloid leukemia with IDH1/2 mutations. Trends Cancer. 11:475–490. 2025. View Article : Google Scholar : PubMed/NCBI

9 

Soleimani Samarkhazan H, Zehtabcheh S, Seraji HR, Beqaj SH, Tayefeh S, Mohammadi MH and Aghaei M: Unveiling the potential of CLL-1: A promising target for AML therapy. Biomark Res. 13:282025. View Article : Google Scholar : PubMed/NCBI

10 

Brivio E, Baruchel A, Beishuizen A, Bourquin JP, Brown PA, Cooper T, Gore L, Kolb EA, Locatelli F, Maude SL, et al: Targeted inhibitors and antibody immunotherapies: Novel therapies for paediatric leukaemia and lymphoma. Eur J Cancer. 164:1–17. 2022. View Article : Google Scholar : PubMed/NCBI

11 

Roas M, Vick B, Kasper MA, Able M, Polzer H, Gerlach M, Kremmer E, Hecker JS, Schmitt S, Stengl A, et al: Targeting FLT3 with a new-generation antibody-drug conjugate in combination with kinase inhibitors for treatment of AML. Blood. 141:1023–1035. 2023. View Article : Google Scholar : PubMed/NCBI

12 

Huls GA, Woolthuis CM and Schuringa JJ: Menin inhibitors in the treatment of acute myeloid leukemia. Blood. 145:561–566. 2025. View Article : Google Scholar : PubMed/NCBI

13 

Venugopal S and Sekeres MA: Contemporary management of acute myeloid leukemia: A review. JAMA Oncol. 10:1417–1425. 2024. View Article : Google Scholar : PubMed/NCBI

14 

Wu RH, Zhu CY, Yu PH, Ma Y, Hussain L, Naranmandura H and Wang QQ: The landscape of novel strategies for acute myeloid leukemia treatment: Therapeutic trends, challenges, and future directions. Toxicol Appl Pharmacol. 473:1165852023. View Article : Google Scholar : PubMed/NCBI

15 

Peroni E, Gottardi M, D'Antona L, Randi ML, Rosato A and Coltro G: Hematologic Neoplasms associated with down syndrome: Cellular and molecular heterogeneity of the diseases. Int J Mol Sci. 24:153252023. View Article : Google Scholar : PubMed/NCBI

16 

Martinez TC and McNerney ME: Haploinsufficient transcription factors in myeloid neoplasms. Annu Rev Pathol. 19:571–598. 2024. View Article : Google Scholar : PubMed/NCBI

17 

Mishra SK, Millman SE and Zhang L: Metabolism in acute myeloid leukemia: Mechanistic insights and therapeutic targets. Blood. 141:1119–1135. 2023. View Article : Google Scholar : PubMed/NCBI

18 

Smoljo T, Tomic B, Lalic H, Dembitz V, Batinic J, Bedalov A and Visnjic D: Bone marrow stromal cells reduce low-dose cytarabine-induced differentiation of acute myeloid leukemia. Front Pharmacol. 14:12581512023. View Article : Google Scholar : PubMed/NCBI

19 

Thuy NTT, Lee JE, Yoo HM and Cho N: Antiproliferative pterocarpans and coumestans from lespedeza bicolor. J Nat Prod. 82:3025–3032. 2019. View Article : Google Scholar : PubMed/NCBI

20 

Fan L, Li Z, Gao L, Zhang N and Chang W: Isoimperatorin alleviates lipopolysaccharide-induced periodontitis by downregulating ERK1/2 and NF-κB pathways. Open Life Sci. 18:202205412023. View Article : Google Scholar : PubMed/NCBI

21 

Ko HJ, Park SY, Sim DY, Kim SH, Hur S, Lee JH and Kim Y: Apoptotic effect of isoimpertorin via inhibition of c-Myc and SIRT1 signaling axis. Int J Mol Sci. 25:42482024. View Article : Google Scholar : PubMed/NCBI

22 

Kim NY, Jung YY, Yang MH, Um JY, Sethi G and Ahn KS: Isoimperatorin down-regulates epithelial mesenchymal transition through modulating NF-κB signaling and CXCR4 expression in colorectal and hepatocellular carcinoma cells. Cell Signal. 99:1104332022. View Article : Google Scholar : PubMed/NCBI

23 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Zhou Y, Huang G, Cai X, Liu Y, Qian B and Li D: Global, regional, and national burden of acute myeloid leukemia, 1990–2021: A systematic analysis for the global burden of disease study 2021. Biomark Res. 12:1012024. View Article : Google Scholar : PubMed/NCBI

25 

Gaur T, Ali A, Sharma D, Gupta SK, Gota V, Bagal B, Platzbeckar U, Mishra R, Dutt A, Khattry N, et al: Mitocurcumin utilizes oxidative stress to upregulate JNK/p38 signaling and overcomes Cytarabine resistance in acute myeloid leukemia. Cell Signal. 114:1110042024. View Article : Google Scholar : PubMed/NCBI

26 

Xu M and Li S: The opportunities and challenges of using PD-1/PD-L1 inhibitors for leukemia treatment. Cancer Lett. 593:2169692024. View Article : Google Scholar : PubMed/NCBI

27 

Liu Q, Guan Y and Li S: Programmed death receptor (PD-)1/PD-ligand (L)1 in urological cancers: The ‘all-around warrior’ in immunotherapy. Mol Cancer. 23:1832024. View Article : Google Scholar : PubMed/NCBI

28 

Alsouqi A, Geramita E and Im A: Treatment of acute myeloid leukemia in older adults. Cancers (Basel). 15:54092023. View Article : Google Scholar : PubMed/NCBI

29 

Buchrits S and Wolach O: Non-immunotherapy approaches for Relapsed or Refractory AML: An update for 2024. Acta Haematol. 147:159–174. 2024. View Article : Google Scholar : PubMed/NCBI

30 

Xiao X, Wang P, Zhang W, Wang J, Cai M, Jiang H, Wu Y and Shan H: GNF-7, a novel FLT3 inhibitor, overcomes drug resistance for the treatment of FLT3-ITD acute myeloid leukemia. Cancer Cell Int. 23:3022023. View Article : Google Scholar : PubMed/NCBI

31 

Chen J, Zhou X, Fu L and Xu H: Natural product-based screening for lead compounds targeting SARS CoV-2 Mpro. Pharmaceuticals (Basel). 16:7672023. View Article : Google Scholar : PubMed/NCBI

32 

Yao L, Cai W, Chen S, Wang A, Wang X, Zhao C, Shou C and Jia Y: Design, syntheses and biological evaluation of natural product aiphanol derivatives and analogues: Discovery of potent anticancer agents. Bioorg Med Chem Lett. 90:1293262023. View Article : Google Scholar : PubMed/NCBI

33 

Rajendran P, Althumairy D, Bani-Ismail M, Bekhet GM and Ahmed EA: Isoimperatorin therapeutic effect against aluminum induced neurotoxicity in albino mice. Front Pharmacol. 14:11039402023. View Article : Google Scholar : PubMed/NCBI

34 

Skwarska A and Konopleva M: BCL-xL targeting to induce apoptosis and to eliminate chemotherapy-induced senescent tumor cells: From navitoclax to platelet-sparing BCL-xL PROTACs. Cancer Res. 83:3501–3503. 2023. View Article : Google Scholar : PubMed/NCBI

35 

Liu J, He L, Hu J, Li K, Zhou F, Hu M, Luo J, Song L and He Y: Isoimperatorin induces apoptosis of nasopharyngeal carcinoma cells via the MAPK/ERK1/2 signaling pathway. Evid Based Complement Alternat Med. 2020:21381862020. View Article : Google Scholar : PubMed/NCBI

36 

Gruszka AM, Valli D, Restelli C and Alcalay M: Adhesion deregulation in acute myeloid leukaemia. Cells. 8:662019. View Article : Google Scholar : PubMed/NCBI

37 

Pereira RS, Kumar R, Cais A, Paulini L, Kahler A, Bravo J, Minciacchi VR, Krack T, Kowarz E, Zanetti C, et al: Distinct and targetable role of calcium-sensing receptor in leukaemia. Nat Commun. 14:62422023. View Article : Google Scholar : PubMed/NCBI

38 

Xiao J, Zhang B, Yin S, Xie S, Huang K, Wang J, Yang W, Liu H, Zhang G, Liu X, et al: Quercetin induces autophagy-associated death in HL-60 cells through CaMKKβ/AMPK/mTOR signal pathway. Acta Biochim Biophys Sin (Shanghai). 54:1244–1256. 2022.PubMed/NCBI

39 

Lewuillon C, Laguillaumie MO, Quesnel B, Idziorek T, Touil Y and Lemonnier L: Put in a ‘Ca2+ll’ to acute myeloid leukemia. Cells. 11:5432022. View Article : Google Scholar : PubMed/NCBI

40 

Apáti A, Jánossy J, Brózik A, Bauer PI and Magócsi M: Calcium induces cell survival and proliferation through the activation of the MAPK pathway in a human hormone-dependent leukemia cell line, TF-1. J Biol Chem. 278:9235–9243. 2003. View Article : Google Scholar : PubMed/NCBI

41 

Lyakh LA, Koski GK, Telford W, Gress RE, Cohen PA and Rice NR: Bacterial lipopolysaccharide, TNF-alpha, and calcium ionophore under serum-free conditions promote rapid dendritic cell-like differentiation in CD14+ monocytes through distinct pathways that activate NK-kappa B. J Immunol. 165:3647–3655. 2000. View Article : Google Scholar : PubMed/NCBI

42 

Kurinna S, Konopleva M, Palla SL, Chen W, Kornblau S, Contractor R, Deng X, May WS, Andreeff M and Ruvolo PP: Bcl2 phosphorylation and active PKC alpha are associated with poor survival in AML. Leukemia. 20:1316–1319. 2006. View Article : Google Scholar : PubMed/NCBI

43 

Cassinelli G, Torri G and Naggi A: Non-anticoagulant heparins as heparanase inhibitors. Adv Exp Med Biol. 1221:493–522. 2020. View Article : Google Scholar : PubMed/NCBI

44 

Wang M, Pan W, Xu Y, Zhang J, Wan J and Jiang H: Microglia-mediated neuroinflammation: A potential target for the treatment of cardiovascular diseases. J Inflamm Res. 15:3083–3094. 2022. View Article : Google Scholar : PubMed/NCBI

45 

Blöchl C, Wang D, Madunić K, Lageveen-Kammeijer GSM, Huber CG, Wuhrer M and Zhang T: Integrated N- and O-glycomics of acute myeloid leukemia (AML) cell lines. Cells. 10:30582021. View Article : Google Scholar : PubMed/NCBI

46 

Corcoran A, Bermudez MA, Seoane S, Perez-Fernandez R, Krupa M, Pietraszek A, Chodyński M, Kutner A, Brown G and Marcinkowska E: Biological evaluation of new vitamin D2 analogues. J Steroid Biochem Mol Biol. 164:66–71. 2016. View Article : Google Scholar : PubMed/NCBI

47 

Liu J, Wei Y, Jia W, Can C, Wang R, Yang X, Gu C, Liu F, Ji C and Ma D: Chenodeoxycholic acid suppresses AML progression through promoting lipid peroxidation via ROS/p38 MAPK/DGAT1 pathway and inhibiting M2 macrophage polarization. Redox Biol. 56:1024522022. View Article : Google Scholar : PubMed/NCBI

48 

Ung J, Tan SF, Fox TE, Shaw JJP, Vass LR, Costa-Pinheiro P, Garrett-Bakelman FE, Keng MK, Sharma A, Claxton DF, et al: Harnessing the power of sphingolipids: Prospects for acute myeloid leukemia. Blood Rev. 55:1009502022. View Article : Google Scholar : PubMed/NCBI

49 

van Galen P, Hovestadt V, Wadsworth Ii MH, Hughes TK, Griffin GK, Battaglia S, Verga JA, Stephansky J, Pastika TJ, Lombardi Story J, et al: Single-Cell RNA-Seq Reveals AML Hierarchies Relevant to Disease Progression and Immunity. Cell. 176:1265–1281.e24. 2019. View Article : Google Scholar : PubMed/NCBI

50 

Kittang AO, Kordasti S, Sand KE, Costantini B, Kramer AM, Perezabellan P, Seidl T, Rye KP, Hagen KM, Kulasekararaj A, et al: Expansion of myeloid derived suppressor cells correlates with number of T regulatory cells and disease progression in myelodysplastic syndrome. Oncoimmunology. 5:e10622082016. View Article : Google Scholar : PubMed/NCBI

51 

Blokland SLM, Kislat A, Homey B, Smithson GM, Kruize AA, Radstake T and van Roon JAG: Decreased circulating CXCR3 + CCR9+T helper cells are associated with elevated levels of their ligands CXCL10 and CCL25 in the salivary gland of patients with Sjögren's syndrome to facilitate their concerted migration. Scand J Immunol. 91:e128522020. View Article : Google Scholar : PubMed/NCBI

52 

Yuan Y, Tan S, Wang H, Zhu J, Li J, Zhang P, Wang M and Zhang F: Mesenchymal stem cell-derived exosomal miRNA-222-3p increases Th1/Th2 ratio and promotes apoptosis of acute myeloid leukemia cells. Anal Cell Pathol (Amst). 2023:40248872023. View Article : Google Scholar : PubMed/NCBI

53 

Zhang F, Zhu K, Liu L, Zhu J, Li J, Zhang P, Hu Z and Yuan Y: IRF2-INPP4B axis inhibits apoptosis of acute myeloid leukaemia cells via regulating T helper 1/2 cell differentiation. Cell Biochem Funct. 38:582–590. 2020. View Article : Google Scholar : PubMed/NCBI

54 

Marsland BJ and Kopf M: T-cell fate and function: PKC-theta and beyond. Trends Immunol. 29:179–185. 2008. View Article : Google Scholar : PubMed/NCBI

55 

Yu D, Peng X and Li P: The correlation between Jun N-terminal kinase pathway-associated phosphatase and Th1 cell or Th17 cell in sepsis and their potential roles in clinical sepsis management. Ir J Med Sci. 190:1173–1181. 2021. View Article : Google Scholar : PubMed/NCBI

56 

Panuciak K, Margas M, Makowska K and Lejman M: Insights into modern therapeutic approaches in pediatric acute leukemias. Cells. 11:1392022. View Article : Google Scholar : PubMed/NCBI

57 

Zhang P, Wang T, Cui G, Ye R, Wan W, Liu T, Zheng Y and Zhong Z: Systemic multifunctional nanovaccines for potent personalized immunotherapy of acute myeloid leukemia. Adv Mater. 36:e24071892024. View Article : Google Scholar : PubMed/NCBI

58 

Gharagozloo M, Mahvelati TM, Imbeault E, Gris P, Zerif E, Bobbala D, Ilangumaran S, Amrani A and Gris D: The nod-like receptor, Nlrp12, plays an anti-inflammatory role in experimental autoimmune encephalomyelitis. J Neuroinflammation. 12:1982015. View Article : Google Scholar : PubMed/NCBI

59 

Puig-Kröger A, López-Rodriguez C, Relloso M, Sánchez-Elsner T, Nueda A, Muñoz E, Bernabéu C and Corbi AL: Polyomavirus enhancer-binding protein 2/core binding factor/acute myeloid leukemia factors contribute to the cell type-specific activity of the CD11a integrin gene promoter. J Biol Chem. 275:28507–28512. 2000. View Article : Google Scholar : PubMed/NCBI

60 

Feng W, Chen J, Huang W, Wang G, Chen X, Duan L, Yin Y, Chen X, Zhang B, Sun M, et al: HMGB1-mediated elevation of KLF7 facilitates hepatocellular carcinoma progression and metastasis through upregulating TLR4 and PTK2. Theranostics. 13:4042–4058. 2023. View Article : Google Scholar : PubMed/NCBI

61 

Pham TND, Perez White BE, Zhao H, Mortazavi F and Tonetti DA: Protein kinase C α enhances migration of breast cancer cells through FOXC2-mediated repression of p120-catenin. BMC Cancer. 17:8322017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yin R, Xu R, Ming H and Song J: Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level. Oncol Lett 30: 489, 2025.
APA
Yin, R., Xu, R., Ming, H., & Song, J. (2025). Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level. Oncology Letters, 30, 489. https://doi.org/10.3892/ol.2025.15235
MLA
Yin, R., Xu, R., Ming, H., Song, J."Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level". Oncology Letters 30.4 (2025): 489.
Chicago
Yin, R., Xu, R., Ming, H., Song, J."Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level". Oncology Letters 30, no. 4 (2025): 489. https://doi.org/10.3892/ol.2025.15235
Copy and paste a formatted citation
x
Spandidos Publications style
Yin R, Xu R, Ming H and Song J: Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level. Oncol Lett 30: 489, 2025.
APA
Yin, R., Xu, R., Ming, H., & Song, J. (2025). Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level. Oncology Letters, 30, 489. https://doi.org/10.3892/ol.2025.15235
MLA
Yin, R., Xu, R., Ming, H., Song, J."Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level". Oncology Letters 30.4 (2025): 489.
Chicago
Yin, R., Xu, R., Ming, H., Song, J."Functional analysis of the effect of isoimperatorin on human acute monocytic leukemia at the transcriptome level". Oncology Letters 30, no. 4 (2025): 489. https://doi.org/10.3892/ol.2025.15235
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team