Differential expression of tetraspanin CD9 in basal cell and squamous cell carcinomas of the skin and actinic keratosis

Ach,Thomas; Ziemer,Mirjana; Dawczynski,Jens; Strobel,Jürgen; Sauer,Georg; Deissler,Helmut

doi:10.3892/ol_00000006

January-February 2010 Volume 1 Issue 1

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January-February 2010 Volume 1 Issue 1

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Article

Differential expression of tetraspanin CD9 in basal cell and squamous cell carcinomas of the skin and actinic keratosis

Authors:
- Thomas Ach
- Mirjana Ziemer
- Jens Dawczynski
- Jürgen Strobel
- Georg Sauer
- Helmut Deissler
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, University Hospital Heidelberg, Heidelberg, Germany
Pages: 37-40
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Published online on: January 1, 2010

https://doi.org/10.3892/ol_00000006
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Abstract

Tetraspanins are potentially useful molecular markers that differentiate between tumour classes and subtypes, since members of this protein family were often found to be altered during malignant conversion and tumour progression. In this study, we analysed expression of the tetraspanin CD9 in the frequent cutaneous neoplasms basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and actinic keratosis (AK), which is considered a precursor lesion (carcinoma in situ) from which an invasive SCC can develop. A moderate to strong CD9-specific staining of the tumour cells' plasma membranes was uniquely observed in all BCCs, SCCs and AKs. All SCCs showed additional intracellular CD9 which was rarely (20%) seen in AKs. Semi-quantitative assessment of CD9 present in the plasma membranes of tumour cells of BCCs (mean staining intensity 1.91) and SCCs (3.64) reflected the different CD9 expression of normal precursor cells from which these tumours most likely originate. Although considered an intermediate stage in the development of SCCs, AKs did not show intense staining of the plasma membranes typical of normal keratinocytes or invasive SCCs (p=0.011) but only moderate intensity (mean 1.63). In BCCs, significantly (p=0.0005, n=56) stronger CD9-specific immunoreactivity was seen in the inner regions of the tumours than at their sites of expansion. In summary, our results point to an important role of CD9 at the front of tumour expansion in BCCs and SCCs, and in the pathogenesis of invasive SCCs.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Differential expression of tetraspanin CD9 in basal cell and squamous cell carcinomas of the skin and actinic keratosis

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