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Article

Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice

  • Authors:
    • Hijiri Takeuchi
    • Kousuke Saoo
    • Keiko Yamakawa
    • Yoko Matsuda
    • Masanao Yokohira
    • Yu Zeng
    • Totshiya Kuno
    • Yukari Totsuka
    • Mami Takahashi
    • Keiji Wakabayashi
    • Katsumi Imaida
  • View Affiliations / Copyright

    Affiliations: Onco-Pathology, Department of Pathology and Host-Defense, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan
  • Pages: 137-142
    |
    Published online on: January 1, 2010
       https://doi.org/10.3892/ol_00000025
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Abstract

It has been reported that 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) induces liver tumors and to a lesser extent lung lesions, lymphomas and leukemias in CDF1 mice. Since a number of case control studies have pointed to a positive association between fat consumption and lung cancer, we examined the lung carcinogenic potential of MeIQx treatment concomitant with a high-fat diet using female A/J mice. Groups 1 and 2 were fed a diet supplemented with MeIQx at a concentration of 600 ppm. Groups 1 and 3 received a diet containing 20% corn oil and group 4 was fed the basal diet alone. After 1 week, 10 mice in each group were sacrificed for measurement of cytochrome P450 (CYP)1A2 mRNA in the liver and lung. The remaining mice were maintained on their respective diets until termination, 32 weeks after the initial MeIQx treatment, when lung proliferative lesions were analyzed. The incidences and multiplicities of hyperplasias and adenomas in MeIQx-treated groups (groups 1 and 2) were significantly higher than in the groups without MeIQx treatment, with a significant increase in the incidences and multiplicities of adenomas + carcinomas, as well as hyperplasia + adenomas + carcinomas (lung proliferative lesions). Lung carcinomas were observed in 1 mouse in each of the MeIQx-treated groups. However, the high-fat diet (groups 1 and 3) did not affect the incidences or multiplicities of lung proliferative lesions. Expression levels of CYP1A2 mRNA after MeIQx treatment significantly increased >3-fold in livers, but no significant change was noted in the lungs, where levels were very low at 1/210 and 1/923 the values for livers. In conclusion, following a 32-week period, we confirmed the lung tumorigenic potential of MeIQx which possibly occurs due to proximate carcinogens activated by CYP1A2 in the liver. However, we failed to detect any influence of a high-fat diet.
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Copy and paste a formatted citation
Spandidos Publications style
Takeuchi H, Saoo K, Yamakawa K, Matsuda Y, Yokohira M, Zeng Y, Kuno T, Totsuka Y, Takahashi M, Wakabayashi K, Wakabayashi K, et al: Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice . Oncol Lett 1: 137-142, 2010.
APA
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y. ... Imaida, K. (2010). Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice . Oncology Letters, 1, 137-142. https://doi.org/10.3892/ol_00000025
MLA
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y., Kuno, T., Totsuka, Y., Takahashi, M., Wakabayashi, K., Imaida, K."Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice ". Oncology Letters 1.1 (2010): 137-142.
Chicago
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y., Kuno, T., Totsuka, Y., Takahashi, M., Wakabayashi, K., Imaida, K."Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice ". Oncology Letters 1, no. 1 (2010): 137-142. https://doi.org/10.3892/ol_00000025
Copy and paste a formatted citation
x
Spandidos Publications style
Takeuchi H, Saoo K, Yamakawa K, Matsuda Y, Yokohira M, Zeng Y, Kuno T, Totsuka Y, Takahashi M, Wakabayashi K, Wakabayashi K, et al: Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice . Oncol Lett 1: 137-142, 2010.
APA
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y. ... Imaida, K. (2010). Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice . Oncology Letters, 1, 137-142. https://doi.org/10.3892/ol_00000025
MLA
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y., Kuno, T., Totsuka, Y., Takahashi, M., Wakabayashi, K., Imaida, K."Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice ". Oncology Letters 1.1 (2010): 137-142.
Chicago
Takeuchi, H., Saoo, K., Yamakawa, K., Matsuda, Y., Yokohira, M., Zeng, Y., Kuno, T., Totsuka, Y., Takahashi, M., Wakabayashi, K., Imaida, K."Tumorigenesis of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), but not enhancing effects of concomitant high-fat diet, on lung carcinogenesis in female A/J mice ". Oncology Letters 1, no. 1 (2010): 137-142. https://doi.org/10.3892/ol_00000025
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