ATP7B expression is associated with in vitro sensitivity to cisplatin in non-small cell lung cancer

Inoue,Yoshimasa; Matsumoto,Hozumi; Yamada,Shunsuke; Kawai,Kenji; Suemizu,Hiroshi; Gika,Masatoshi; Takanami,Iwao; Nakamura,Masato; Iwazaki,Masayuki

doi:10.3892/ol_00000049

ATP7B expression is associated with in vitro sensitivity to cisplatin in non-small cell lung cancer

Authors:
- Yoshimasa Inoue
- Hozumi Matsumoto
- Shunsuke Yamada
- Kenji Kawai
- Hiroshi Suemizu
- Masatoshi Gika
- Iwao Takanami
- Masato Nakamura
- Masayuki Iwazaki
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Affiliations: Department of General Thoracic Surgery, Tokai University Hachioji Hospital, Tokyo 192-0032, Japan
Published online on: March 1, 2010 https://doi.org/10.3892/ol_00000049
Pages: 279-282

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Abstract

Copper-transporting P-type adenosine triphosphatase (ATP7B) is reportedly associated with platinum drug resistance in various solid tumors. However, the impact of ATP7B on platinum drug resistance in non-small cell lung cancer (NSCLC) remains unclear. We investigated in vitro cisplatin (CDDP) sensitivity using the collagen gel-droplet embedded culture drug sensitivity test. The ATP7B mRNA expression level in each specimen was also examined using real-time polymerase chain reaction. The relationship between ATP7B expression and in vitro CDDP sensitivity was then evaluated. The ATP7B mRNA expression levels in CDDP-resistant tumors were significantly higher than those in the CDDP-sensitive group (p=0.015; Mann-Whitney U test). Our results suggested that ATP7B expression is a promising chemoresistance marker for cisplatin.

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