A combination of green tea extract, specific nutrient mixture and quercetin: An effective intervention treatment for the regression of N-methyl-N-nitrosourea (MNU)-induced mammary tumors in Wistar rats

Kale,Anup; Gawande,Sonia; Kotwal,Swati; Netke,Shrirang; Waheed Roomi,M.; Ivanov,Vadim; Niedzwiecki,Aleksandra; Rath,Matthias

doi:10.3892/ol_00000056

March-April 2010 Volume 1 Issue 2

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March-April 2010 Volume 1 Issue 2

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Article

A combination of green tea extract, specific nutrient mixture and quercetin: An effective intervention treatment for the regression of N-methyl-N-nitrosourea (MNU)-induced mammary tumors in Wistar rats

Authors:
- Anup Kale
- Sonia Gawande
- Swati Kotwal
- Shrirang Netke
- M. Waheed Roomi
- Vadim Ivanov
- Aleksandra Niedzwiecki
- Matthias Rath
View Affiliations / Copyright

Affiliations: University Department of Biochemistry, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur 440033, India
Pages: 313-317
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Published online on: March 1, 2010

https://doi.org/10.3892/ol_00000056
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Abstract

Mammary tumors were developed by intraperitoneal injection of N-methyl-N-nitrosourea (MNU) in 21-day-old, sexually immature female Wistar rats. Injection of MNU was repeated 14 weeks after the first one. When palpable tumors were evident in all of the rats, various dietary treatments were initiated for a period of 8 weeks. The treatments were designed to provide 30 mg green tea extract either alone or as a nutrient mixture (E). E was then expanded to include either a nutrient supplement (N), quercetin (Q) or both (N+Q). At the end of the treatment, tumor size/rat measured in the live rats was significantly lower in the groups receiving E, E+Q, E+N and E+N+Q than in the positive control (PC) group which did not receive any dietary treatment. Tumor number/rat, tumor volume/rat and tumor weight/rat were evaluated after sacrificing the rats on the 60th day. The rats receiving E+N+Q showed significantly lower values for the three parameters as compared to the PC group. The PC group showed 24 carcinomas mostly of grade III severity, while the E+N+Q group had only 6 carcinomas, all of which were of grade II severity.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A combination of green tea extract, specific nutrient mixture and quercetin: An effective intervention treatment for the regression of N-methyl-N-nitrosourea (MNU)-induced mammary tumors in Wistar rats

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