Down-regulation of microRNA 10a expression in esophageal squamous cell carcinoma cells

Inoue,Naoki; Isomoto,Hajime; Matsushima,Kayoko; Hayashi,Tomayoshi; Kunizaki,Masaki; Hidaka,Shigekazu; Machida,Haruhisa; Mitsutake,Norisato; Nanashima,Atsushi; Takeshima,Fuminao; Nakayama,Toshiyuki; Ohtsuru,Akira; Nakashima,Masahiro; Nagayasu,Takeshi; Yamashita,Shunichi; Nakao,Kazuhiko; Kohno,Shigeru

doi:10.3892/ol_00000093

May-June 2010 Volume 1 Issue 3

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May-June 2010 Volume 1 Issue 3

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Article

Down-regulation of microRNA 10a expression in esophageal squamous cell carcinoma cells

Authors:
- Naoki Inoue
- Hajime Isomoto
- Kayoko Matsushima
- Tomayoshi Hayashi
- Masaki Kunizaki
- Shigekazu Hidaka
- Haruhisa Machida
- Norisato Mitsutake
- Atsushi Nanashima
- Fuminao Takeshima
- Toshiyuki Nakayama
- Akira Ohtsuru
- Masahiro Nakashima
- Takeshi Nagayasu
- Shunichi Yamashita
- Kazuhiko Nakao
- Shigeru Kohno
View Affiliations / Copyright

Affiliations: Second Department of Internal Medicine, Nagasaki University Hospital, Nagasaki, Japan
Pages: 527-531
|
Published online on: May 1, 2010

https://doi.org/10.3892/ol_00000093
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Abstract

This study identified significantly down-regulated microRNAs (miRs) specific for esophageal squamous cell carcinoma (ESCC) cells. Total RNA was extracted from ESCC cell lines (OE21 and TE10) and a non-malignant human esophageal squamous cell line (Het1A), and subjected to microarray analysis. Expression levels of miRs that showed significant down-regulation in ESCC cells compared to Het1A cells based on the comprehensive analysis were analyzed by quantitative reverse transcription polymerase chain reaction. Among the significantly down-regulated miRs, miR-10a expression levels in the five ESCC cell lines examined were significantly lower than in Het1A and the esophageal adenocarcinoma cells. Since miR-10a is a specific miR in ESCC, its clinical relevance was examined. Using ESCC tumor samples and non-cancerous tissue obtained endoscopically, the involvement of miR-10a in the clinicopathological findings was examined. MiR-10a expression was comparably down-regulated in the tumors of high-grade intraepithelial neoplasm and non-invasive ESCC, while the expression levels were elevated in the invasive ESCC tumors. Treatment with a demethylating agent, 5-aza-2'-deoxycytidine, restored miR-10a expression in OE21 cells. Only a modest additive or synergistic effect was observed in the presence of a histone deacetylase inhibitor, trichostatin A. These results imply that miR-10a may be differentially expressed in ESCC cells and may be involved in ESCC development and progression. The unique epigenetic regulation of miR-10a expression can be mediated via hypermethylation of the CpG islands proximal to its gene locus, at least in certain ESCC cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Down-regulation of microRNA 10a expression in esophageal squamous cell carcinoma cells

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