Pterodon pubescens seed extract induces the cell cycle arrest of leukemic cells by deregulating cyclin D1 and E2 mRNA levels

Pereira,Monica F.; de Almeida Simão,Tatiana; Dalmau,Sérgio R.; Albano,Rodolpho M.; Garcia Pinto Coelho,Marsen; Costa de Carvalho Sabino,Katia

doi:10.3892/ol_00000094

May-June 2010 Volume 1 Issue 3

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-June 2010 Volume 1 Issue 3

Full Size Image

Article

Pterodon pubescens seed extract induces the cell cycle arrest of leukemic cells by deregulating cyclin D1 and E2 mRNA levels

Authors:
- Monica F. Pereira
- Tatiana de Almeida Simão
- Sérgio R. Dalmau
- Rodolpho M. Albano
- Marsen Garcia Pinto Coelho
- Katia Costa de Carvalho Sabino
View Affiliations / Copyright

Affiliations: Departamento de Bioquímica, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, CEP-20550-170 Rio de Janeiro, RJ, Brazil
Pages: 533-536
|
Published online on: May 1, 2010

https://doi.org/10.3892/ol_00000094
Expand metrics +

Abstract

Plant-derived compounds are important sources of effective anti-cancer agents. Pterodon pubescens is a native Brazilian plant popularly known for its anti-inflammatory and anti-arthritic effects. The ethanolic extract of its seeds (EEPp) is a viscous, brown and fragrant oil containing geranylgeraniol, farnesol, naphthalene, dimethyldodecatrienol and vouacapan diterpene derivatives, in addition to other compounds. This study investigated the in vitro anti-leukemic properties of EEPp using the resistant human leukemia cell line K562. The EEPp anti-proliferative effect was demonstrated by the inhibition of DNA synthesis and cell growth, and the induction of cell cycle arrest in the G1 phase. Furthermore, cyclin E2 mRNA levels were down-regulated, while those of cyclin D1 were up-regulated. An EEPp anti-leukemic effect may have also triggered apoptosis, as it increased the number of shrunken cells and phosphatidylserine cell membrane exposure. These observations suggest that EEPp deregulates cyclin D1 and E2 expression, inducing cell cycle arrest and apoptosis of leukemic cells.

View Figures

View References

1	Cragg GM and Newman DJ: Plants as a source of anti-cancer agents. J Ethnopharmacol. 100:72–79. 2005.PubMed/NCBI
2	Sabino KCC, Castro FA, Oliveira JCR, Dalmau SRA and Coelho MGP: Successful therapy of collagen-induced arthritis in mice with a hydroalcoholic extract of seeds of Pterodon pubescens. Phytother Res. 13:613–615. 1999. View Article : Google Scholar : PubMed/NCBI
3	Coelho MGP, Marques PR, Gayer CRM, Vaz LCA, Nogueira Neto JF and Sabino KCC: Subacute toxicity evaluation of a hydroalcoholic extract of Pterodon pubescens seeds in mice with collagen-induced arthritis. J Ethnopharmacol. 77:159–164. 2001. View Article : Google Scholar : PubMed/NCBI
4	Coelho MGP, Sabino KCC and Dalmau SR: Immunomodulatory effects of sucupira (Pterodon pubescens) seed infusion on collagen-induced arthritis. Clin Exp Rheumatol. 22:213–218. 2004.
5	Silva MC, Gayer CR, Lopes CS, et al: Acute and topic anti-edematogenic fractions isolated from the seeds of Pterodon pubescens. J Pharm Pharmacol. 56:135–141. 2004. View Article : Google Scholar : PubMed/NCBI
6	Reddy L, Odhav B and Bhoola KD: Natural products for cancer prevention: a global perspective. Pharmacol Ther. 99:1–13. 2003. View Article : Google Scholar : PubMed/NCBI
7	Vieira CR, Marques MF, Soares PR, et al: Antiproliferative activity of Pterodon pubescens Benth. seed oil and its active principle on human melanoma cells. Phytomedicine. 15:528–532. 2008.
8	Okabe H, Lee SH, Phuchareon J, Albertson DG, McCormick F and Tetsu O: A critical role for FBXW8 and MAPK in cyclin D1 degradation and cancer cell proliferation. PLoS ONE. 1:e1282006. View Article : Google Scholar : PubMed/NCBI
9	Diaz-Carballo D, Malak S, Freistühler M, Elmaagacli A, Bardenheuer W and Reusch HP: Nemorosone blocks proliferation and induces apoptosis in leukemia cells. Int J Clin Pharmacol Ther. 46:428–439. 2008. View Article : Google Scholar : PubMed/NCBI
10	Pytel D, Wysocki T and Majsterek I: Comparative study of DNA damage, cell cycle and apoptosis in human K562 and CCRF-CEM leukemia cells: role of BCR/ABL I therapeutic resistance. Comp Biochem Physiol. 144:85–92. 2006.PubMed/NCBI
11	Miquel K, Pradines A, Tercé F, Selmi S and Favre G: Competitive inhibition of choline phosphotransferase by geranylgeraniol and farnesol inhibits phosphatidylcholine synthesis and induces apoptosis in human lung adenocarcinoma A549 cells. J Biol Chem. 40:26179–26186. 1998. View Article : Google Scholar
12	Masuda Y, Nakaya M, Aiuchi T, Hashimoto S, Nakajo S and Nakaya K: The mechanism of geranylgeraniol-induced apoptosis involves activation, by a caspase-3-like protease, of a c-Jun N-terminal kinase signaling cascade and differs from mechanisms of apoptosis induced by conventional chemotherapeutic drugs. Leuk Res. 24:937–950. 2000. View Article : Google Scholar
13	Rioja A, Pizzey AR, Marson CM and Thomas NS: Preferential induction of apoptosis of leukaemic cells by farnesol. FEBS Lett. 467:291–295. 2000. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Pterodon pubescens seed extract induces the cell cycle arrest of leukemic cells by deregulating cyclin D1 and E2 mRNA levels

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles