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Article

Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells

  • Authors:
    • Hans M. Rodermond
    • Rosemarie ten Cate
    • Jaap Haveman
    • André van Kuilenburg
    • Jan P. Medema
    • Chris van Bree
    • Nicolaas A.P. Franken
  • View Affiliations / Copyright

    Affiliations: Laboratory for Experimental Oncology and Radiobiology (LEXOR), Department of Radiation Oncology, Center for Experimental Molecular Medicine, P.O. Box 22700, 1100 DE Amsterdam, The Netherlands
  • Pages: 537-540
    |
    Published online on: May 1, 2010
       https://doi.org/10.3892/ol_00000095
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Abstract

The search for agents that enhance the effect of ionizing radiation has been an object of study for decades. In this study, the sensitizing properties of cyclopentenylcytosine (CPEC) on radiation and cisplatin-induced radiosensitization in human squamous lung carcinoma cells were investigated. Human lung tumour SW-1573 cells (SWp, parental; SWg, gemcitabine-resistant) were incubated with CPEC and cisplatin and subsequently irradiated with different doses of γ-rays. Clonogenic survival was determined to measure the effectiveness of the treatments. CPEC (1 or 2 µM) treatment for 4 h decreased the plating efficiency to 75 and 50% in SWp and SWg cells, respectively. In the SWg cells, 0.1 and 1 µM CPEC for 4 h enhanced the cell killing effect of cisplatin. However, an increase was not noted in the SWp cells. Due to the moderate toxicity of 1 µM for 4 h, this CPEC dose was used in the radiosensitization experiments. However, CPEC neither radiosensitized the lung tumour cells nor enhanced the radiosensitizing effect of cisplatin. A 2-h incubation with 4 µM cisplatin also decreased the plating efficiency to 75-80% in the two cell lines. Using this cisplatin dose, radiosensitization was obtained in the two cell lines. Although cisplatin treatment clearly radiosensitized the lung tumour cells, CPEC treatment did not. Cisplatin-induced radiosensitization was also not enhanced by CPEC.
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Copy and paste a formatted citation
Spandidos Publications style
Rodermond HM, ten Cate R, Haveman J, van Kuilenburg A, Medema JP, van Bree C and Franken NA: Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells . Oncol Lett 1: 537-540, 2010.
APA
Rodermond, H.M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J.P., van Bree, C., & Franken, N.A. (2010). Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells . Oncology Letters, 1, 537-540. https://doi.org/10.3892/ol_00000095
MLA
Rodermond, H. M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J. P., van Bree, C., Franken, N. A."Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells ". Oncology Letters 1.3 (2010): 537-540.
Chicago
Rodermond, H. M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J. P., van Bree, C., Franken, N. A."Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells ". Oncology Letters 1, no. 3 (2010): 537-540. https://doi.org/10.3892/ol_00000095
Copy and paste a formatted citation
x
Spandidos Publications style
Rodermond HM, ten Cate R, Haveman J, van Kuilenburg A, Medema JP, van Bree C and Franken NA: Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells . Oncol Lett 1: 537-540, 2010.
APA
Rodermond, H.M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J.P., van Bree, C., & Franken, N.A. (2010). Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells . Oncology Letters, 1, 537-540. https://doi.org/10.3892/ol_00000095
MLA
Rodermond, H. M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J. P., van Bree, C., Franken, N. A."Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells ". Oncology Letters 1.3 (2010): 537-540.
Chicago
Rodermond, H. M., ten Cate, R., Haveman, J., van Kuilenburg, A., Medema, J. P., van Bree, C., Franken, N. A."Cyclopentenylcytosine does not enhance cisplatin-induced radiosensitization in human lung tumour cells ". Oncology Letters 1, no. 3 (2010): 537-540. https://doi.org/10.3892/ol_00000095
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