Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis

  • Authors:
    • Hye S. Han
    • Kyung-Yung Lee
    • So D. Lim
    • Wan S. Kim
    • Tae S. Hwang
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  • Published online on: May 1, 2010     https://doi.org/10.3892/ol_00000098
  • Pages: 555-558
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Abstract

Helicobacter pylori (H. pylori) is a microaerophilic gram-negative bacterium known to be associated with chronic gastritis, peptic ulcer and gastric adenocarcinoma. In the present study, the presence of Helicobacter DNA was investigated using a Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis in 51 resected gastric adenocarcinomas. DNA was extracted from paraffin-embedded tissues of resected gastric adenocarcinomas. PCR primers were designed to amplify the 133-bp PCR fragment in highly conserved regions of the 16S rRNA gene. The sequence of the PCR products was analyzed using a PSQ 96 system with SQA software. The pyrosequencing analysis of 16S rRNA showed that H. pylori was present in 47 (92.2%) of the 51 gastric adenocarcinomas. In the 4 H. pylori-negative cases, Helicobacter cinaedi (2 cases), Helicobacter mustelae (1 case) and Campylobacter hyointestinalis (1 case) were detected. Pyrosequencing technology was useful in the identification and differentiation of H. pylori from other species by analyzing the gene encoding 16S rRNA. Gastric adenocarcinoma tissues contain bacteria, and the majority are H. pylori. Helicobacter cinaedi, Helicobacter mustelae and Campylobacter hyointestinalis rarely occur. The roles of these organisms in the pathogenesis of gastric adenocarcinoma remain unclear.
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May-June 2010
Volume 1 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Han HS, Lee K, Lim SD, Kim WS and Hwang TS: Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis . Oncol Lett 1: 555-558, 2010.
APA
Han, H.S., Lee, K., Lim, S.D., Kim, W.S., & Hwang, T.S. (2010). Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis . Oncology Letters, 1, 555-558. https://doi.org/10.3892/ol_00000098
MLA
Han, H. S., Lee, K., Lim, S. D., Kim, W. S., Hwang, T. S."Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis ". Oncology Letters 1.3 (2010): 555-558.
Chicago
Han, H. S., Lee, K., Lim, S. D., Kim, W. S., Hwang, T. S."Molecular identification of Helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis ". Oncology Letters 1, no. 3 (2010): 555-558. https://doi.org/10.3892/ol_00000098